Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina

Autores
Picco, Natalia Yanina; Alustiza, Fabrisio Eduardo; Bellingeri, Romina Valeria; Grosso, Maria Carolina; Motta, Carlos E.; Larriestra, Alejandro J.; Vissio, Claudina; Tiranti, Karina I.; Terzolo, Horacio R.; Moreira, Ana R.; Vivas, Adriana B.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.
Fil: Picco, Natalia Yanina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bellingeri, Romina Valeria. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grosso, Maria Carolina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Motta, Carlos E.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Larriestra, Alejandro J.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Vissio, Claudina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tiranti, Karina I.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Terzolo, Horacio R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moreira, Ana R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vivas, Adriana B.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Materia
ESCHERICHIA COLI
CALVES
DIARRHEA
PCR
VIRULENCE GENES
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9804
Acceder
id CONICETDig_472e796133b51904e164cff972d770b5
oai_identifier_str oai:ri.conicet.gov.ar:11336/9804
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, ArgentinaDetección molecular de cepas patógenas de Escherichia coli aisladas de terneros neonatos de tambo en la provincia de Córdoba, ArgentinaPicco, Natalia YaninaAlustiza, Fabrisio EduardoBellingeri, Romina ValeriaGrosso, Maria CarolinaMotta, Carlos E.Larriestra, Alejandro J.Vissio, ClaudinaTiranti, Karina I.Terzolo, Horacio R.Moreira, Ana R.Vivas, Adriana B.ESCHERICHIA COLICALVESDIARRHEAPCRVIRULENCE GENESARGENTINAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.Fil: Picco, Natalia Yanina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bellingeri, Romina Valeria. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grosso, Maria Carolina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Motta, Carlos E.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; ArgentinaFil: Larriestra, Alejandro J.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; ArgentinaFil: Vissio, Claudina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tiranti, Karina I.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; ArgentinaFil: Terzolo, Horacio R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Moreira, Ana R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Vivas, Adriana B.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaAsociación Argentina de Microbiología2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9804Picco, Natalia Yanina; Alustiza, Fabrisio Eduardo; Bellingeri, Romina Valeria; Grosso, Maria Carolina; Motta, Carlos E.; et al.; Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 2; 6-2015; 95-1020325-75411851-7617enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2015.01.006info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/5k3zzzinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000310info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:30Zoai:ri.conicet.gov.ar:11336/9804instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:30.967CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
Detección molecular de cepas patógenas de Escherichia coli aisladas de terneros neonatos de tambo en la provincia de Córdoba, Argentina
title Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
spellingShingle Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
Picco, Natalia Yanina
ESCHERICHIA COLI
CALVES
DIARRHEA
PCR
VIRULENCE GENES
ARGENTINA
title_short Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_full Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_fullStr Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_full_unstemmed Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_sort Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
dc.creator.none.fl_str_mv Picco, Natalia Yanina
Alustiza, Fabrisio Eduardo
Bellingeri, Romina Valeria
Grosso, Maria Carolina
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
author Picco, Natalia Yanina
author_facet Picco, Natalia Yanina
Alustiza, Fabrisio Eduardo
Bellingeri, Romina Valeria
Grosso, Maria Carolina
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
author_role author
author2 Alustiza, Fabrisio Eduardo
Bellingeri, Romina Valeria
Grosso, Maria Carolina
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESCHERICHIA COLI
CALVES
DIARRHEA
PCR
VIRULENCE GENES
ARGENTINA
topic ESCHERICHIA COLI
CALVES
DIARRHEA
PCR
VIRULENCE GENES
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.
Fil: Picco, Natalia Yanina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bellingeri, Romina Valeria. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grosso, Maria Carolina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Motta, Carlos E.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Larriestra, Alejandro J.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Vissio, Claudina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tiranti, Karina I.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Patologia Animal; Argentina
Fil: Terzolo, Horacio R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moreira, Ana R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vivas, Adriana B.. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
description The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9804
Picco, Natalia Yanina; Alustiza, Fabrisio Eduardo; Bellingeri, Romina Valeria; Grosso, Maria Carolina; Motta, Carlos E.; et al.; Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 2; 6-2015; 95-102
0325-7541
1851-7617
url http://hdl.handle.net/11336/9804
identifier_str_mv Picco, Natalia Yanina; Alustiza, Fabrisio Eduardo; Bellingeri, Romina Valeria; Grosso, Maria Carolina; Motta, Carlos E.; et al.; Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 2; 6-2015; 95-102
0325-7541
1851-7617
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2015.01.006
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/5k3zzz
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268606681841664
score 13.13397

Publicaciones similares