Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae

Autores
Dinolfo, María Inés; Barros, Germán G.; Stenglein, Sebastián
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Fusariumspecies can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance ofFusarium poaehas increased within theFusariumhead blight complex.Fusarium poaeis known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on thetri7gene to detect potential nivalenol-producingF. poaeisolates. A total of 125F. poae, fourF. cerealis, twoF. culmorum, oneF. langsethiae, oneF. sporotrichioidesand sevenF. graminearum, plusF. austroamericanum, F. meridionale, F. graminearum sensu strictoandF. cortaderiaefrom the NRRL collection were analysed, and onlyF. poaeisolates gave a positive result for the presence of a 296-bp partialtri7DNA fragment. Moreover, the primer set was tested from cereal seed samples whereF. poaeand otherFusariumspecies with a negative result for the specific reaction (F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equisetiandF. acuminatum) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producingF. poaeisolates.
Materia
Biología Celular, Microbiología
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/6437
Acceder
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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/6437
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Development of a PCR assay to detect the potential production of nivalenol in Fusarium poaeDinolfo, María InésBarros, Germán G.Stenglein, SebastiánBiología Celular, Microbiología<em>Fusarium</em>species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of<em>Fusarium poae</em>has increased within the<em>Fusarium</em>head blight complex.<em>Fusarium poae</em>is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the<em>tri7</em>gene to detect potential nivalenol-producing<em>F. poae</em>isolates. A total of 125<em>F. poae</em>, four<em>F. cerealis</em>, two<em>F. culmorum</em>, one<em>F. langsethiae</em>, one<em>F. sporotrichioides</em>and seven<em>F. graminearum</em>, plus<em>F. austroamericanum, F. meridionale, F. graminearum sensu stricto</em>and<em>F. cortaderiae</em>from the NRRL collection were analysed, and only<em>F. poae</em>isolates gave a positive result for the presence of a 296-bp partial<em>tri7</em>DNA fragment. Moreover, the primer set was tested from cereal seed samples where<em>F. poae</em>and other<em>Fusarium</em>species with a negative result for the specific reaction (<em>F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti</em>and<em>F. acuminatum</em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing<em>F. poae</em>isolates.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/6437enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02581.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:43:50Zoai:digital.cic.gba.gob.ar:11746/6437Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:43:51.201CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
spellingShingle Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
Dinolfo, María Inés
Biología Celular, Microbiología
title_short Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_full Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_fullStr Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_full_unstemmed Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
title_sort Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
dc.creator.none.fl_str_mv Dinolfo, María Inés
Barros, Germán G.
Stenglein, Sebastián
author Dinolfo, María Inés
author_facet Dinolfo, María Inés
Barros, Germán G.
Stenglein, Sebastián
author_role author
author2 Barros, Germán G.
Stenglein, Sebastián
author2_role author
author
dc.subject.none.fl_str_mv Biología Celular, Microbiología
topic Biología Celular, Microbiología
dc.description.none.fl_txt_mv <em>Fusarium</em>species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of<em>Fusarium poae</em>has increased within the<em>Fusarium</em>head blight complex.<em>Fusarium poae</em>is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the<em>tri7</em>gene to detect potential nivalenol-producing<em>F. poae</em>isolates. A total of 125<em>F. poae</em>, four<em>F. cerealis</em>, two<em>F. culmorum</em>, one<em>F. langsethiae</em>, one<em>F. sporotrichioides</em>and seven<em>F. graminearum</em>, plus<em>F. austroamericanum, F. meridionale, F. graminearum sensu stricto</em>and<em>F. cortaderiae</em>from the NRRL collection were analysed, and only<em>F. poae</em>isolates gave a positive result for the presence of a 296-bp partial<em>tri7</em>DNA fragment. Moreover, the primer set was tested from cereal seed samples where<em>F. poae</em>and other<em>Fusarium</em>species with a negative result for the specific reaction (<em>F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti</em>and<em>F. acuminatum</em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing<em>F. poae</em>isolates.
description <em>Fusarium</em>species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of<em>Fusarium poae</em>has increased within the<em>Fusarium</em>head blight complex.<em>Fusarium poae</em>is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the<em>tri7</em>gene to detect potential nivalenol-producing<em>F. poae</em>isolates. A total of 125<em>F. poae</em>, four<em>F. cerealis</em>, two<em>F. culmorum</em>, one<em>F. langsethiae</em>, one<em>F. sporotrichioides</em>and seven<em>F. graminearum</em>, plus<em>F. austroamericanum, F. meridionale, F. graminearum sensu stricto</em>and<em>F. cortaderiae</em>from the NRRL collection were analysed, and only<em>F. poae</em>isolates gave a positive result for the presence of a 296-bp partial<em>tri7</em>DNA fragment. Moreover, the primer set was tested from cereal seed samples where<em>F. poae</em>and other<em>Fusarium</em>species with a negative result for the specific reaction (<em>F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti</em>and<em>F. acuminatum</em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing<em>F. poae</em>isolates.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/6437
url https://digital.cic.gba.gob.ar/handle/11746/6437
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02581.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
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collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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