Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae

Autores
Dinolfo, María Inés; Barros, Germán Gustavo; Stenglein, Sebastian Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the tri7 gene to detect potential nivalenol-producing F. poae isolates. A total of 125 F. poae, four F. cerealis, two F. culmorum, one F. langsethiae, one F. sporotrichioides and seven F. graminearum, plus F. austroamericanum, F. meridionale, F. graminearum sensu stricto and F. cortaderiae from the NRRL collection were analysed, and only F. poae isolates gave a positive result for the presence of a 296-bp partial tri7 DNA fragment. Moreover, the primer set was tested from cereal seed samples where F. poae and other Fusarium species with a negative result for the specific reaction (F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti and F. acuminatum) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing F. poae isolates.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Barros, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Stenglein, Sebastian Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Microbiología Clínica y Experimental; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; Argentina
Materia
CEREAL GRAINS
FUSARIUM HEAD BLIGHT
MYCOTOXINS
RAPID DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148907
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/148907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a PCR assay to detect the potential production of nivalenol in Fusarium poaeDinolfo, María InésBarros, Germán GustavoStenglein, Sebastian AlbertoCEREAL GRAINSFUSARIUM HEAD BLIGHTMYCOTOXINSRAPID DETECTIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the tri7 gene to detect potential nivalenol-producing F. poae isolates. A total of 125 F. poae, four F. cerealis, two F. culmorum, one F. langsethiae, one F. sporotrichioides and seven F. graminearum, plus F. austroamericanum, F. meridionale, F. graminearum sensu stricto and F. cortaderiae from the NRRL collection were analysed, and only F. poae isolates gave a positive result for the presence of a 296-bp partial tri7 DNA fragment. Moreover, the primer set was tested from cereal seed samples where F. poae and other Fusarium species with a negative result for the specific reaction (F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti and F. acuminatum) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing F. poae isolates.Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Barros, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Stenglein, Sebastian Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Microbiología Clínica y Experimental; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; ArgentinaWiley Blackwell Publishing, Inc2012-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148907Dinolfo, María Inés; Barros, Germán Gustavo; Stenglein, Sebastian Alberto; Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 332; 2; 15-7-2012; 99-1040378-10971574-6968CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/332/2/99/502573info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02581.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:01Zoai:ri.conicet.gov.ar:11336/148907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:01.704CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
spellingShingle Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
Dinolfo, María Inés
CEREAL GRAINS
FUSARIUM HEAD BLIGHT
MYCOTOXINS
RAPID DETECTION
title_short Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_full Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_fullStr Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_full_unstemmed Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_sort Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
dc.creator.none.fl_str_mv Dinolfo, María Inés
Barros, Germán Gustavo
Stenglein, Sebastian Alberto
author Dinolfo, María Inés
author_facet Dinolfo, María Inés
Barros, Germán Gustavo
Stenglein, Sebastian Alberto
author_role author
author2 Barros, Germán Gustavo
Stenglein, Sebastian Alberto
author2_role author
author
dc.subject.none.fl_str_mv CEREAL GRAINS
FUSARIUM HEAD BLIGHT
MYCOTOXINS
RAPID DETECTION
topic CEREAL GRAINS
FUSARIUM HEAD BLIGHT
MYCOTOXINS
RAPID DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the tri7 gene to detect potential nivalenol-producing F. poae isolates. A total of 125 F. poae, four F. cerealis, two F. culmorum, one F. langsethiae, one F. sporotrichioides and seven F. graminearum, plus F. austroamericanum, F. meridionale, F. graminearum sensu stricto and F. cortaderiae from the NRRL collection were analysed, and only F. poae isolates gave a positive result for the presence of a 296-bp partial tri7 DNA fragment. Moreover, the primer set was tested from cereal seed samples where F. poae and other Fusarium species with a negative result for the specific reaction (F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti and F. acuminatum) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing F. poae isolates.
Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Barros, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Stenglein, Sebastian Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Microbiología Clínica y Experimental; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología; Argentina
description Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the tri7 gene to detect potential nivalenol-producing F. poae isolates. A total of 125 F. poae, four F. cerealis, two F. culmorum, one F. langsethiae, one F. sporotrichioides and seven F. graminearum, plus F. austroamericanum, F. meridionale, F. graminearum sensu stricto and F. cortaderiae from the NRRL collection were analysed, and only F. poae isolates gave a positive result for the presence of a 296-bp partial tri7 DNA fragment. Moreover, the primer set was tested from cereal seed samples where F. poae and other Fusarium species with a negative result for the specific reaction (F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti and F. acuminatum) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing F. poae isolates.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148907
Dinolfo, María Inés; Barros, Germán Gustavo; Stenglein, Sebastian Alberto; Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 332; 2; 15-7-2012; 99-104
0378-1097
1574-6968
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148907
identifier_str_mv Dinolfo, María Inés; Barros, Germán Gustavo; Stenglein, Sebastian Alberto; Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 332; 2; 15-7-2012; 99-104
0378-1097
1574-6968
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/332/2/99/502573
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02581.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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