Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells

Autores
Cebrón, Julieta Rocío; Bojorge, Mariana Andrea; Marder, Nora Mariel; Miquet, Johanna Gabriela; Gonzalez, Lorena
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Flavonoids were proposed as chemopreventive and chemotherapeutic agents per se or in combination with traditional antitumoral drugs. Epidermal growth factor receptor (EGFR) is associated with tumorigenesis of several tissues and it can be involved in the molecular mechanism of action of several flavonoids. With the aim of investigating if a combinatory therapy involving 2'-nitroflavone (2'NF), a synthetic flavonoid with antitumor properties, and EGFRinhibitors would be a possible effective treatment for breast cancer, we studied if this flavone modulates the expression of EGFR or other receptors that interact or modulate EGFR activity. MDA-MB-231 and MCF-7 breast cancer cells were treated with 2´NF at different concentrations for48 h. Afterwards, the protein content of EGFR, ErbB2, ER alpha, Met and IGF-IR were assessed by immunoblotting. Besides, PARP cleavage and phosphorylation of p38 were determined in the same experimental conditions. Results showed that 2´NF produced a reduction on the content ofErbB2 and IGF-IR in MDA-MB-231 cells, while in MCF-7 cells a decrease on the expression of EGFR, ErbB2 and ER alpha was observed. Both cell lines presented an increment in p38 phosphorylation and PARP cleavage upon 2'NF treatment. In conclusion, 2'NF demonstrated to have effects on the expression of receptors associated with EGFR activity which could justify a possible combinatorytherapy involving 2'NF and EGFR inhibitors.
Fil: Cebrón, Julieta Rocío. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Bojorge, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Miquet, Johanna Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Gonzalez, Lorena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Buenos Aires Breast Cancer Symposium
Argentina
Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias
Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental
Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno"
Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional
Materia
2?-nitroflavone
BREAST CANCER CELLS
EGFR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173533
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/173533
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cellsCebrón, Julieta RocíoBojorge, Mariana AndreaMarder, Nora MarielMiquet, Johanna GabrielaGonzalez, Lorena2?-nitroflavoneBREAST CANCER CELLSEGFRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavonoids were proposed as chemopreventive and chemotherapeutic agents per se or in combination with traditional antitumoral drugs. Epidermal growth factor receptor (EGFR) is associated with tumorigenesis of several tissues and it can be involved in the molecular mechanism of action of several flavonoids. With the aim of investigating if a combinatory therapy involving 2'-nitroflavone (2'NF), a synthetic flavonoid with antitumor properties, and EGFRinhibitors would be a possible effective treatment for breast cancer, we studied if this flavone modulates the expression of EGFR or other receptors that interact or modulate EGFR activity. MDA-MB-231 and MCF-7 breast cancer cells were treated with 2´NF at different concentrations for48 h. Afterwards, the protein content of EGFR, ErbB2, ER alpha, Met and IGF-IR were assessed by immunoblotting. Besides, PARP cleavage and phosphorylation of p38 were determined in the same experimental conditions. Results showed that 2´NF produced a reduction on the content ofErbB2 and IGF-IR in MDA-MB-231 cells, while in MCF-7 cells a decrease on the expression of EGFR, ErbB2 and ER alpha was observed. Both cell lines presented an increment in p38 phosphorylation and PARP cleavage upon 2'NF treatment. In conclusion, 2'NF demonstrated to have effects on the expression of receptors associated with EGFR activity which could justify a possible combinatorytherapy involving 2'NF and EGFR inhibitors.Fil: Cebrón, Julieta Rocío. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Bojorge, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Miquet, Johanna Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gonzalez, Lorena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaBuenos Aires Breast Cancer SymposiumArgentinaUniversidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y NeurocienciasInstituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina ExperimentalCentro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno"Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina TraslacionalFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173533Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells; Buenos Aires Breast Cancer Symposium; Argentina; 2021; 15-161669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/34673503.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:39Zoai:ri.conicet.gov.ar:11336/173533instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:40.04CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
title Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
spellingShingle Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
Cebrón, Julieta Rocío
2?-nitroflavone
BREAST CANCER CELLS
EGFR
title_short Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
title_full Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
title_fullStr Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
title_full_unstemmed Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
title_sort Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
dc.creator.none.fl_str_mv Cebrón, Julieta Rocío
Bojorge, Mariana Andrea
Marder, Nora Mariel
Miquet, Johanna Gabriela
Gonzalez, Lorena
author Cebrón, Julieta Rocío
author_facet Cebrón, Julieta Rocío
Bojorge, Mariana Andrea
Marder, Nora Mariel
Miquet, Johanna Gabriela
Gonzalez, Lorena
author_role author
author2 Bojorge, Mariana Andrea
Marder, Nora Mariel
Miquet, Johanna Gabriela
Gonzalez, Lorena
author2_role author
author
author
author
dc.subject.none.fl_str_mv 2?-nitroflavone
BREAST CANCER CELLS
EGFR
topic 2?-nitroflavone
BREAST CANCER CELLS
EGFR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavonoids were proposed as chemopreventive and chemotherapeutic agents per se or in combination with traditional antitumoral drugs. Epidermal growth factor receptor (EGFR) is associated with tumorigenesis of several tissues and it can be involved in the molecular mechanism of action of several flavonoids. With the aim of investigating if a combinatory therapy involving 2'-nitroflavone (2'NF), a synthetic flavonoid with antitumor properties, and EGFRinhibitors would be a possible effective treatment for breast cancer, we studied if this flavone modulates the expression of EGFR or other receptors that interact or modulate EGFR activity. MDA-MB-231 and MCF-7 breast cancer cells were treated with 2´NF at different concentrations for48 h. Afterwards, the protein content of EGFR, ErbB2, ER alpha, Met and IGF-IR were assessed by immunoblotting. Besides, PARP cleavage and phosphorylation of p38 were determined in the same experimental conditions. Results showed that 2´NF produced a reduction on the content ofErbB2 and IGF-IR in MDA-MB-231 cells, while in MCF-7 cells a decrease on the expression of EGFR, ErbB2 and ER alpha was observed. Both cell lines presented an increment in p38 phosphorylation and PARP cleavage upon 2'NF treatment. In conclusion, 2'NF demonstrated to have effects on the expression of receptors associated with EGFR activity which could justify a possible combinatorytherapy involving 2'NF and EGFR inhibitors.
Fil: Cebrón, Julieta Rocío. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Bojorge, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Miquet, Johanna Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Gonzalez, Lorena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Buenos Aires Breast Cancer Symposium
Argentina
Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias
Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental
Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno"
Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional
description Flavonoids were proposed as chemopreventive and chemotherapeutic agents per se or in combination with traditional antitumoral drugs. Epidermal growth factor receptor (EGFR) is associated with tumorigenesis of several tissues and it can be involved in the molecular mechanism of action of several flavonoids. With the aim of investigating if a combinatory therapy involving 2'-nitroflavone (2'NF), a synthetic flavonoid with antitumor properties, and EGFRinhibitors would be a possible effective treatment for breast cancer, we studied if this flavone modulates the expression of EGFR or other receptors that interact or modulate EGFR activity. MDA-MB-231 and MCF-7 breast cancer cells were treated with 2´NF at different concentrations for48 h. Afterwards, the protein content of EGFR, ErbB2, ER alpha, Met and IGF-IR were assessed by immunoblotting. Besides, PARP cleavage and phosphorylation of p38 were determined in the same experimental conditions. Results showed that 2´NF produced a reduction on the content ofErbB2 and IGF-IR in MDA-MB-231 cells, while in MCF-7 cells a decrease on the expression of EGFR, ErbB2 and ER alpha was observed. Both cell lines presented an increment in p38 phosphorylation and PARP cleavage upon 2'NF treatment. In conclusion, 2'NF demonstrated to have effects on the expression of receptors associated with EGFR activity which could justify a possible combinatorytherapy involving 2'NF and EGFR inhibitors.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173533
Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells; Buenos Aires Breast Cancer Symposium; Argentina; 2021; 15-16
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173533
identifier_str_mv Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells; Buenos Aires Breast Cancer Symposium; Argentina; 2021; 15-16
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/34673503.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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