Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease

Autores
Pirola, Carlos Jose; Salatino, Adrian; Fernández Gianotti, Tomas; Castaño, Gustavo Osvaldo; Garaycoechea, Martin Enrique; Sookoian, Silvia Cristina
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The pathogenesis of MASLD (metabolic dysfunction-associated steatotic liver disease), including its severe clinical forms, involves complex processes at all levels of biological organization. This study examined the potential link between the liver microbiome profile and epigenetic factors. Methods: Liver microbial DNA composition was analysed using high throughput 16S rRNA gene sequencing in 116 individuals, with 55% being female, across the spectrum of liver disease severity. Total activity of histone deacetylases (HDACs) and acetyltransferases (HATs) was assayed in nuclear extracts from fresh liver samples. In addition, we measured the global 5-hydroxymethylcytosine (5-hmC) levels of liver DNA. Findings: Patients with MASLD showed a 2.07-fold increase (p = 0.013) in liver total HAT activity. Moreover, a correlation was observed between liver total HAT activity and the score for histological steatosis (Spearman's R = 0.60, p = 1.0E-3) and disease severity (R = 0.40, p = 2.0E-2). Liver HAT and HDAC activities also showed associations with the abundance of several liver bacterial DNAs. Additionally, liver global levels of 5-hmC showed negative correlation with the read number of Bacteroidetes (R = -0.62, p = 9.3E-4) and Gammaproteobacteria (R = -0.43, p = 3.2E-2), while it was positively correlated with the abundance of Acidobacteria (R = 0.42, p = 4.1E-2) and Actinobacteria (R = 0.47, p = 1.8E-2). Interpretation: The host liver epigenome, including the activity of enzymes involved in maintaining the balance between protein acetylation and deacetylation and the global DNA hydroxy-methylation status, may be the target of microbial signals.
Fil: Pirola, Carlos Jose. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salatino, Adrian. Max Planck Institute For Biology Of Ageing.; Alemania
Fil: Fernández Gianotti, Tomas. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides;
Fil: Castaño, Gustavo Osvaldo. Gobierno de la Ciudad de Buenos Aires. Hospital "Dr. Abel Zubizarreta"; Argentina
Fil: Garaycoechea, Martin Enrique. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; Argentina
Fil: Sookoian, Silvia Cristina. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
MASLD
MICROBIOTA
EPIGENETICS
ACETYLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230748
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver diseasePirola, Carlos JoseSalatino, AdrianFernández Gianotti, TomasCastaño, Gustavo OsvaldoGaraycoechea, Martin EnriqueSookoian, Silvia CristinaMASLDMICROBIOTAEPIGENETICSACETYLATIONhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background: The pathogenesis of MASLD (metabolic dysfunction-associated steatotic liver disease), including its severe clinical forms, involves complex processes at all levels of biological organization. This study examined the potential link between the liver microbiome profile and epigenetic factors. Methods: Liver microbial DNA composition was analysed using high throughput 16S rRNA gene sequencing in 116 individuals, with 55% being female, across the spectrum of liver disease severity. Total activity of histone deacetylases (HDACs) and acetyltransferases (HATs) was assayed in nuclear extracts from fresh liver samples. In addition, we measured the global 5-hydroxymethylcytosine (5-hmC) levels of liver DNA. Findings: Patients with MASLD showed a 2.07-fold increase (p = 0.013) in liver total HAT activity. Moreover, a correlation was observed between liver total HAT activity and the score for histological steatosis (Spearman's R = 0.60, p = 1.0E-3) and disease severity (R = 0.40, p = 2.0E-2). Liver HAT and HDAC activities also showed associations with the abundance of several liver bacterial DNAs. Additionally, liver global levels of 5-hmC showed negative correlation with the read number of Bacteroidetes (R = -0.62, p = 9.3E-4) and Gammaproteobacteria (R = -0.43, p = 3.2E-2), while it was positively correlated with the abundance of Acidobacteria (R = 0.42, p = 4.1E-2) and Actinobacteria (R = 0.47, p = 1.8E-2). Interpretation: The host liver epigenome, including the activity of enzymes involved in maintaining the balance between protein acetylation and deacetylation and the global DNA hydroxy-methylation status, may be the target of microbial signals.Fil: Pirola, Carlos Jose. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salatino, Adrian. Max Planck Institute For Biology Of Ageing.; AlemaniaFil: Fernández Gianotti, Tomas. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides;Fil: Castaño, Gustavo Osvaldo. Gobierno de la Ciudad de Buenos Aires. Hospital "Dr. Abel Zubizarreta"; ArgentinaFil: Garaycoechea, Martin Enrique. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; ArgentinaFil: Sookoian, Silvia Cristina. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230748Pirola, Carlos Jose; Salatino, Adrian; Fernández Gianotti, Tomas; Castaño, Gustavo Osvaldo; Garaycoechea, Martin Enrique; et al.; Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease; Elsevier; eBioMedicine; 101; 3-2024; 104996-1050172352-3964CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2352396424000318info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ebiom.2024.104996info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:01Zoai:ri.conicet.gov.ar:11336/230748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:01.744CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
title Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
spellingShingle Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
Pirola, Carlos Jose
MASLD
MICROBIOTA
EPIGENETICS
ACETYLATION
title_short Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
title_full Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
title_fullStr Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
title_full_unstemmed Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
title_sort Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease
dc.creator.none.fl_str_mv Pirola, Carlos Jose
Salatino, Adrian
Fernández Gianotti, Tomas
Castaño, Gustavo Osvaldo
Garaycoechea, Martin Enrique
Sookoian, Silvia Cristina
author Pirola, Carlos Jose
author_facet Pirola, Carlos Jose
Salatino, Adrian
Fernández Gianotti, Tomas
Castaño, Gustavo Osvaldo
Garaycoechea, Martin Enrique
Sookoian, Silvia Cristina
author_role author
author2 Salatino, Adrian
Fernández Gianotti, Tomas
Castaño, Gustavo Osvaldo
Garaycoechea, Martin Enrique
Sookoian, Silvia Cristina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MASLD
MICROBIOTA
EPIGENETICS
ACETYLATION
topic MASLD
MICROBIOTA
EPIGENETICS
ACETYLATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The pathogenesis of MASLD (metabolic dysfunction-associated steatotic liver disease), including its severe clinical forms, involves complex processes at all levels of biological organization. This study examined the potential link between the liver microbiome profile and epigenetic factors. Methods: Liver microbial DNA composition was analysed using high throughput 16S rRNA gene sequencing in 116 individuals, with 55% being female, across the spectrum of liver disease severity. Total activity of histone deacetylases (HDACs) and acetyltransferases (HATs) was assayed in nuclear extracts from fresh liver samples. In addition, we measured the global 5-hydroxymethylcytosine (5-hmC) levels of liver DNA. Findings: Patients with MASLD showed a 2.07-fold increase (p = 0.013) in liver total HAT activity. Moreover, a correlation was observed between liver total HAT activity and the score for histological steatosis (Spearman's R = 0.60, p = 1.0E-3) and disease severity (R = 0.40, p = 2.0E-2). Liver HAT and HDAC activities also showed associations with the abundance of several liver bacterial DNAs. Additionally, liver global levels of 5-hmC showed negative correlation with the read number of Bacteroidetes (R = -0.62, p = 9.3E-4) and Gammaproteobacteria (R = -0.43, p = 3.2E-2), while it was positively correlated with the abundance of Acidobacteria (R = 0.42, p = 4.1E-2) and Actinobacteria (R = 0.47, p = 1.8E-2). Interpretation: The host liver epigenome, including the activity of enzymes involved in maintaining the balance between protein acetylation and deacetylation and the global DNA hydroxy-methylation status, may be the target of microbial signals.
Fil: Pirola, Carlos Jose. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salatino, Adrian. Max Planck Institute For Biology Of Ageing.; Alemania
Fil: Fernández Gianotti, Tomas. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides;
Fil: Castaño, Gustavo Osvaldo. Gobierno de la Ciudad de Buenos Aires. Hospital "Dr. Abel Zubizarreta"; Argentina
Fil: Garaycoechea, Martin Enrique. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; Argentina
Fil: Sookoian, Silvia Cristina. Centro de Investigacion Traslacional En Salud (cenitres) ; Facultad de Cs. de la Salud ; Universidad Maimonides; . Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: The pathogenesis of MASLD (metabolic dysfunction-associated steatotic liver disease), including its severe clinical forms, involves complex processes at all levels of biological organization. This study examined the potential link between the liver microbiome profile and epigenetic factors. Methods: Liver microbial DNA composition was analysed using high throughput 16S rRNA gene sequencing in 116 individuals, with 55% being female, across the spectrum of liver disease severity. Total activity of histone deacetylases (HDACs) and acetyltransferases (HATs) was assayed in nuclear extracts from fresh liver samples. In addition, we measured the global 5-hydroxymethylcytosine (5-hmC) levels of liver DNA. Findings: Patients with MASLD showed a 2.07-fold increase (p = 0.013) in liver total HAT activity. Moreover, a correlation was observed between liver total HAT activity and the score for histological steatosis (Spearman's R = 0.60, p = 1.0E-3) and disease severity (R = 0.40, p = 2.0E-2). Liver HAT and HDAC activities also showed associations with the abundance of several liver bacterial DNAs. Additionally, liver global levels of 5-hmC showed negative correlation with the read number of Bacteroidetes (R = -0.62, p = 9.3E-4) and Gammaproteobacteria (R = -0.43, p = 3.2E-2), while it was positively correlated with the abundance of Acidobacteria (R = 0.42, p = 4.1E-2) and Actinobacteria (R = 0.47, p = 1.8E-2). Interpretation: The host liver epigenome, including the activity of enzymes involved in maintaining the balance between protein acetylation and deacetylation and the global DNA hydroxy-methylation status, may be the target of microbial signals.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230748
Pirola, Carlos Jose; Salatino, Adrian; Fernández Gianotti, Tomas; Castaño, Gustavo Osvaldo; Garaycoechea, Martin Enrique; et al.; Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease; Elsevier; eBioMedicine; 101; 3-2024; 104996-105017
2352-3964
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230748
identifier_str_mv Pirola, Carlos Jose; Salatino, Adrian; Fernández Gianotti, Tomas; Castaño, Gustavo Osvaldo; Garaycoechea, Martin Enrique; et al.; Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease; Elsevier; eBioMedicine; 101; 3-2024; 104996-105017
2352-3964
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2352396424000318
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ebiom.2024.104996
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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