Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C

Autores
Alcain, Julieta María; Podaza, Enrique Arturo; Gori, María Soledad; Salamone, Gabriela Veronica; Vermeulen, Elba Monica
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway.
Fil: Alcain, Julieta María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Gori, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
DENDRITIC CELLS
HISTAMINE
PROTEIN KINASE C
CYTOTOXIC T LYMPHOCYTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40967
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40967
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase CAlcain, Julieta MaríaPodaza, Enrique ArturoGori, María SoledadSalamone, Gabriela VeronicaVermeulen, Elba MonicaDENDRITIC CELLSHISTAMINEPROTEIN KINASE CCYTOTOXIC T LYMPHOCYTEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway.Fil: Alcain, Julieta María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Gori, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaHindawi Publishing Corporation2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40967Alcain, Julieta María; Podaza, Enrique Arturo; Gori, María Soledad; Salamone, Gabriela Veronica; Vermeulen, Elba Monica; Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C; Hindawi Publishing Corporation; Mediators of Inflammation; 2017; 7-2017; 1-120962-9351CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/mi/2017/9402814/info:eu-repo/semantics/altIdentifier/doi/10.1155/2017/9402814info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:26:55Zoai:ri.conicet.gov.ar:11336/40967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:26:55.751CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
title Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
spellingShingle Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
Alcain, Julieta María
DENDRITIC CELLS
HISTAMINE
PROTEIN KINASE C
CYTOTOXIC T LYMPHOCYTES
title_short Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
title_full Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
title_fullStr Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
title_full_unstemmed Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
title_sort Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C
dc.creator.none.fl_str_mv Alcain, Julieta María
Podaza, Enrique Arturo
Gori, María Soledad
Salamone, Gabriela Veronica
Vermeulen, Elba Monica
author Alcain, Julieta María
author_facet Alcain, Julieta María
Podaza, Enrique Arturo
Gori, María Soledad
Salamone, Gabriela Veronica
Vermeulen, Elba Monica
author_role author
author2 Podaza, Enrique Arturo
Gori, María Soledad
Salamone, Gabriela Veronica
Vermeulen, Elba Monica
author2_role author
author
author
author
dc.subject.none.fl_str_mv DENDRITIC CELLS
HISTAMINE
PROTEIN KINASE C
CYTOTOXIC T LYMPHOCYTES
topic DENDRITIC CELLS
HISTAMINE
PROTEIN KINASE C
CYTOTOXIC T LYMPHOCYTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway.
Fil: Alcain, Julieta María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Podaza, Enrique Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Gori, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40967
Alcain, Julieta María; Podaza, Enrique Arturo; Gori, María Soledad; Salamone, Gabriela Veronica; Vermeulen, Elba Monica; Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C; Hindawi Publishing Corporation; Mediators of Inflammation; 2017; 7-2017; 1-12
0962-9351
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40967
identifier_str_mv Alcain, Julieta María; Podaza, Enrique Arturo; Gori, María Soledad; Salamone, Gabriela Veronica; Vermeulen, Elba Monica; Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C; Hindawi Publishing Corporation; Mediators of Inflammation; 2017; 7-2017; 1-12
0962-9351
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/mi/2017/9402814/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2017/9402814
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083411505053696
score 13.22299

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