First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina

Autores
Ruiz Posse, Agustina María; Fernandez, Franco Daniel; Reyna, Pablo Gastón; Nome, Claudia; Torrico Ramallo, Ada Karina; Giménez Pecci, M. P.; Rodriguez Pardina, P.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During 2021, sweet corn plants cultivated for human consumption in a 400-ha area in Santa Fe province, Argentina, showed severe viruslike symptoms, including marked dwarfism, plant deformation, nerval or internerval vein yellowing, chlorotic rings, chlorosis along leaf edges, and vein thickening (Figure 1–2). Abundant populations of the corn leafhopper Dalbulus maidis, a vector of several pathogens (Carloni et al., 2013), were also observed (Figure 3). Ten diseased plants collected in Helvecia, Santa Fe (31◦07t’47t’t’S, 60◦08t’48t’t’W) were tested by leaf dip and serology (DAS-ELISA or PTA-ELISA) against: Maize yellow striate virus, Mal de Río Cuarto virus, Spiroplasma kunkelii, Sugarcane mosaic virus (JM isolate) (IPAVE, Córdoba, Argentina), Maize rayado fino virus (MRFV) (provided by Castillo Loayza, UNALM, Perú), Maize chlorotic mottle virus (MCMV), Maize dwarf mosaic virus (BIOREBA, Switzerland), Wheat streak mosaic virus (DSMZ, Germany), Cucumber mosaic virus, High Plains wheat mosaic virus (HPWMoV), Maize streak virus (MSV) and Sugarcanemosaic virus (SCMV) (Agdia, USA). The samples tested by serology were positive for one or more of the following pathogens: HPWMoV,MCMV, MRFV, SCMV or S. kunkelii, and six of them had a mild reaction when tested for MSV (Table 1). Furthermore, electron microscopy detected geminivirus-like particles in sample 4, one of the six samples with a mild reaction for MSV (Figure 4). The DNA of this sample was extracted using the CTABmethod, amplified by rolling circle amplification (Inoue-Nagata et al., 2004) and sequenced using a MinIONMk1B devicewith the Rapid Barcoding kit (Oxford Nanopore Technologies, UK). Raw sequences were filtered using the Filtlong (https://github.com/rrwick/Filtlong) software, and assembled using minimap2/miniasm (https://github.com/ lh3/minimap2). Contigs of Maize striate mosaic virus (MSMV) were identified using BLASTn against a local viral reference database available from NCBI and polished with Medaka v1.2.3 (https://github.com/ nanoporetech/medaka). MSMV has 96.6% amino acid identity in the coat protein withMSV, hence the cross-reaction observed in ELISA. Thirty-seven sequences, including dicot- and monocot-infecting species, were aligned with MAFTT, and a maximum-likelihood phylogenetic tree was constructed in IQ-TREE (http://www.iqtree.org/) with 1000 bootstrap replications. The sequence of the Argentinian MSMV isolate (GenBank Accession No. OP811251) has 2746 bp, shares 99% identity with all the previously described MSMV isolates, and the final tree showed that the Argentinian isolate brancheswith representative sequences of the virus (Figure 5) MSMV was first detected in maize and sugarcane in Brazil (Fontenele et al., 2018, Batista et al., 2021). The high population levels of the leafhopper vector and the effects of MSMV on plant growth and yield in susceptible cultivars (Vilanova et al., 2022) warrant further epidemiological studies to assess the importance of this pathogen in Argentina. To our knowledge, this is the first report of MSMV (Mastrevirus, Geminiviridae) infecting Zeamays in Argentina.
Fil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Nome, Claudia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Giménez Pecci, M. P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Materia
Geminiviridae
Saccharata
Zea mays convar
Nivel de accesibilidad
acceso abierto
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https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
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Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/262972
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network_name_str CONICET Digital (CONICET)
spelling First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in ArgentinaRuiz Posse, Agustina MaríaFernandez, Franco DanielReyna, Pablo GastónNome, ClaudiaTorrico Ramallo, Ada KarinaGiménez Pecci, M. P.Rodriguez Pardina, P.GeminiviridaeSaccharataZea mays convarhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4During 2021, sweet corn plants cultivated for human consumption in a 400-ha area in Santa Fe province, Argentina, showed severe viruslike symptoms, including marked dwarfism, plant deformation, nerval or internerval vein yellowing, chlorotic rings, chlorosis along leaf edges, and vein thickening (Figure 1–2). Abundant populations of the corn leafhopper Dalbulus maidis, a vector of several pathogens (Carloni et al., 2013), were also observed (Figure 3). Ten diseased plants collected in Helvecia, Santa Fe (31◦07t’47t’t’S, 60◦08t’48t’t’W) were tested by leaf dip and serology (DAS-ELISA or PTA-ELISA) against: Maize yellow striate virus, Mal de Río Cuarto virus, Spiroplasma kunkelii, Sugarcane mosaic virus (JM isolate) (IPAVE, Córdoba, Argentina), Maize rayado fino virus (MRFV) (provided by Castillo Loayza, UNALM, Perú), Maize chlorotic mottle virus (MCMV), Maize dwarf mosaic virus (BIOREBA, Switzerland), Wheat streak mosaic virus (DSMZ, Germany), Cucumber mosaic virus, High Plains wheat mosaic virus (HPWMoV), Maize streak virus (MSV) and Sugarcanemosaic virus (SCMV) (Agdia, USA). The samples tested by serology were positive for one or more of the following pathogens: HPWMoV,MCMV, MRFV, SCMV or S. kunkelii, and six of them had a mild reaction when tested for MSV (Table 1). Furthermore, electron microscopy detected geminivirus-like particles in sample 4, one of the six samples with a mild reaction for MSV (Figure 4). The DNA of this sample was extracted using the CTABmethod, amplified by rolling circle amplification (Inoue-Nagata et al., 2004) and sequenced using a MinIONMk1B devicewith the Rapid Barcoding kit (Oxford Nanopore Technologies, UK). Raw sequences were filtered using the Filtlong (https://github.com/rrwick/Filtlong) software, and assembled using minimap2/miniasm (https://github.com/ lh3/minimap2). Contigs of Maize striate mosaic virus (MSMV) were identified using BLASTn against a local viral reference database available from NCBI and polished with Medaka v1.2.3 (https://github.com/ nanoporetech/medaka). MSMV has 96.6% amino acid identity in the coat protein withMSV, hence the cross-reaction observed in ELISA. Thirty-seven sequences, including dicot- and monocot-infecting species, were aligned with MAFTT, and a maximum-likelihood phylogenetic tree was constructed in IQ-TREE (http://www.iqtree.org/) with 1000 bootstrap replications. The sequence of the Argentinian MSMV isolate (GenBank Accession No. OP811251) has 2746 bp, shares 99% identity with all the previously described MSMV isolates, and the final tree showed that the Argentinian isolate brancheswith representative sequences of the virus (Figure 5) MSMV was first detected in maize and sugarcane in Brazil (Fontenele et al., 2018, Batista et al., 2021). The high population levels of the leafhopper vector and the effects of MSMV on plant growth and yield in susceptible cultivars (Vilanova et al., 2022) warrant further epidemiological studies to assess the importance of this pathogen in Argentina. To our knowledge, this is the first report of MSMV (Mastrevirus, Geminiviridae) infecting Zeamays in Argentina.Fil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Reyna, Pablo Gastón. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giménez Pecci, M. P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Rodriguez Pardina, P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaBritish Society for Plant Pathology2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262972Ruiz Posse, Agustina María; Fernandez, Franco Daniel; Reyna, Pablo Gastón; Nome, Claudia; Torrico Ramallo, Ada Karina; et al.; First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina; British Society for Plant Pathology; New Disease Reports; 47; 2; 6-2023; 1-42044-0588CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1002/ndr2.12186info:eu-repo/semantics/altIdentifier/doi/10.1002/ndr2.12186info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:58Zoai:ri.conicet.gov.ar:11336/262972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:58.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
title First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
spellingShingle First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
Ruiz Posse, Agustina María
Geminiviridae
Saccharata
Zea mays convar
title_short First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
title_full First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
title_fullStr First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
title_full_unstemmed First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
title_sort First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina
dc.creator.none.fl_str_mv Ruiz Posse, Agustina María
Fernandez, Franco Daniel
Reyna, Pablo Gastón
Nome, Claudia
Torrico Ramallo, Ada Karina
Giménez Pecci, M. P.
Rodriguez Pardina, P.
author Ruiz Posse, Agustina María
author_facet Ruiz Posse, Agustina María
Fernandez, Franco Daniel
Reyna, Pablo Gastón
Nome, Claudia
Torrico Ramallo, Ada Karina
Giménez Pecci, M. P.
Rodriguez Pardina, P.
author_role author
author2 Fernandez, Franco Daniel
Reyna, Pablo Gastón
Nome, Claudia
Torrico Ramallo, Ada Karina
Giménez Pecci, M. P.
Rodriguez Pardina, P.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Geminiviridae
Saccharata
Zea mays convar
topic Geminiviridae
Saccharata
Zea mays convar
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv During 2021, sweet corn plants cultivated for human consumption in a 400-ha area in Santa Fe province, Argentina, showed severe viruslike symptoms, including marked dwarfism, plant deformation, nerval or internerval vein yellowing, chlorotic rings, chlorosis along leaf edges, and vein thickening (Figure 1–2). Abundant populations of the corn leafhopper Dalbulus maidis, a vector of several pathogens (Carloni et al., 2013), were also observed (Figure 3). Ten diseased plants collected in Helvecia, Santa Fe (31◦07t’47t’t’S, 60◦08t’48t’t’W) were tested by leaf dip and serology (DAS-ELISA or PTA-ELISA) against: Maize yellow striate virus, Mal de Río Cuarto virus, Spiroplasma kunkelii, Sugarcane mosaic virus (JM isolate) (IPAVE, Córdoba, Argentina), Maize rayado fino virus (MRFV) (provided by Castillo Loayza, UNALM, Perú), Maize chlorotic mottle virus (MCMV), Maize dwarf mosaic virus (BIOREBA, Switzerland), Wheat streak mosaic virus (DSMZ, Germany), Cucumber mosaic virus, High Plains wheat mosaic virus (HPWMoV), Maize streak virus (MSV) and Sugarcanemosaic virus (SCMV) (Agdia, USA). The samples tested by serology were positive for one or more of the following pathogens: HPWMoV,MCMV, MRFV, SCMV or S. kunkelii, and six of them had a mild reaction when tested for MSV (Table 1). Furthermore, electron microscopy detected geminivirus-like particles in sample 4, one of the six samples with a mild reaction for MSV (Figure 4). The DNA of this sample was extracted using the CTABmethod, amplified by rolling circle amplification (Inoue-Nagata et al., 2004) and sequenced using a MinIONMk1B devicewith the Rapid Barcoding kit (Oxford Nanopore Technologies, UK). Raw sequences were filtered using the Filtlong (https://github.com/rrwick/Filtlong) software, and assembled using minimap2/miniasm (https://github.com/ lh3/minimap2). Contigs of Maize striate mosaic virus (MSMV) were identified using BLASTn against a local viral reference database available from NCBI and polished with Medaka v1.2.3 (https://github.com/ nanoporetech/medaka). MSMV has 96.6% amino acid identity in the coat protein withMSV, hence the cross-reaction observed in ELISA. Thirty-seven sequences, including dicot- and monocot-infecting species, were aligned with MAFTT, and a maximum-likelihood phylogenetic tree was constructed in IQ-TREE (http://www.iqtree.org/) with 1000 bootstrap replications. The sequence of the Argentinian MSMV isolate (GenBank Accession No. OP811251) has 2746 bp, shares 99% identity with all the previously described MSMV isolates, and the final tree showed that the Argentinian isolate brancheswith representative sequences of the virus (Figure 5) MSMV was first detected in maize and sugarcane in Brazil (Fontenele et al., 2018, Batista et al., 2021). The high population levels of the leafhopper vector and the effects of MSMV on plant growth and yield in susceptible cultivars (Vilanova et al., 2022) warrant further epidemiological studies to assess the importance of this pathogen in Argentina. To our knowledge, this is the first report of MSMV (Mastrevirus, Geminiviridae) infecting Zeamays in Argentina.
Fil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Nome, Claudia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Giménez Pecci, M. P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, P.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
description During 2021, sweet corn plants cultivated for human consumption in a 400-ha area in Santa Fe province, Argentina, showed severe viruslike symptoms, including marked dwarfism, plant deformation, nerval or internerval vein yellowing, chlorotic rings, chlorosis along leaf edges, and vein thickening (Figure 1–2). Abundant populations of the corn leafhopper Dalbulus maidis, a vector of several pathogens (Carloni et al., 2013), were also observed (Figure 3). Ten diseased plants collected in Helvecia, Santa Fe (31◦07t’47t’t’S, 60◦08t’48t’t’W) were tested by leaf dip and serology (DAS-ELISA or PTA-ELISA) against: Maize yellow striate virus, Mal de Río Cuarto virus, Spiroplasma kunkelii, Sugarcane mosaic virus (JM isolate) (IPAVE, Córdoba, Argentina), Maize rayado fino virus (MRFV) (provided by Castillo Loayza, UNALM, Perú), Maize chlorotic mottle virus (MCMV), Maize dwarf mosaic virus (BIOREBA, Switzerland), Wheat streak mosaic virus (DSMZ, Germany), Cucumber mosaic virus, High Plains wheat mosaic virus (HPWMoV), Maize streak virus (MSV) and Sugarcanemosaic virus (SCMV) (Agdia, USA). The samples tested by serology were positive for one or more of the following pathogens: HPWMoV,MCMV, MRFV, SCMV or S. kunkelii, and six of them had a mild reaction when tested for MSV (Table 1). Furthermore, electron microscopy detected geminivirus-like particles in sample 4, one of the six samples with a mild reaction for MSV (Figure 4). The DNA of this sample was extracted using the CTABmethod, amplified by rolling circle amplification (Inoue-Nagata et al., 2004) and sequenced using a MinIONMk1B devicewith the Rapid Barcoding kit (Oxford Nanopore Technologies, UK). Raw sequences were filtered using the Filtlong (https://github.com/rrwick/Filtlong) software, and assembled using minimap2/miniasm (https://github.com/ lh3/minimap2). Contigs of Maize striate mosaic virus (MSMV) were identified using BLASTn against a local viral reference database available from NCBI and polished with Medaka v1.2.3 (https://github.com/ nanoporetech/medaka). MSMV has 96.6% amino acid identity in the coat protein withMSV, hence the cross-reaction observed in ELISA. Thirty-seven sequences, including dicot- and monocot-infecting species, were aligned with MAFTT, and a maximum-likelihood phylogenetic tree was constructed in IQ-TREE (http://www.iqtree.org/) with 1000 bootstrap replications. The sequence of the Argentinian MSMV isolate (GenBank Accession No. OP811251) has 2746 bp, shares 99% identity with all the previously described MSMV isolates, and the final tree showed that the Argentinian isolate brancheswith representative sequences of the virus (Figure 5) MSMV was first detected in maize and sugarcane in Brazil (Fontenele et al., 2018, Batista et al., 2021). The high population levels of the leafhopper vector and the effects of MSMV on plant growth and yield in susceptible cultivars (Vilanova et al., 2022) warrant further epidemiological studies to assess the importance of this pathogen in Argentina. To our knowledge, this is the first report of MSMV (Mastrevirus, Geminiviridae) infecting Zeamays in Argentina.
publishDate 2023
dc.date.none.fl_str_mv 2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262972
Ruiz Posse, Agustina María; Fernandez, Franco Daniel; Reyna, Pablo Gastón; Nome, Claudia; Torrico Ramallo, Ada Karina; et al.; First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina; British Society for Plant Pathology; New Disease Reports; 47; 2; 6-2023; 1-4
2044-0588
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262972
identifier_str_mv Ruiz Posse, Agustina María; Fernandez, Franco Daniel; Reyna, Pablo Gastón; Nome, Claudia; Torrico Ramallo, Ada Karina; et al.; First report of Maize striate mosaic virus, a mastrevirus infecting Zea mays in Argentina; British Society for Plant Pathology; New Disease Reports; 47; 2; 6-2023; 1-4
2044-0588
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1002/ndr2.12186
info:eu-repo/semantics/altIdentifier/doi/10.1002/ndr2.12186
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv British Society for Plant Pathology
publisher.none.fl_str_mv British Society for Plant Pathology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613086065983488
score 13.070432

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