Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications

Autores
Reina, Silvia Lorena; Sterin Borda, Leonor; Orman, Betina; Borda, Enri Santiago
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies have demonstrated that antibodies against muscarinic acetylcholine receptors (mAChRs) from exocrine glands, correlates with Sjögren syndrome (SS) in the majority of patients. The aim of the present investigation was to establish if serum IgG antibodies present in SS interacts with cerebral mAChRs. Results show that anti-cerebral IgG are present in the sera of 40% SS patients studied. Autoantibodies were able to interact with mAChRs of cerebral frontal cortex membranes inhibiting the [(3)H]QNB binding to its specific receptor. Moreover, tested by ELISA and dot blot they recognized the synthetic peptides corresponding to the second extracellular loop of human M(1) and M(3) mAChR. In addition, the corresponding affinity-purified anti-M(1) and anti-M(3) peptide IgGs displayed an agonistic activity, stimulating phosphoinositide hydrolysis. The results support the notion that serum IgG autoantibodies in SS patients target cerebral mAChRs may have some role in the pathogenesis of higher cognitive dysfunction present in SS patients.
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Las Misiones; Argentina
Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
CEREBRAL ANTIBODIES
CEREBRAL NOS
CEREBRAL PIS
ELISA
M1 MACHR IGG
M3 MACHR IGG
MACHRS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163922
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implicationsReina, Silvia LorenaSterin Borda, LeonorOrman, BetinaBorda, Enri SantiagoCEREBRAL ANTIBODIESCEREBRAL NOSCEREBRAL PISELISAM1 MACHR IGGM3 MACHR IGGMACHRShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous studies have demonstrated that antibodies against muscarinic acetylcholine receptors (mAChRs) from exocrine glands, correlates with Sjögren syndrome (SS) in the majority of patients. The aim of the present investigation was to establish if serum IgG antibodies present in SS interacts with cerebral mAChRs. Results show that anti-cerebral IgG are present in the sera of 40% SS patients studied. Autoantibodies were able to interact with mAChRs of cerebral frontal cortex membranes inhibiting the [(3)H]QNB binding to its specific receptor. Moreover, tested by ELISA and dot blot they recognized the synthetic peptides corresponding to the second extracellular loop of human M(1) and M(3) mAChR. In addition, the corresponding affinity-purified anti-M(1) and anti-M(3) peptide IgGs displayed an agonistic activity, stimulating phosphoinositide hydrolysis. The results support the notion that serum IgG autoantibodies in SS patients target cerebral mAChRs may have some role in the pathogenesis of higher cognitive dysfunction present in SS patients.Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Las Misiones; ArgentinaFil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Orman, Betina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Science2004-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163922Reina, Silvia Lorena; Sterin Borda, Leonor; Orman, Betina; Borda, Enri Santiago; Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications; Elsevier Science; Journal of Neuroimmunology; 150; 1-2; 5-2004; 107-1150165-5728CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165572804000086info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneuroim.2004.01.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:56Zoai:ri.conicet.gov.ar:11336/163922instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:56.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
title Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
spellingShingle Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
Reina, Silvia Lorena
CEREBRAL ANTIBODIES
CEREBRAL NOS
CEREBRAL PIS
ELISA
M1 MACHR IGG
M3 MACHR IGG
MACHRS
title_short Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
title_full Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
title_fullStr Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
title_full_unstemmed Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
title_sort Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications
dc.creator.none.fl_str_mv Reina, Silvia Lorena
Sterin Borda, Leonor
Orman, Betina
Borda, Enri Santiago
author Reina, Silvia Lorena
author_facet Reina, Silvia Lorena
Sterin Borda, Leonor
Orman, Betina
Borda, Enri Santiago
author_role author
author2 Sterin Borda, Leonor
Orman, Betina
Borda, Enri Santiago
author2_role author
author
author
dc.subject.none.fl_str_mv CEREBRAL ANTIBODIES
CEREBRAL NOS
CEREBRAL PIS
ELISA
M1 MACHR IGG
M3 MACHR IGG
MACHRS
topic CEREBRAL ANTIBODIES
CEREBRAL NOS
CEREBRAL PIS
ELISA
M1 MACHR IGG
M3 MACHR IGG
MACHRS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous studies have demonstrated that antibodies against muscarinic acetylcholine receptors (mAChRs) from exocrine glands, correlates with Sjögren syndrome (SS) in the majority of patients. The aim of the present investigation was to establish if serum IgG antibodies present in SS interacts with cerebral mAChRs. Results show that anti-cerebral IgG are present in the sera of 40% SS patients studied. Autoantibodies were able to interact with mAChRs of cerebral frontal cortex membranes inhibiting the [(3)H]QNB binding to its specific receptor. Moreover, tested by ELISA and dot blot they recognized the synthetic peptides corresponding to the second extracellular loop of human M(1) and M(3) mAChR. In addition, the corresponding affinity-purified anti-M(1) and anti-M(3) peptide IgGs displayed an agonistic activity, stimulating phosphoinositide hydrolysis. The results support the notion that serum IgG autoantibodies in SS patients target cerebral mAChRs may have some role in the pathogenesis of higher cognitive dysfunction present in SS patients.
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Las Misiones; Argentina
Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Previous studies have demonstrated that antibodies against muscarinic acetylcholine receptors (mAChRs) from exocrine glands, correlates with Sjögren syndrome (SS) in the majority of patients. The aim of the present investigation was to establish if serum IgG antibodies present in SS interacts with cerebral mAChRs. Results show that anti-cerebral IgG are present in the sera of 40% SS patients studied. Autoantibodies were able to interact with mAChRs of cerebral frontal cortex membranes inhibiting the [(3)H]QNB binding to its specific receptor. Moreover, tested by ELISA and dot blot they recognized the synthetic peptides corresponding to the second extracellular loop of human M(1) and M(3) mAChR. In addition, the corresponding affinity-purified anti-M(1) and anti-M(3) peptide IgGs displayed an agonistic activity, stimulating phosphoinositide hydrolysis. The results support the notion that serum IgG autoantibodies in SS patients target cerebral mAChRs may have some role in the pathogenesis of higher cognitive dysfunction present in SS patients.
publishDate 2004
dc.date.none.fl_str_mv 2004-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163922
Reina, Silvia Lorena; Sterin Borda, Leonor; Orman, Betina; Borda, Enri Santiago; Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications; Elsevier Science; Journal of Neuroimmunology; 150; 1-2; 5-2004; 107-115
0165-5728
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163922
identifier_str_mv Reina, Silvia Lorena; Sterin Borda, Leonor; Orman, Betina; Borda, Enri Santiago; Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: Functional and pathological implications; Elsevier Science; Journal of Neuroimmunology; 150; 1-2; 5-2004; 107-115
0165-5728
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165572804000086
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneuroim.2004.01.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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