Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot

Autores
Blanco Ulate, Barbara; Amrine, Katherine C. H.; Collins, Thomas S.; Rivero, Rosa M.; Vicente, Ariel Roberto; Morales Cruz, Abraham; Doyle, Carolyn L.; Ye, Zirou; Allen, Greg; Heymann, Hildegarde; Ebeler, Susan E.; Cantu, Dario
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Noble rot results from exceptional infections of ripe grape (Vitis vinifera) berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and the accumulation of secondary metabolites that enhance wine grape composition. Noble rot-infected berries of cv Sémillon, a white-skinned variety, were collected over 3 years from a commercial vineyard at the same time that fruit were harvested for botrytized wine production. Using an integrated transcriptomics and metabolomics approach, we demonstrate that noble rot alters the metabolism of cv Sémillon berries by inducing biotic and abiotic stress responses as well as ripening processes. During noble rot, B. cinerea induced the expression of key regulators of ripening-associated pathways, some of which are distinctive to the normal ripening of red-skinned cultivars. Enhancement of phenylpropanoid metabolism, characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and red-skinned berry ripening. Transcript and metabolite analyses together with enzymatic assays determined that the biosynthesis of anthocyanins is a consistent hallmark of noble rot in cv Sémillon berries. The biosynthesis of terpenes and fatty acid aroma precursors also increased during noble rot. We finally characterized the impact of noble rot in botrytized wines. Altogether, the results of this work demonstrated that noble rot causes a major reprogramming of berry development and metabolism. This desirable interaction between a fruit and a fungus stimulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of compounds involved in the unique flavor and aroma of botrytized wines.
Fil: Blanco Ulate, Barbara. University of California; Estados Unidos
Fil: Amrine, Katherine C. H.. University of California; Estados Unidos
Fil: Collins, Thomas S.. University of California; Estados Unidos. Washington State University; Estados Unidos
Fil: Rivero, Rosa M.. Consejo Superior de Investigaciones Cientificas; España
Fil: Vicente, Ariel Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Morales Cruz, Abraham. University of California; Estados Unidos
Fil: Doyle, Carolyn L.. University of California; Estados Unidos
Fil: Ye, Zirou. University of California; Estados Unidos
Fil: Allen, Greg. Dolce Winery; Estados Unidos
Fil: Heymann, Hildegarde. University of California; Estados Unidos
Fil: Ebeler, Susan E.. University of California; Estados Unidos
Fil: Cantu, Dario. University of California; Estados Unidos
Materia
Botrytis
Noble Rot
Grape
Physiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10951
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10951
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rotBlanco Ulate, BarbaraAmrine, Katherine C. H.Collins, Thomas S.Rivero, Rosa M.Vicente, Ariel RobertoMorales Cruz, AbrahamDoyle, Carolyn L.Ye, ZirouAllen, GregHeymann, HildegardeEbeler, Susan E.Cantu, DarioBotrytisNoble RotGrapePhysiologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Noble rot results from exceptional infections of ripe grape (Vitis vinifera) berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and the accumulation of secondary metabolites that enhance wine grape composition. Noble rot-infected berries of cv Sémillon, a white-skinned variety, were collected over 3 years from a commercial vineyard at the same time that fruit were harvested for botrytized wine production. Using an integrated transcriptomics and metabolomics approach, we demonstrate that noble rot alters the metabolism of cv Sémillon berries by inducing biotic and abiotic stress responses as well as ripening processes. During noble rot, B. cinerea induced the expression of key regulators of ripening-associated pathways, some of which are distinctive to the normal ripening of red-skinned cultivars. Enhancement of phenylpropanoid metabolism, characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and red-skinned berry ripening. Transcript and metabolite analyses together with enzymatic assays determined that the biosynthesis of anthocyanins is a consistent hallmark of noble rot in cv Sémillon berries. The biosynthesis of terpenes and fatty acid aroma precursors also increased during noble rot. We finally characterized the impact of noble rot in botrytized wines. Altogether, the results of this work demonstrated that noble rot causes a major reprogramming of berry development and metabolism. This desirable interaction between a fruit and a fungus stimulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of compounds involved in the unique flavor and aroma of botrytized wines.Fil: Blanco Ulate, Barbara. University of California; Estados UnidosFil: Amrine, Katherine C. H.. University of California; Estados UnidosFil: Collins, Thomas S.. University of California; Estados Unidos. Washington State University; Estados UnidosFil: Rivero, Rosa M.. Consejo Superior de Investigaciones Cientificas; EspañaFil: Vicente, Ariel Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Morales Cruz, Abraham. University of California; Estados UnidosFil: Doyle, Carolyn L.. University of California; Estados UnidosFil: Ye, Zirou. University of California; Estados UnidosFil: Allen, Greg. Dolce Winery; Estados UnidosFil: Heymann, Hildegarde. University of California; Estados UnidosFil: Ebeler, Susan E.. University of California; Estados UnidosFil: Cantu, Dario. University of California; Estados UnidosAmerican Society Of Plant Biologist2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/10951Blanco Ulate, Barbara; Amrine, Katherine C. H.; Collins, Thomas S.; Rivero, Rosa M.; Vicente, Ariel Roberto; et al.; Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot; American Society Of Plant Biologist; Plant Physiology; 169; 4; 12-2015; 2422-24430032-08891532-2548enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/4/2422.full.pdf+htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:22Zoai:ri.conicet.gov.ar:11336/10951instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:22.979CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
title Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
spellingShingle Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
Blanco Ulate, Barbara
Botrytis
Noble Rot
Grape
Physiology
title_short Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
title_full Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
title_fullStr Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
title_full_unstemmed Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
title_sort Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot
dc.creator.none.fl_str_mv Blanco Ulate, Barbara
Amrine, Katherine C. H.
Collins, Thomas S.
Rivero, Rosa M.
Vicente, Ariel Roberto
Morales Cruz, Abraham
Doyle, Carolyn L.
Ye, Zirou
Allen, Greg
Heymann, Hildegarde
Ebeler, Susan E.
Cantu, Dario
author Blanco Ulate, Barbara
author_facet Blanco Ulate, Barbara
Amrine, Katherine C. H.
Collins, Thomas S.
Rivero, Rosa M.
Vicente, Ariel Roberto
Morales Cruz, Abraham
Doyle, Carolyn L.
Ye, Zirou
Allen, Greg
Heymann, Hildegarde
Ebeler, Susan E.
Cantu, Dario
author_role author
author2 Amrine, Katherine C. H.
Collins, Thomas S.
Rivero, Rosa M.
Vicente, Ariel Roberto
Morales Cruz, Abraham
Doyle, Carolyn L.
Ye, Zirou
Allen, Greg
Heymann, Hildegarde
Ebeler, Susan E.
Cantu, Dario
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Botrytis
Noble Rot
Grape
Physiology
topic Botrytis
Noble Rot
Grape
Physiology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Noble rot results from exceptional infections of ripe grape (Vitis vinifera) berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and the accumulation of secondary metabolites that enhance wine grape composition. Noble rot-infected berries of cv Sémillon, a white-skinned variety, were collected over 3 years from a commercial vineyard at the same time that fruit were harvested for botrytized wine production. Using an integrated transcriptomics and metabolomics approach, we demonstrate that noble rot alters the metabolism of cv Sémillon berries by inducing biotic and abiotic stress responses as well as ripening processes. During noble rot, B. cinerea induced the expression of key regulators of ripening-associated pathways, some of which are distinctive to the normal ripening of red-skinned cultivars. Enhancement of phenylpropanoid metabolism, characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and red-skinned berry ripening. Transcript and metabolite analyses together with enzymatic assays determined that the biosynthesis of anthocyanins is a consistent hallmark of noble rot in cv Sémillon berries. The biosynthesis of terpenes and fatty acid aroma precursors also increased during noble rot. We finally characterized the impact of noble rot in botrytized wines. Altogether, the results of this work demonstrated that noble rot causes a major reprogramming of berry development and metabolism. This desirable interaction between a fruit and a fungus stimulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of compounds involved in the unique flavor and aroma of botrytized wines.
Fil: Blanco Ulate, Barbara. University of California; Estados Unidos
Fil: Amrine, Katherine C. H.. University of California; Estados Unidos
Fil: Collins, Thomas S.. University of California; Estados Unidos. Washington State University; Estados Unidos
Fil: Rivero, Rosa M.. Consejo Superior de Investigaciones Cientificas; España
Fil: Vicente, Ariel Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Morales Cruz, Abraham. University of California; Estados Unidos
Fil: Doyle, Carolyn L.. University of California; Estados Unidos
Fil: Ye, Zirou. University of California; Estados Unidos
Fil: Allen, Greg. Dolce Winery; Estados Unidos
Fil: Heymann, Hildegarde. University of California; Estados Unidos
Fil: Ebeler, Susan E.. University of California; Estados Unidos
Fil: Cantu, Dario. University of California; Estados Unidos
description Noble rot results from exceptional infections of ripe grape (Vitis vinifera) berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and the accumulation of secondary metabolites that enhance wine grape composition. Noble rot-infected berries of cv Sémillon, a white-skinned variety, were collected over 3 years from a commercial vineyard at the same time that fruit were harvested for botrytized wine production. Using an integrated transcriptomics and metabolomics approach, we demonstrate that noble rot alters the metabolism of cv Sémillon berries by inducing biotic and abiotic stress responses as well as ripening processes. During noble rot, B. cinerea induced the expression of key regulators of ripening-associated pathways, some of which are distinctive to the normal ripening of red-skinned cultivars. Enhancement of phenylpropanoid metabolism, characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and red-skinned berry ripening. Transcript and metabolite analyses together with enzymatic assays determined that the biosynthesis of anthocyanins is a consistent hallmark of noble rot in cv Sémillon berries. The biosynthesis of terpenes and fatty acid aroma precursors also increased during noble rot. We finally characterized the impact of noble rot in botrytized wines. Altogether, the results of this work demonstrated that noble rot causes a major reprogramming of berry development and metabolism. This desirable interaction between a fruit and a fungus stimulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of compounds involved in the unique flavor and aroma of botrytized wines.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10951
Blanco Ulate, Barbara; Amrine, Katherine C. H.; Collins, Thomas S.; Rivero, Rosa M.; Vicente, Ariel Roberto; et al.; Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot; American Society Of Plant Biologist; Plant Physiology; 169; 4; 12-2015; 2422-2443
0032-0889
1532-2548
url http://hdl.handle.net/11336/10951
identifier_str_mv Blanco Ulate, Barbara; Amrine, Katherine C. H.; Collins, Thomas S.; Rivero, Rosa M.; Vicente, Ariel Roberto; et al.; Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot; American Society Of Plant Biologist; Plant Physiology; 169; 4; 12-2015; 2422-2443
0032-0889
1532-2548
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/4/2422.full.pdf+html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv American Society Of Plant Biologist
publisher.none.fl_str_mv American Society Of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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