Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes

Autores
Byun, Jung S.; Wong, Madeline M.; Cui, Wenwu; Idelman, Gila; Li, Quentin; de Siervi, Adriana; Bilke, Sven; Haggerty, Cynthia M.; Player, Audrey; Wang, Yong Hong; Thirman, Michael J.; Kaberlein, Joseph J.; Petrovas, Constantinos; Koup, Richard A.; Longo, Dan L.; Ozato, Keiko; Gardner, Kevin
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the control of primary genetic responses. The recruitment of p300 and pol II rapidly transitions to the assembly of several elongation factors, including the positive transcriptional elongation factor (P-TEFb), the bromodomain-containing protein (BRD4), and the elongin-like eleven nineteen lysine-rich leukemia protein (ELL). However, transcription at many of these rapidly induced genes is transient, wherein swift departure of P-TEFb, BRD4, and ELL coincides with termination of transcriptional elongation. Unexpectedly, both p300 and pol II remain accumulated or "bookmarked" at the proximal promoter long after transcription has terminated, demarking a clear mechanistic separation between the recruitment and elongation phases of transcription in vivo. The bookmarked pol II is depleted of both serine-2 and serine-5 phosphorylation of its C-terminal domain and remains proximally positioned at the promoter for hours. Surprisingly, these p300/pol II bookmarked genes can be readily reactivated, and elongation factors can be reassembled by subsequent addition of nonmitogenic agents that, alone, have minimal effects on transcription in the absence of prior preconditioning by mitogen stimulation. These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development through the dynamic priming of genes for response to rechallenge by secondary stimuli.
Fil: Byun, Jung S.. National Cancer Institute; Estados Unidos
Fil: Wong, Madeline M.. National Cancer Institute; Estados Unidos
Fil: Cui, Wenwu. National Cancer Institute; Estados Unidos
Fil: Idelman, Gila. National Cancer Institute; Estados Unidos
Fil: Li, Quentin. National Cancer Institute; Estados Unidos
Fil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bilke, Sven. National Cancer Institute; Estados Unidos
Fil: Haggerty, Cynthia M.. National Cancer Institute; Estados Unidos
Fil: Player, Audrey. National Cancer Institute; Estados Unidos
Fil: Wang, Yong Hong. National Cancer Institute; Estados Unidos
Fil: Thirman, Michael J.. The University Of Chicago Medicine; Estados Unidos
Fil: Kaberlein, Joseph J.. The University Of Chicago Medicine; Estados Unidos
Fil: Petrovas, Constantinos. National Institutes of Health; Estados Unidos
Fil: Koup, Richard A.. National Institutes of Health; Estados Unidos
Fil: Longo, Dan L.. National Institute On Aging; Estados Unidos
Fil: Ozato, Keiko. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Gardner, Kevin. National Cancer Institute; Estados Unidos
Materia
Ell
Epigenetics
Gene Regulation
Histone Acetylation
Transcription
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/83950
Acceder
id CONICETDig_031e84438db9eb5b0f423b7c98395b19
oai_identifier_str oai:ri.conicet.gov.ar:11336/83950
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexesByun, Jung S.Wong, Madeline M.Cui, WenwuIdelman, GilaLi, Quentinde Siervi, AdrianaBilke, SvenHaggerty, Cynthia M.Player, AudreyWang, Yong HongThirman, Michael J.Kaberlein, Joseph J.Petrovas, ConstantinosKoup, Richard A.Longo, Dan L.Ozato, KeikoGardner, KevinEllEpigeneticsGene RegulationHistone AcetylationTranscriptionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the control of primary genetic responses. The recruitment of p300 and pol II rapidly transitions to the assembly of several elongation factors, including the positive transcriptional elongation factor (P-TEFb), the bromodomain-containing protein (BRD4), and the elongin-like eleven nineteen lysine-rich leukemia protein (ELL). However, transcription at many of these rapidly induced genes is transient, wherein swift departure of P-TEFb, BRD4, and ELL coincides with termination of transcriptional elongation. Unexpectedly, both p300 and pol II remain accumulated or "bookmarked" at the proximal promoter long after transcription has terminated, demarking a clear mechanistic separation between the recruitment and elongation phases of transcription in vivo. The bookmarked pol II is depleted of both serine-2 and serine-5 phosphorylation of its C-terminal domain and remains proximally positioned at the promoter for hours. Surprisingly, these p300/pol II bookmarked genes can be readily reactivated, and elongation factors can be reassembled by subsequent addition of nonmitogenic agents that, alone, have minimal effects on transcription in the absence of prior preconditioning by mitogen stimulation. These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development through the dynamic priming of genes for response to rechallenge by secondary stimuli.Fil: Byun, Jung S.. National Cancer Institute; Estados UnidosFil: Wong, Madeline M.. National Cancer Institute; Estados UnidosFil: Cui, Wenwu. National Cancer Institute; Estados UnidosFil: Idelman, Gila. National Cancer Institute; Estados UnidosFil: Li, Quentin. National Cancer Institute; Estados UnidosFil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bilke, Sven. National Cancer Institute; Estados UnidosFil: Haggerty, Cynthia M.. National Cancer Institute; Estados UnidosFil: Player, Audrey. National Cancer Institute; Estados UnidosFil: Wang, Yong Hong. National Cancer Institute; Estados UnidosFil: Thirman, Michael J.. The University Of Chicago Medicine; Estados UnidosFil: Kaberlein, Joseph J.. The University Of Chicago Medicine; Estados UnidosFil: Petrovas, Constantinos. National Institutes of Health; Estados UnidosFil: Koup, Richard A.. National Institutes of Health; Estados UnidosFil: Longo, Dan L.. National Institute On Aging; Estados UnidosFil: Ozato, Keiko. National Instituto of Child Health & Human Development; Estados UnidosFil: Gardner, Kevin. National Cancer Institute; Estados UnidosNational Academy of Sciences2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83950Byun, Jung S.; Wong, Madeline M.; Cui, Wenwu; Idelman, Gila; Li, Quentin; et al.; Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 106; 46; 11-2009; 19286-192910027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0905469106info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/106/46/19286info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:52Zoai:ri.conicet.gov.ar:11336/83950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:52.308CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
title Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
spellingShingle Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
Byun, Jung S.
Ell
Epigenetics
Gene Regulation
Histone Acetylation
Transcription
title_short Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
title_full Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
title_fullStr Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
title_full_unstemmed Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
title_sort Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
dc.creator.none.fl_str_mv Byun, Jung S.
Wong, Madeline M.
Cui, Wenwu
Idelman, Gila
Li, Quentin
de Siervi, Adriana
Bilke, Sven
Haggerty, Cynthia M.
Player, Audrey
Wang, Yong Hong
Thirman, Michael J.
Kaberlein, Joseph J.
Petrovas, Constantinos
Koup, Richard A.
Longo, Dan L.
Ozato, Keiko
Gardner, Kevin
author Byun, Jung S.
author_facet Byun, Jung S.
Wong, Madeline M.
Cui, Wenwu
Idelman, Gila
Li, Quentin
de Siervi, Adriana
Bilke, Sven
Haggerty, Cynthia M.
Player, Audrey
Wang, Yong Hong
Thirman, Michael J.
Kaberlein, Joseph J.
Petrovas, Constantinos
Koup, Richard A.
Longo, Dan L.
Ozato, Keiko
Gardner, Kevin
author_role author
author2 Wong, Madeline M.
Cui, Wenwu
Idelman, Gila
Li, Quentin
de Siervi, Adriana
Bilke, Sven
Haggerty, Cynthia M.
Player, Audrey
Wang, Yong Hong
Thirman, Michael J.
Kaberlein, Joseph J.
Petrovas, Constantinos
Koup, Richard A.
Longo, Dan L.
Ozato, Keiko
Gardner, Kevin
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ell
Epigenetics
Gene Regulation
Histone Acetylation
Transcription
topic Ell
Epigenetics
Gene Regulation
Histone Acetylation
Transcription
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the control of primary genetic responses. The recruitment of p300 and pol II rapidly transitions to the assembly of several elongation factors, including the positive transcriptional elongation factor (P-TEFb), the bromodomain-containing protein (BRD4), and the elongin-like eleven nineteen lysine-rich leukemia protein (ELL). However, transcription at many of these rapidly induced genes is transient, wherein swift departure of P-TEFb, BRD4, and ELL coincides with termination of transcriptional elongation. Unexpectedly, both p300 and pol II remain accumulated or "bookmarked" at the proximal promoter long after transcription has terminated, demarking a clear mechanistic separation between the recruitment and elongation phases of transcription in vivo. The bookmarked pol II is depleted of both serine-2 and serine-5 phosphorylation of its C-terminal domain and remains proximally positioned at the promoter for hours. Surprisingly, these p300/pol II bookmarked genes can be readily reactivated, and elongation factors can be reassembled by subsequent addition of nonmitogenic agents that, alone, have minimal effects on transcription in the absence of prior preconditioning by mitogen stimulation. These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development through the dynamic priming of genes for response to rechallenge by secondary stimuli.
Fil: Byun, Jung S.. National Cancer Institute; Estados Unidos
Fil: Wong, Madeline M.. National Cancer Institute; Estados Unidos
Fil: Cui, Wenwu. National Cancer Institute; Estados Unidos
Fil: Idelman, Gila. National Cancer Institute; Estados Unidos
Fil: Li, Quentin. National Cancer Institute; Estados Unidos
Fil: de Siervi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bilke, Sven. National Cancer Institute; Estados Unidos
Fil: Haggerty, Cynthia M.. National Cancer Institute; Estados Unidos
Fil: Player, Audrey. National Cancer Institute; Estados Unidos
Fil: Wang, Yong Hong. National Cancer Institute; Estados Unidos
Fil: Thirman, Michael J.. The University Of Chicago Medicine; Estados Unidos
Fil: Kaberlein, Joseph J.. The University Of Chicago Medicine; Estados Unidos
Fil: Petrovas, Constantinos. National Institutes of Health; Estados Unidos
Fil: Koup, Richard A.. National Institutes of Health; Estados Unidos
Fil: Longo, Dan L.. National Institute On Aging; Estados Unidos
Fil: Ozato, Keiko. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Gardner, Kevin. National Cancer Institute; Estados Unidos
description Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the control of primary genetic responses. The recruitment of p300 and pol II rapidly transitions to the assembly of several elongation factors, including the positive transcriptional elongation factor (P-TEFb), the bromodomain-containing protein (BRD4), and the elongin-like eleven nineteen lysine-rich leukemia protein (ELL). However, transcription at many of these rapidly induced genes is transient, wherein swift departure of P-TEFb, BRD4, and ELL coincides with termination of transcriptional elongation. Unexpectedly, both p300 and pol II remain accumulated or "bookmarked" at the proximal promoter long after transcription has terminated, demarking a clear mechanistic separation between the recruitment and elongation phases of transcription in vivo. The bookmarked pol II is depleted of both serine-2 and serine-5 phosphorylation of its C-terminal domain and remains proximally positioned at the promoter for hours. Surprisingly, these p300/pol II bookmarked genes can be readily reactivated, and elongation factors can be reassembled by subsequent addition of nonmitogenic agents that, alone, have minimal effects on transcription in the absence of prior preconditioning by mitogen stimulation. These findings suggest that p300 is likely to play an important role in biological processes in which transcriptional bookmarking or preconditioning influences cellular growth and development through the dynamic priming of genes for response to rechallenge by secondary stimuli.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83950
Byun, Jung S.; Wong, Madeline M.; Cui, Wenwu; Idelman, Gila; Li, Quentin; et al.; Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 106; 46; 11-2009; 19286-19291
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83950
identifier_str_mv Byun, Jung S.; Wong, Madeline M.; Cui, Wenwu; Idelman, Gila; Li, Quentin; et al.; Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 106; 46; 11-2009; 19286-19291
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0905469106
info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/106/46/19286
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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