Transfection of Capsaspora owczarzaki, a close unicellular relative of animals

Autores
Parra Acero, Helena; Ros Rocher, Núria; Perez Posada, Alberto; Kożyczkowska, Aleksandra; Sánchez Pons, Núria; Nakata, Azusa; Suga, Hiroshi; Najle, Sebastián Rodrigo; Ruiz Trillo, Iñaki
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.
Fil: Parra Acero, Helena. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Ros Rocher, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Perez Posada, Alberto. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Kożyczkowska, Aleksandra. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Sánchez Pons, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Nakata, Azusa. Prefectural University of Hiroshima; Japón
Fil: Suga, Hiroshi. Prefectural University of Hiroshima; Japón
Fil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; España
Materia
CAPSASPORA OWCZARZAKI
HOLOZOA
MULTICELLULARITY
ORIGIN OF METAZOA
TRANSFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90530
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transfection of Capsaspora owczarzaki, a close unicellular relative of animalsParra Acero, HelenaRos Rocher, NúriaPerez Posada, AlbertoKożyczkowska, AleksandraSánchez Pons, NúriaNakata, AzusaSuga, HiroshiNajle, Sebastián RodrigoRuiz Trillo, IñakiCAPSASPORA OWCZARZAKIHOLOZOAMULTICELLULARITYORIGIN OF METAZOATRANSFECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.Fil: Parra Acero, Helena. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; EspañaFil: Ros Rocher, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; EspañaFil: Perez Posada, Alberto. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; EspañaFil: Kożyczkowska, Aleksandra. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; EspañaFil: Sánchez Pons, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; EspañaFil: Nakata, Azusa. Prefectural University of Hiroshima; JapónFil: Suga, Hiroshi. Prefectural University of Hiroshima; JapónFil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; EspañaCompany of Biologists2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90530Parra Acero, Helena; Ros Rocher, Núria; Perez Posada, Alberto; Kożyczkowska, Aleksandra; Sánchez Pons, Núria; et al.; Transfection of Capsaspora owczarzaki, a close unicellular relative of animals; Company of Biologists; Development; 145; 10; 5-2018; 1-270950-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/lookup/doi/10.1242/dev.162107info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.162107info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:24Zoai:ri.conicet.gov.ar:11336/90530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:25.008CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
spellingShingle Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
Parra Acero, Helena
CAPSASPORA OWCZARZAKI
HOLOZOA
MULTICELLULARITY
ORIGIN OF METAZOA
TRANSFECTION
title_short Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_full Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_fullStr Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_full_unstemmed Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_sort Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
dc.creator.none.fl_str_mv Parra Acero, Helena
Ros Rocher, Núria
Perez Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián Rodrigo
Ruiz Trillo, Iñaki
author Parra Acero, Helena
author_facet Parra Acero, Helena
Ros Rocher, Núria
Perez Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián Rodrigo
Ruiz Trillo, Iñaki
author_role author
author2 Ros Rocher, Núria
Perez Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián Rodrigo
Ruiz Trillo, Iñaki
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAPSASPORA OWCZARZAKI
HOLOZOA
MULTICELLULARITY
ORIGIN OF METAZOA
TRANSFECTION
topic CAPSASPORA OWCZARZAKI
HOLOZOA
MULTICELLULARITY
ORIGIN OF METAZOA
TRANSFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.
Fil: Parra Acero, Helena. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Ros Rocher, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Perez Posada, Alberto. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Kożyczkowska, Aleksandra. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Sánchez Pons, Núria. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España
Fil: Nakata, Azusa. Prefectural University of Hiroshima; Japón
Fil: Suga, Hiroshi. Prefectural University of Hiroshima; Japón
Fil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales. Departamento de Biodiversidad y Biología Evolutiva; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; España
description How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90530
Parra Acero, Helena; Ros Rocher, Núria; Perez Posada, Alberto; Kożyczkowska, Aleksandra; Sánchez Pons, Núria; et al.; Transfection of Capsaspora owczarzaki, a close unicellular relative of animals; Company of Biologists; Development; 145; 10; 5-2018; 1-27
0950-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90530
identifier_str_mv Parra Acero, Helena; Ros Rocher, Núria; Perez Posada, Alberto; Kożyczkowska, Aleksandra; Sánchez Pons, Núria; et al.; Transfection of Capsaspora owczarzaki, a close unicellular relative of animals; Company of Biologists; Development; 145; 10; 5-2018; 1-27
0950-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/lookup/doi/10.1242/dev.162107
info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.162107
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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