Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals

Autores
Najle, Sebastián Rodrigo; Molina, Maria Celeste; Ruiz Trillo, Iñaki; Uttaro, Antonio Domingo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sterols are essential for several physiological processes in most eukaryotes. Sterols regulate membrane homeostasis and participate in different signalling pathways not only as precursors of steroid hormones and vitamins, but also through its role in the formation of lipid rafts. Two major types of sterols, cholesterol and ergosterol, have been described so far in the opisthokonts, the clade that comprise animals, fungi and their unicellular relatives. Cholesterol predominates in derived bilaterians, whereas ergosterol is what generally defines fungi. We here characterize, by a combination of bioinformatic and biochemical analyses, the sterol metabolism in the filasterean Capsaspora owczarzaki, a close unicellular relative of animals that is becoming a model organism. We found that C. owczarzaki sterol metabolism combines enzymatic activities that are usually considered either characteristic of fungi or exclusive to metazoans. Moreover, we observe a differential transcriptional regulation of this metabolism across its life cycle. Thus, C. owczarzaki alternates between synthesizing 7-dehydrocholesterol de novo, which happens at the cystic stage, and the partial conversion-via a novel pathway-of incorporated cholesterol into ergosterol, the characteristic fungal sterol, in the filopodial and aggregative stages.
Fil: Najle, Sebastián Rodrigo. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ruiz Trillo, Iñaki. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; España
Fil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
CAPSASPORA
CHOLESTEROL
ERGOSTEROL
OPISTHOKONTS
STEROL METABOLISM
TRANSCRIPTIONAL REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61030
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61030
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animalsNajle, Sebastián RodrigoMolina, Maria CelesteRuiz Trillo, IñakiUttaro, Antonio DomingoCAPSASPORACHOLESTEROLERGOSTEROLOPISTHOKONTSSTEROL METABOLISMTRANSCRIPTIONAL REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sterols are essential for several physiological processes in most eukaryotes. Sterols regulate membrane homeostasis and participate in different signalling pathways not only as precursors of steroid hormones and vitamins, but also through its role in the formation of lipid rafts. Two major types of sterols, cholesterol and ergosterol, have been described so far in the opisthokonts, the clade that comprise animals, fungi and their unicellular relatives. Cholesterol predominates in derived bilaterians, whereas ergosterol is what generally defines fungi. We here characterize, by a combination of bioinformatic and biochemical analyses, the sterol metabolism in the filasterean Capsaspora owczarzaki, a close unicellular relative of animals that is becoming a model organism. We found that C. owczarzaki sterol metabolism combines enzymatic activities that are usually considered either characteristic of fungi or exclusive to metazoans. Moreover, we observe a differential transcriptional regulation of this metabolism across its life cycle. Thus, C. owczarzaki alternates between synthesizing 7-dehydrocholesterol de novo, which happens at the cystic stage, and the partial conversion-via a novel pathway-of incorporated cholesterol into ergosterol, the characteristic fungal sterol, in the filopodial and aggregative stages.Fil: Najle, Sebastián Rodrigo. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ruiz Trillo, Iñaki. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; EspañaFil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaRoyal Society of London2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61030Najle, Sebastián Rodrigo; Molina, Maria Celeste; Ruiz Trillo, Iñaki; Uttaro, Antonio Domingo; Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals; Royal Society of London; Open Biology; 6; 7; 7-2016; 1-14; 1600292046-2441CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1098/rsob.160029info:eu-repo/semantics/altIdentifier/url/http://rsob.royalsocietypublishing.org/content/6/7/160029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:37:34Zoai:ri.conicet.gov.ar:11336/61030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:37:34.875CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
title Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
spellingShingle Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
Najle, Sebastián Rodrigo
CAPSASPORA
CHOLESTEROL
ERGOSTEROL
OPISTHOKONTS
STEROL METABOLISM
TRANSCRIPTIONAL REGULATION
title_short Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
title_full Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
title_fullStr Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
title_full_unstemmed Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
title_sort Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals
dc.creator.none.fl_str_mv Najle, Sebastián Rodrigo
Molina, Maria Celeste
Ruiz Trillo, Iñaki
Uttaro, Antonio Domingo
author Najle, Sebastián Rodrigo
author_facet Najle, Sebastián Rodrigo
Molina, Maria Celeste
Ruiz Trillo, Iñaki
Uttaro, Antonio Domingo
author_role author
author2 Molina, Maria Celeste
Ruiz Trillo, Iñaki
Uttaro, Antonio Domingo
author2_role author
author
author
dc.subject.none.fl_str_mv CAPSASPORA
CHOLESTEROL
ERGOSTEROL
OPISTHOKONTS
STEROL METABOLISM
TRANSCRIPTIONAL REGULATION
topic CAPSASPORA
CHOLESTEROL
ERGOSTEROL
OPISTHOKONTS
STEROL METABOLISM
TRANSCRIPTIONAL REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sterols are essential for several physiological processes in most eukaryotes. Sterols regulate membrane homeostasis and participate in different signalling pathways not only as precursors of steroid hormones and vitamins, but also through its role in the formation of lipid rafts. Two major types of sterols, cholesterol and ergosterol, have been described so far in the opisthokonts, the clade that comprise animals, fungi and their unicellular relatives. Cholesterol predominates in derived bilaterians, whereas ergosterol is what generally defines fungi. We here characterize, by a combination of bioinformatic and biochemical analyses, the sterol metabolism in the filasterean Capsaspora owczarzaki, a close unicellular relative of animals that is becoming a model organism. We found that C. owczarzaki sterol metabolism combines enzymatic activities that are usually considered either characteristic of fungi or exclusive to metazoans. Moreover, we observe a differential transcriptional regulation of this metabolism across its life cycle. Thus, C. owczarzaki alternates between synthesizing 7-dehydrocholesterol de novo, which happens at the cystic stage, and the partial conversion-via a novel pathway-of incorporated cholesterol into ergosterol, the characteristic fungal sterol, in the filopodial and aggregative stages.
Fil: Najle, Sebastián Rodrigo. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ruiz Trillo, Iñaki. Universitat Pompeu Fabra; España. Consejo Superior de Investigaciones Científicas; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad de Barcelona; España
Fil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Sterols are essential for several physiological processes in most eukaryotes. Sterols regulate membrane homeostasis and participate in different signalling pathways not only as precursors of steroid hormones and vitamins, but also through its role in the formation of lipid rafts. Two major types of sterols, cholesterol and ergosterol, have been described so far in the opisthokonts, the clade that comprise animals, fungi and their unicellular relatives. Cholesterol predominates in derived bilaterians, whereas ergosterol is what generally defines fungi. We here characterize, by a combination of bioinformatic and biochemical analyses, the sterol metabolism in the filasterean Capsaspora owczarzaki, a close unicellular relative of animals that is becoming a model organism. We found that C. owczarzaki sterol metabolism combines enzymatic activities that are usually considered either characteristic of fungi or exclusive to metazoans. Moreover, we observe a differential transcriptional regulation of this metabolism across its life cycle. Thus, C. owczarzaki alternates between synthesizing 7-dehydrocholesterol de novo, which happens at the cystic stage, and the partial conversion-via a novel pathway-of incorporated cholesterol into ergosterol, the characteristic fungal sterol, in the filopodial and aggregative stages.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61030
Najle, Sebastián Rodrigo; Molina, Maria Celeste; Ruiz Trillo, Iñaki; Uttaro, Antonio Domingo; Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals; Royal Society of London; Open Biology; 6; 7; 7-2016; 1-14; 160029
2046-2441
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61030
identifier_str_mv Najle, Sebastián Rodrigo; Molina, Maria Celeste; Ruiz Trillo, Iñaki; Uttaro, Antonio Domingo; Sterol metabolism in the filasterean Capsaspora owczarzaki has features that resemble both fungi and animals; Royal Society of London; Open Biology; 6; 7; 7-2016; 1-14; 160029
2046-2441
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1098/rsob.160029
info:eu-repo/semantics/altIdentifier/url/http://rsob.royalsocietypublishing.org/content/6/7/160029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of London
publisher.none.fl_str_mv Royal Society of London
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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