Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina

Autores
Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Muñoz, Claudio Javier; Combina, Mariana; Toro, Maria Eugenia; Castellanos, Lucia Ines; Vazquez, Fabio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Botrytis cinerea, the causal agent of gray mold, is an important disease of grapes. Yeasts are members of the epiphytic microbial community on surfaces of fruits and vegetables and because some yeasts inhibit fungi they are used as biocontrol agents. The major objective of the present work was to isolate yeasts from grapes, vineyard soil, and grape must and select them for their ability to prevent gray mold onset after harvest. Yeasts that were found effective against the fungus were also assayed for their possible pathogenicity in humans. Two antagonism experiments were performed to study the effect of yeasts on B. cinerea, an in vitro study with Czapeck Yeast Extract Agar and an in vivo study with grape berries at 2 ◦C and 25 ◦C; both experiments were conducted at different yeast concentrations (105, 106 and 107 cfu/mL). Antagonists were subsequently assayed for their ability to colonize and grow in fruit wounds. The biocon-trol yeasts were also examined for their possible pathogenicity in humans: phospholipase and proteolytic activity, growth at 37 ◦C and 42 ◦C, pseudohyphal formation and invasive growth. A total of 225 yeasts belonging to 41 species were isolated from must and grape berries and 65 of them, representing 15 species, exhibited in vitro inhibition of B. cinerea at 25 ◦C. These 65 biocontrol yeasts were subsequently assayed in vivo and 16 of them (15 Saccharomyces cerevisiae and 1 Schizosaccharomyces pombe) showed antagonistic properties against B. cinerea at 25 ◦C. Only one isolate (S. cerevisiae BSc68) was able to inhibit mycelial growth of B. cinerea on grape berries at both 2 ◦C and 25 ◦C. The biomass of this strain in grape wounds increased 221.5-fold at 25 ◦C after 3 d and 325.5-fold at 2 ◦C after 10 d of incubation. An increase in the concentration of certain yeasts significantly enhanced their antagonistic activity. All yeast iso-lates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts. Twelve biocontrol agents (S. cerevisiae) revealed one or more phenotypical characteristics associated with pathogenicity in humans but none of them showed all characteristics together. The fact that there exist few reports on S. cerevisiae and none on Sch. pombe as biocontrol agents against B. cinerea makes our results even more relevant.
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Materia
BIOCONTROL
TABLE GRAPE
SACCHAROMYCES CEREVISIAE
SCHIZOSACCHAROMYCES CEREVISIAE
BOTRYTIS CINEREA
PATHOGENIC YEASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243525
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243525
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in ArgentinaNally, Maria CristinaPesce, Virginia MercedesMaturano, Yolanda PaolaMuñoz, Claudio JavierCombina, MarianaToro, Maria EugeniaCastellanos, Lucia InesVazquez, FabioBIOCONTROLTABLE GRAPESACCHAROMYCES CEREVISIAESCHIZOSACCHAROMYCES CEREVISIAEBOTRYTIS CINEREAPATHOGENIC YEASTShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Botrytis cinerea, the causal agent of gray mold, is an important disease of grapes. Yeasts are members of the epiphytic microbial community on surfaces of fruits and vegetables and because some yeasts inhibit fungi they are used as biocontrol agents. The major objective of the present work was to isolate yeasts from grapes, vineyard soil, and grape must and select them for their ability to prevent gray mold onset after harvest. Yeasts that were found effective against the fungus were also assayed for their possible pathogenicity in humans. Two antagonism experiments were performed to study the effect of yeasts on B. cinerea, an in vitro study with Czapeck Yeast Extract Agar and an in vivo study with grape berries at 2 ◦C and 25 ◦C; both experiments were conducted at different yeast concentrations (105, 106 and 107 cfu/mL). Antagonists were subsequently assayed for their ability to colonize and grow in fruit wounds. The biocon-trol yeasts were also examined for their possible pathogenicity in humans: phospholipase and proteolytic activity, growth at 37 ◦C and 42 ◦C, pseudohyphal formation and invasive growth. A total of 225 yeasts belonging to 41 species were isolated from must and grape berries and 65 of them, representing 15 species, exhibited in vitro inhibition of B. cinerea at 25 ◦C. These 65 biocontrol yeasts were subsequently assayed in vivo and 16 of them (15 Saccharomyces cerevisiae and 1 Schizosaccharomyces pombe) showed antagonistic properties against B. cinerea at 25 ◦C. Only one isolate (S. cerevisiae BSc68) was able to inhibit mycelial growth of B. cinerea on grape berries at both 2 ◦C and 25 ◦C. The biomass of this strain in grape wounds increased 221.5-fold at 25 ◦C after 3 d and 325.5-fold at 2 ◦C after 10 d of incubation. An increase in the concentration of certain yeasts significantly enhanced their antagonistic activity. All yeast iso-lates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts. Twelve biocontrol agents (S. cerevisiae) revealed one or more phenotypical characteristics associated with pathogenicity in humans but none of them showed all characteristics together. The fact that there exist few reports on S. cerevisiae and none on Sch. pombe as biocontrol agents against B. cinerea makes our results even more relevant.Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Castellanos, Lucia Ines. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaElsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243525Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Muñoz, Claudio Javier; Combina, Mariana; et al.; Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina; Elsevier Science; Postharvest Biology and Technology; 64; 1; 2-2012; 40-480925-5214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925521411002237info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2011.09.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:21Zoai:ri.conicet.gov.ar:11336/243525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:22.092CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
title Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
spellingShingle Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
Nally, Maria Cristina
BIOCONTROL
TABLE GRAPE
SACCHAROMYCES CEREVISIAE
SCHIZOSACCHAROMYCES CEREVISIAE
BOTRYTIS CINEREA
PATHOGENIC YEASTS
title_short Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
title_full Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
title_fullStr Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
title_full_unstemmed Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
title_sort Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina
dc.creator.none.fl_str_mv Nally, Maria Cristina
Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Muñoz, Claudio Javier
Combina, Mariana
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author Nally, Maria Cristina
author_facet Nally, Maria Cristina
Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Muñoz, Claudio Javier
Combina, Mariana
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author_role author
author2 Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Muñoz, Claudio Javier
Combina, Mariana
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOCONTROL
TABLE GRAPE
SACCHAROMYCES CEREVISIAE
SCHIZOSACCHAROMYCES CEREVISIAE
BOTRYTIS CINEREA
PATHOGENIC YEASTS
topic BIOCONTROL
TABLE GRAPE
SACCHAROMYCES CEREVISIAE
SCHIZOSACCHAROMYCES CEREVISIAE
BOTRYTIS CINEREA
PATHOGENIC YEASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Botrytis cinerea, the causal agent of gray mold, is an important disease of grapes. Yeasts are members of the epiphytic microbial community on surfaces of fruits and vegetables and because some yeasts inhibit fungi they are used as biocontrol agents. The major objective of the present work was to isolate yeasts from grapes, vineyard soil, and grape must and select them for their ability to prevent gray mold onset after harvest. Yeasts that were found effective against the fungus were also assayed for their possible pathogenicity in humans. Two antagonism experiments were performed to study the effect of yeasts on B. cinerea, an in vitro study with Czapeck Yeast Extract Agar and an in vivo study with grape berries at 2 ◦C and 25 ◦C; both experiments were conducted at different yeast concentrations (105, 106 and 107 cfu/mL). Antagonists were subsequently assayed for their ability to colonize and grow in fruit wounds. The biocon-trol yeasts were also examined for their possible pathogenicity in humans: phospholipase and proteolytic activity, growth at 37 ◦C and 42 ◦C, pseudohyphal formation and invasive growth. A total of 225 yeasts belonging to 41 species were isolated from must and grape berries and 65 of them, representing 15 species, exhibited in vitro inhibition of B. cinerea at 25 ◦C. These 65 biocontrol yeasts were subsequently assayed in vivo and 16 of them (15 Saccharomyces cerevisiae and 1 Schizosaccharomyces pombe) showed antagonistic properties against B. cinerea at 25 ◦C. Only one isolate (S. cerevisiae BSc68) was able to inhibit mycelial growth of B. cinerea on grape berries at both 2 ◦C and 25 ◦C. The biomass of this strain in grape wounds increased 221.5-fold at 25 ◦C after 3 d and 325.5-fold at 2 ◦C after 10 d of incubation. An increase in the concentration of certain yeasts significantly enhanced their antagonistic activity. All yeast iso-lates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts. Twelve biocontrol agents (S. cerevisiae) revealed one or more phenotypical characteristics associated with pathogenicity in humans but none of them showed all characteristics together. The fact that there exist few reports on S. cerevisiae and none on Sch. pombe as biocontrol agents against B. cinerea makes our results even more relevant.
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
description Botrytis cinerea, the causal agent of gray mold, is an important disease of grapes. Yeasts are members of the epiphytic microbial community on surfaces of fruits and vegetables and because some yeasts inhibit fungi they are used as biocontrol agents. The major objective of the present work was to isolate yeasts from grapes, vineyard soil, and grape must and select them for their ability to prevent gray mold onset after harvest. Yeasts that were found effective against the fungus were also assayed for their possible pathogenicity in humans. Two antagonism experiments were performed to study the effect of yeasts on B. cinerea, an in vitro study with Czapeck Yeast Extract Agar and an in vivo study with grape berries at 2 ◦C and 25 ◦C; both experiments were conducted at different yeast concentrations (105, 106 and 107 cfu/mL). Antagonists were subsequently assayed for their ability to colonize and grow in fruit wounds. The biocon-trol yeasts were also examined for their possible pathogenicity in humans: phospholipase and proteolytic activity, growth at 37 ◦C and 42 ◦C, pseudohyphal formation and invasive growth. A total of 225 yeasts belonging to 41 species were isolated from must and grape berries and 65 of them, representing 15 species, exhibited in vitro inhibition of B. cinerea at 25 ◦C. These 65 biocontrol yeasts were subsequently assayed in vivo and 16 of them (15 Saccharomyces cerevisiae and 1 Schizosaccharomyces pombe) showed antagonistic properties against B. cinerea at 25 ◦C. Only one isolate (S. cerevisiae BSc68) was able to inhibit mycelial growth of B. cinerea on grape berries at both 2 ◦C and 25 ◦C. The biomass of this strain in grape wounds increased 221.5-fold at 25 ◦C after 3 d and 325.5-fold at 2 ◦C after 10 d of incubation. An increase in the concentration of certain yeasts significantly enhanced their antagonistic activity. All yeast iso-lates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts. Twelve biocontrol agents (S. cerevisiae) revealed one or more phenotypical characteristics associated with pathogenicity in humans but none of them showed all characteristics together. The fact that there exist few reports on S. cerevisiae and none on Sch. pombe as biocontrol agents against B. cinerea makes our results even more relevant.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243525
Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Muñoz, Claudio Javier; Combina, Mariana; et al.; Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina; Elsevier Science; Postharvest Biology and Technology; 64; 1; 2-2012; 40-48
0925-5214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243525
identifier_str_mv Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Muñoz, Claudio Javier; Combina, Mariana; et al.; Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina; Elsevier Science; Postharvest Biology and Technology; 64; 1; 2-2012; 40-48
0925-5214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2011.09.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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