Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina

Autores
Arroyo, Alicia S.; López-Escardó, David; Kim, Eunsoo; Ruiz Trillo, Iñaki; Najle, Sebastián Rodrigo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Opisthokonta represents a major lineage of eukaryotes and includes fungi and metazoans, as well as other less known unicellular groups. The latter are paraphyletic assemblages that branch in between the former two groups, and thus are important for understanding the origin and early diversification of opisthokonts. The full range of their diversity, however, has not yet been explored from diverse ecological habitats. Freshwater environments are crucial sources for new diversity; they are considered even more heterogeneous than marine ecosystems. This heterogeneity implies more ecological niches where local eukaryotic communities are located. However, knowledge of the unicellular opisthokont diversity is scarce from freshwater environments. Here, we performed an 18S rDNA metabarcoding study in the Middle Paraná River, Argentina, to characterize the molecular diversity of microbial eukaryotes, in particular unicellular members of Opisthokonta. We identified a potential novel clade branching as a sister-group to Fungi. We also detected in our data that more than 60% operational taxonomic units classified as unicellular holozoans (animals and relatives) represent new taxa at the species level. Of the remaining, the majority was assigned to the newly described holozoan species, Syssomonas multiformis. Together, our results show that a large hidden diversity of unicellular members of opisthokonts still remain to be uncovered. We also found that the geographical and ecological distribution of several taxa considered exclusive to marine environments is wider than previously thought.
Fil: Arroyo, Alicia S.. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España
Fil: López-Escardó, David. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España
Fil: Kim, Eunsoo. American Museum of Natural History; Estados Unidos
Fil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España
Fil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
EARLY-BRANCHING HOLOMYCOTA
FRESHWATER
METABARCODING
MOLECULAR DIVERSITY
PARANÁ RIVER
UNICELLULAR HOLOZOA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89734
Acceder
id CONICETDig_b764b0e5477f3d80036ccf40768fefe1
oai_identifier_str oai:ri.conicet.gov.ar:11336/89734
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, ArgentinaArroyo, Alicia S.López-Escardó, DavidKim, EunsooRuiz Trillo, IñakiNajle, Sebastián RodrigoEARLY-BRANCHING HOLOMYCOTAFRESHWATERMETABARCODINGMOLECULAR DIVERSITYPARANÁ RIVERUNICELLULAR HOLOZOAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Opisthokonta represents a major lineage of eukaryotes and includes fungi and metazoans, as well as other less known unicellular groups. The latter are paraphyletic assemblages that branch in between the former two groups, and thus are important for understanding the origin and early diversification of opisthokonts. The full range of their diversity, however, has not yet been explored from diverse ecological habitats. Freshwater environments are crucial sources for new diversity; they are considered even more heterogeneous than marine ecosystems. This heterogeneity implies more ecological niches where local eukaryotic communities are located. However, knowledge of the unicellular opisthokont diversity is scarce from freshwater environments. Here, we performed an 18S rDNA metabarcoding study in the Middle Paraná River, Argentina, to characterize the molecular diversity of microbial eukaryotes, in particular unicellular members of Opisthokonta. We identified a potential novel clade branching as a sister-group to Fungi. We also detected in our data that more than 60% operational taxonomic units classified as unicellular holozoans (animals and relatives) represent new taxa at the species level. Of the remaining, the majority was assigned to the newly described holozoan species, Syssomonas multiformis. Together, our results show that a large hidden diversity of unicellular members of opisthokonts still remain to be uncovered. We also found that the geographical and ecological distribution of several taxa considered exclusive to marine environments is wider than previously thought.Fil: Arroyo, Alicia S.. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; EspañaFil: López-Escardó, David. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; EspañaFil: Kim, Eunsoo. American Museum of Natural History; Estados UnidosFil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; EspañaFil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFrontiers Research Foundation2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89734Arroyo, Alicia S.; López-Escardó, David; Kim, Eunsoo; Ruiz Trillo, Iñaki; Najle, Sebastián Rodrigo; Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina; Frontiers Research Foundation; Frontiers in Ecology and Evolution; 6; JUL; 7-2018; 1-172296-701XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2018.00099info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fevo.2018.00099/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:18Zoai:ri.conicet.gov.ar:11336/89734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:18.511CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
title Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
spellingShingle Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
Arroyo, Alicia S.
EARLY-BRANCHING HOLOMYCOTA
FRESHWATER
METABARCODING
MOLECULAR DIVERSITY
PARANÁ RIVER
UNICELLULAR HOLOZOA
title_short Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
title_full Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
title_fullStr Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
title_full_unstemmed Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
title_sort Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina
dc.creator.none.fl_str_mv Arroyo, Alicia S.
López-Escardó, David
Kim, Eunsoo
Ruiz Trillo, Iñaki
Najle, Sebastián Rodrigo
author Arroyo, Alicia S.
author_facet Arroyo, Alicia S.
López-Escardó, David
Kim, Eunsoo
Ruiz Trillo, Iñaki
Najle, Sebastián Rodrigo
author_role author
author2 López-Escardó, David
Kim, Eunsoo
Ruiz Trillo, Iñaki
Najle, Sebastián Rodrigo
author2_role author
author
author
author
dc.subject.none.fl_str_mv EARLY-BRANCHING HOLOMYCOTA
FRESHWATER
METABARCODING
MOLECULAR DIVERSITY
PARANÁ RIVER
UNICELLULAR HOLOZOA
topic EARLY-BRANCHING HOLOMYCOTA
FRESHWATER
METABARCODING
MOLECULAR DIVERSITY
PARANÁ RIVER
UNICELLULAR HOLOZOA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Opisthokonta represents a major lineage of eukaryotes and includes fungi and metazoans, as well as other less known unicellular groups. The latter are paraphyletic assemblages that branch in between the former two groups, and thus are important for understanding the origin and early diversification of opisthokonts. The full range of their diversity, however, has not yet been explored from diverse ecological habitats. Freshwater environments are crucial sources for new diversity; they are considered even more heterogeneous than marine ecosystems. This heterogeneity implies more ecological niches where local eukaryotic communities are located. However, knowledge of the unicellular opisthokont diversity is scarce from freshwater environments. Here, we performed an 18S rDNA metabarcoding study in the Middle Paraná River, Argentina, to characterize the molecular diversity of microbial eukaryotes, in particular unicellular members of Opisthokonta. We identified a potential novel clade branching as a sister-group to Fungi. We also detected in our data that more than 60% operational taxonomic units classified as unicellular holozoans (animals and relatives) represent new taxa at the species level. Of the remaining, the majority was assigned to the newly described holozoan species, Syssomonas multiformis. Together, our results show that a large hidden diversity of unicellular members of opisthokonts still remain to be uncovered. We also found that the geographical and ecological distribution of several taxa considered exclusive to marine environments is wider than previously thought.
Fil: Arroyo, Alicia S.. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España
Fil: López-Escardó, David. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España
Fil: Kim, Eunsoo. American Museum of Natural History; Estados Unidos
Fil: Ruiz Trillo, Iñaki. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España
Fil: Najle, Sebastián Rodrigo. Consejo Superior de Investigaciones Científicas; España. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Opisthokonta represents a major lineage of eukaryotes and includes fungi and metazoans, as well as other less known unicellular groups. The latter are paraphyletic assemblages that branch in between the former two groups, and thus are important for understanding the origin and early diversification of opisthokonts. The full range of their diversity, however, has not yet been explored from diverse ecological habitats. Freshwater environments are crucial sources for new diversity; they are considered even more heterogeneous than marine ecosystems. This heterogeneity implies more ecological niches where local eukaryotic communities are located. However, knowledge of the unicellular opisthokont diversity is scarce from freshwater environments. Here, we performed an 18S rDNA metabarcoding study in the Middle Paraná River, Argentina, to characterize the molecular diversity of microbial eukaryotes, in particular unicellular members of Opisthokonta. We identified a potential novel clade branching as a sister-group to Fungi. We also detected in our data that more than 60% operational taxonomic units classified as unicellular holozoans (animals and relatives) represent new taxa at the species level. Of the remaining, the majority was assigned to the newly described holozoan species, Syssomonas multiformis. Together, our results show that a large hidden diversity of unicellular members of opisthokonts still remain to be uncovered. We also found that the geographical and ecological distribution of several taxa considered exclusive to marine environments is wider than previously thought.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89734
Arroyo, Alicia S.; López-Escardó, David; Kim, Eunsoo; Ruiz Trillo, Iñaki; Najle, Sebastián Rodrigo; Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina; Frontiers Research Foundation; Frontiers in Ecology and Evolution; 6; JUL; 7-2018; 1-17
2296-701X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89734
identifier_str_mv Arroyo, Alicia S.; López-Escardó, David; Kim, Eunsoo; Ruiz Trillo, Iñaki; Najle, Sebastián Rodrigo; Novel diversity of deeply branching holomycota and unicellular holozoans revealed by metabarcoding in Middle Paraná River, Argentina; Frontiers Research Foundation; Frontiers in Ecology and Evolution; 6; JUL; 7-2018; 1-17
2296-701X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fevo.2018.00099
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fevo.2018.00099/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613304939446272
score 13.070432

Publicaciones similares