Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells

Autores
Paula, Flavia M.M.; Barbosa, Helena C.L.; Carneiro, Everardo M.; Persaud, Shanta J.; Gagliardino, Juan Jose; Boschero, Antonio C.; Souza, Kleber L.A.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pentadecapeptide comprising the 104–118 amino acid sequence of the ilotropin-derived Reg3-related islet neogenesis-associated protein (INGAP-PP) has been implicated in beta cell neogenesis and enhancement of insulin secretion in pancreatic islets. The aim of this study was to investigate intracellular pathways by which INGAP-PP signals in insulin-producing cells. Treatment with INGAP-PP increased insulin secretion and intracellular calcium levels in MIN6 cells. INGAP-PP exposure activated c-Myc, serum and particularly nuclear factor-kappaB (NF-κB) response elements in insulin-producing cells (1.7 ± 0.1, 1.8 ± 0.1, 2.4 ± 0.3 for RINm5F, and 1.3 ± 0.1, 1.3 ± 0.1 and 1.6 ± 0.1 fold for MIN6 cells compared to controls, respectively). There was an increase in the proliferation rate of viable cells (162 ± 17% for RINm5F and 155 ± 13% for MIN6) that was accompanied by an increase in proliferating cell nuclear antigen (PCNA) protein expression (187 ± 19% and 170 ± 8% for RINm5F and MIN6 cells respectively) following INGAP-PP treatment. INGAP-PP increased the expression of the muscarinic M3 receptor subtype (169 ± 4% for RINm5F and 222 ± 20% for MIN6 cells). Activation of multiple serum response elements by foetal calf serum also increased muscarinic M3 receptor expression (173 ± 9% for RINm5F and 140 ± 7% for MIN6 cells). The blockade of NF-κB signalling pathway strongly decreased muscarinic M3 receptor expression in response to both stimuli. In summary, a network of intracellular signals that includes activation of c-Myc signalling pathway and increased PCNA expression might be related to the increased proliferation rate of insulin-producing cells following incubation with INGAP-PP. NF-κB signalling plays an essential role in controlling the expression of the muscarinic M3 receptor.
Fil: Paula, Flavia M.M.. Universidade Estadual de Campinas; Brasil
Fil: Barbosa, Helena C.L.. Universidade Estadual de Campinas; Brasil
Fil: Carneiro, Everardo M.. Universidade Estadual de Campinas; Brasil
Fil: Persaud, Shanta J.. King's College London; Reino Unido
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina
Fil: Boschero, Antonio C.. Universidade Estadual de Campinas; Brasil
Fil: Souza, Kleber L.A.. Universidade Estadual de Campinas; Brasil
Materia
CHOLINERGIC RECEPTOR
DIABETES
GENE EXPRESSION REGULATION
MACHR
PSEUDOISLET
TRANSCRIPTION FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95543
Acceder
id CONICETDig_0464d9367552cbad050c9de0238a9a3f
oai_identifier_str oai:ri.conicet.gov.ar:11336/95543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cellsPaula, Flavia M.M.Barbosa, Helena C.L.Carneiro, Everardo M.Persaud, Shanta J.Gagliardino, Juan JoseBoschero, Antonio C.Souza, Kleber L.A.CHOLINERGIC RECEPTORDIABETESGENE EXPRESSION REGULATIONMACHRPSEUDOISLETTRANSCRIPTION FACTORhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The pentadecapeptide comprising the 104–118 amino acid sequence of the ilotropin-derived Reg3-related islet neogenesis-associated protein (INGAP-PP) has been implicated in beta cell neogenesis and enhancement of insulin secretion in pancreatic islets. The aim of this study was to investigate intracellular pathways by which INGAP-PP signals in insulin-producing cells. Treatment with INGAP-PP increased insulin secretion and intracellular calcium levels in MIN6 cells. INGAP-PP exposure activated c-Myc, serum and particularly nuclear factor-kappaB (NF-κB) response elements in insulin-producing cells (1.7 ± 0.1, 1.8 ± 0.1, 2.4 ± 0.3 for RINm5F, and 1.3 ± 0.1, 1.3 ± 0.1 and 1.6 ± 0.1 fold for MIN6 cells compared to controls, respectively). There was an increase in the proliferation rate of viable cells (162 ± 17% for RINm5F and 155 ± 13% for MIN6) that was accompanied by an increase in proliferating cell nuclear antigen (PCNA) protein expression (187 ± 19% and 170 ± 8% for RINm5F and MIN6 cells respectively) following INGAP-PP treatment. INGAP-PP increased the expression of the muscarinic M3 receptor subtype (169 ± 4% for RINm5F and 222 ± 20% for MIN6 cells). Activation of multiple serum response elements by foetal calf serum also increased muscarinic M3 receptor expression (173 ± 9% for RINm5F and 140 ± 7% for MIN6 cells). The blockade of NF-κB signalling pathway strongly decreased muscarinic M3 receptor expression in response to both stimuli. In summary, a network of intracellular signals that includes activation of c-Myc signalling pathway and increased PCNA expression might be related to the increased proliferation rate of insulin-producing cells following incubation with INGAP-PP. NF-κB signalling plays an essential role in controlling the expression of the muscarinic M3 receptor.Fil: Paula, Flavia M.M.. Universidade Estadual de Campinas; BrasilFil: Barbosa, Helena C.L.. Universidade Estadual de Campinas; BrasilFil: Carneiro, Everardo M.. Universidade Estadual de Campinas; BrasilFil: Persaud, Shanta J.. King's College London; Reino UnidoFil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); ArgentinaFil: Boschero, Antonio C.. Universidade Estadual de Campinas; BrasilFil: Souza, Kleber L.A.. Universidade Estadual de Campinas; BrasilElsevier Science2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95543Paula, Flavia M.M.; Barbosa, Helena C.L.; Carneiro, Everardo M.; Persaud, Shanta J.; Gagliardino, Juan Jose; et al.; Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells; Elsevier Science; European Journal of Pharmacology; 642; 1-3; 9-2010; 37-460014-2999CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014299910005078info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2010.05.056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:43Zoai:ri.conicet.gov.ar:11336/95543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:43.989CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
title Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
spellingShingle Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
Paula, Flavia M.M.
CHOLINERGIC RECEPTOR
DIABETES
GENE EXPRESSION REGULATION
MACHR
PSEUDOISLET
TRANSCRIPTION FACTOR
title_short Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
title_full Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
title_fullStr Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
title_full_unstemmed Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
title_sort Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells
dc.creator.none.fl_str_mv Paula, Flavia M.M.
Barbosa, Helena C.L.
Carneiro, Everardo M.
Persaud, Shanta J.
Gagliardino, Juan Jose
Boschero, Antonio C.
Souza, Kleber L.A.
author Paula, Flavia M.M.
author_facet Paula, Flavia M.M.
Barbosa, Helena C.L.
Carneiro, Everardo M.
Persaud, Shanta J.
Gagliardino, Juan Jose
Boschero, Antonio C.
Souza, Kleber L.A.
author_role author
author2 Barbosa, Helena C.L.
Carneiro, Everardo M.
Persaud, Shanta J.
Gagliardino, Juan Jose
Boschero, Antonio C.
Souza, Kleber L.A.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHOLINERGIC RECEPTOR
DIABETES
GENE EXPRESSION REGULATION
MACHR
PSEUDOISLET
TRANSCRIPTION FACTOR
topic CHOLINERGIC RECEPTOR
DIABETES
GENE EXPRESSION REGULATION
MACHR
PSEUDOISLET
TRANSCRIPTION FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The pentadecapeptide comprising the 104–118 amino acid sequence of the ilotropin-derived Reg3-related islet neogenesis-associated protein (INGAP-PP) has been implicated in beta cell neogenesis and enhancement of insulin secretion in pancreatic islets. The aim of this study was to investigate intracellular pathways by which INGAP-PP signals in insulin-producing cells. Treatment with INGAP-PP increased insulin secretion and intracellular calcium levels in MIN6 cells. INGAP-PP exposure activated c-Myc, serum and particularly nuclear factor-kappaB (NF-κB) response elements in insulin-producing cells (1.7 ± 0.1, 1.8 ± 0.1, 2.4 ± 0.3 for RINm5F, and 1.3 ± 0.1, 1.3 ± 0.1 and 1.6 ± 0.1 fold for MIN6 cells compared to controls, respectively). There was an increase in the proliferation rate of viable cells (162 ± 17% for RINm5F and 155 ± 13% for MIN6) that was accompanied by an increase in proliferating cell nuclear antigen (PCNA) protein expression (187 ± 19% and 170 ± 8% for RINm5F and MIN6 cells respectively) following INGAP-PP treatment. INGAP-PP increased the expression of the muscarinic M3 receptor subtype (169 ± 4% for RINm5F and 222 ± 20% for MIN6 cells). Activation of multiple serum response elements by foetal calf serum also increased muscarinic M3 receptor expression (173 ± 9% for RINm5F and 140 ± 7% for MIN6 cells). The blockade of NF-κB signalling pathway strongly decreased muscarinic M3 receptor expression in response to both stimuli. In summary, a network of intracellular signals that includes activation of c-Myc signalling pathway and increased PCNA expression might be related to the increased proliferation rate of insulin-producing cells following incubation with INGAP-PP. NF-κB signalling plays an essential role in controlling the expression of the muscarinic M3 receptor.
Fil: Paula, Flavia M.M.. Universidade Estadual de Campinas; Brasil
Fil: Barbosa, Helena C.L.. Universidade Estadual de Campinas; Brasil
Fil: Carneiro, Everardo M.. Universidade Estadual de Campinas; Brasil
Fil: Persaud, Shanta J.. King's College London; Reino Unido
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina
Fil: Boschero, Antonio C.. Universidade Estadual de Campinas; Brasil
Fil: Souza, Kleber L.A.. Universidade Estadual de Campinas; Brasil
description The pentadecapeptide comprising the 104–118 amino acid sequence of the ilotropin-derived Reg3-related islet neogenesis-associated protein (INGAP-PP) has been implicated in beta cell neogenesis and enhancement of insulin secretion in pancreatic islets. The aim of this study was to investigate intracellular pathways by which INGAP-PP signals in insulin-producing cells. Treatment with INGAP-PP increased insulin secretion and intracellular calcium levels in MIN6 cells. INGAP-PP exposure activated c-Myc, serum and particularly nuclear factor-kappaB (NF-κB) response elements in insulin-producing cells (1.7 ± 0.1, 1.8 ± 0.1, 2.4 ± 0.3 for RINm5F, and 1.3 ± 0.1, 1.3 ± 0.1 and 1.6 ± 0.1 fold for MIN6 cells compared to controls, respectively). There was an increase in the proliferation rate of viable cells (162 ± 17% for RINm5F and 155 ± 13% for MIN6) that was accompanied by an increase in proliferating cell nuclear antigen (PCNA) protein expression (187 ± 19% and 170 ± 8% for RINm5F and MIN6 cells respectively) following INGAP-PP treatment. INGAP-PP increased the expression of the muscarinic M3 receptor subtype (169 ± 4% for RINm5F and 222 ± 20% for MIN6 cells). Activation of multiple serum response elements by foetal calf serum also increased muscarinic M3 receptor expression (173 ± 9% for RINm5F and 140 ± 7% for MIN6 cells). The blockade of NF-κB signalling pathway strongly decreased muscarinic M3 receptor expression in response to both stimuli. In summary, a network of intracellular signals that includes activation of c-Myc signalling pathway and increased PCNA expression might be related to the increased proliferation rate of insulin-producing cells following incubation with INGAP-PP. NF-κB signalling plays an essential role in controlling the expression of the muscarinic M3 receptor.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95543
Paula, Flavia M.M.; Barbosa, Helena C.L.; Carneiro, Everardo M.; Persaud, Shanta J.; Gagliardino, Juan Jose; et al.; Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells; Elsevier Science; European Journal of Pharmacology; 642; 1-3; 9-2010; 37-46
0014-2999
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95543
identifier_str_mv Paula, Flavia M.M.; Barbosa, Helena C.L.; Carneiro, Everardo M.; Persaud, Shanta J.; Gagliardino, Juan Jose; et al.; Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M3 receptor expression by INGAP-PP in insulin-producing cells; Elsevier Science; European Journal of Pharmacology; 642; 1-3; 9-2010; 37-46
0014-2999
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014299910005078
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2010.05.056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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