Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model

Autores
Speroni, Lucia; Bustuoabad, Victoria de los Ángeles; Gasparri, Julieta; Chiaramoni, Nadia Silvia; Taira, Maria Cristina; Ruggiero, Raul Alejandro; Alonso, Silvia del Valle
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MC-C fibrosarcoma and B16F0 melanoma tumors were implanted intradermally in the dorsal region of the foot of mice. Tumor progression was compared to standard implantation in the flank. Although foot tumors only reached 13% (MC-C) and 25% (B16F0) of the mean volume of flank tumors, a more malignant phenotype in terms of histology and survival rate was observed in this type of tumors. Moreover, lung metastases were only detected in hosts bearing foot tumors, in contrast to MC-C and B16F0 populations with tumors growing in the flank. In addition, cellular influx and local immune reaction were higher in the dorsal region of the foot. According to our results, the dermis of the flank allows excessive tumor growth due to its low reactivity. Thus, differences in innate and adaptive immune effectors between the evaluated tumor microenvironments would account for the differences in tumor malignancy. Due to its striking differences with the standard flank inoculation, the tumor implantation model herein introduced could be a valuable tool to study the metastatic potential of different cell lines and the microenvironment components affecting tumor growth.
Fil: Speroni, Lucia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustuoabad, Victoria de los Ángeles. Provincia de Buenos Aires. Municipio Malvinas Argentinas. Hospital Municipal Oftalmológico Malvinas Argentina; Argentina
Fil: Gasparri, Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taira, Maria Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Mc-C Fibrosarcoma
B16f0 Melanoma Tumors
Animal Model
Flank And Dorsal Regio Mice Foot
Neoplasm Metastasis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55092
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank modelSperoni, LuciaBustuoabad, Victoria de los ÁngelesGasparri, JulietaChiaramoni, Nadia SilviaTaira, Maria CristinaRuggiero, Raul AlejandroAlonso, Silvia del ValleMc-C FibrosarcomaB16f0 Melanoma TumorsAnimal ModelFlank And Dorsal Regio Mice FootNeoplasm Metastasishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3MC-C fibrosarcoma and B16F0 melanoma tumors were implanted intradermally in the dorsal region of the foot of mice. Tumor progression was compared to standard implantation in the flank. Although foot tumors only reached 13% (MC-C) and 25% (B16F0) of the mean volume of flank tumors, a more malignant phenotype in terms of histology and survival rate was observed in this type of tumors. Moreover, lung metastases were only detected in hosts bearing foot tumors, in contrast to MC-C and B16F0 populations with tumors growing in the flank. In addition, cellular influx and local immune reaction were higher in the dorsal region of the foot. According to our results, the dermis of the flank allows excessive tumor growth due to its low reactivity. Thus, differences in innate and adaptive immune effectors between the evaluated tumor microenvironments would account for the differences in tumor malignancy. Due to its striking differences with the standard flank inoculation, the tumor implantation model herein introduced could be a valuable tool to study the metastatic potential of different cell lines and the microenvironment components affecting tumor growth.Fil: Speroni, Lucia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bustuoabad, Victoria de los Ángeles. Provincia de Buenos Aires. Municipio Malvinas Argentinas. Hospital Municipal Oftalmológico Malvinas Argentina; ArgentinaFil: Gasparri, Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taira, Maria Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; ArgentinaFil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLandes Bioscience2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55092Speroni, Lucia; Bustuoabad, Victoria de los Ángeles; Gasparri, Julieta; Chiaramoni, Nadia Silvia; Taira, Maria Cristina; et al.; Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model; Landes Bioscience; Cancer Biology &Therapy; 8; 4; 12-2009; 375-3791538-40471555-8576CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/cbt.8.4.7452info:eu-repo/semantics/altIdentifier/doi//10.4161/cbt.8.4.7452info:eu-repo/semantics/altIdentifier/pmid/19197148info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:09Zoai:ri.conicet.gov.ar:11336/55092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:10.103CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
title Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
spellingShingle Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
Speroni, Lucia
Mc-C Fibrosarcoma
B16f0 Melanoma Tumors
Animal Model
Flank And Dorsal Regio Mice Foot
Neoplasm Metastasis
title_short Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
title_full Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
title_fullStr Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
title_full_unstemmed Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
title_sort Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model
dc.creator.none.fl_str_mv Speroni, Lucia
Bustuoabad, Victoria de los Ángeles
Gasparri, Julieta
Chiaramoni, Nadia Silvia
Taira, Maria Cristina
Ruggiero, Raul Alejandro
Alonso, Silvia del Valle
author Speroni, Lucia
author_facet Speroni, Lucia
Bustuoabad, Victoria de los Ángeles
Gasparri, Julieta
Chiaramoni, Nadia Silvia
Taira, Maria Cristina
Ruggiero, Raul Alejandro
Alonso, Silvia del Valle
author_role author
author2 Bustuoabad, Victoria de los Ángeles
Gasparri, Julieta
Chiaramoni, Nadia Silvia
Taira, Maria Cristina
Ruggiero, Raul Alejandro
Alonso, Silvia del Valle
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mc-C Fibrosarcoma
B16f0 Melanoma Tumors
Animal Model
Flank And Dorsal Regio Mice Foot
Neoplasm Metastasis
topic Mc-C Fibrosarcoma
B16f0 Melanoma Tumors
Animal Model
Flank And Dorsal Regio Mice Foot
Neoplasm Metastasis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv MC-C fibrosarcoma and B16F0 melanoma tumors were implanted intradermally in the dorsal region of the foot of mice. Tumor progression was compared to standard implantation in the flank. Although foot tumors only reached 13% (MC-C) and 25% (B16F0) of the mean volume of flank tumors, a more malignant phenotype in terms of histology and survival rate was observed in this type of tumors. Moreover, lung metastases were only detected in hosts bearing foot tumors, in contrast to MC-C and B16F0 populations with tumors growing in the flank. In addition, cellular influx and local immune reaction were higher in the dorsal region of the foot. According to our results, the dermis of the flank allows excessive tumor growth due to its low reactivity. Thus, differences in innate and adaptive immune effectors between the evaluated tumor microenvironments would account for the differences in tumor malignancy. Due to its striking differences with the standard flank inoculation, the tumor implantation model herein introduced could be a valuable tool to study the metastatic potential of different cell lines and the microenvironment components affecting tumor growth.
Fil: Speroni, Lucia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustuoabad, Victoria de los Ángeles. Provincia de Buenos Aires. Municipio Malvinas Argentinas. Hospital Municipal Oftalmológico Malvinas Argentina; Argentina
Fil: Gasparri, Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taira, Maria Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description MC-C fibrosarcoma and B16F0 melanoma tumors were implanted intradermally in the dorsal region of the foot of mice. Tumor progression was compared to standard implantation in the flank. Although foot tumors only reached 13% (MC-C) and 25% (B16F0) of the mean volume of flank tumors, a more malignant phenotype in terms of histology and survival rate was observed in this type of tumors. Moreover, lung metastases were only detected in hosts bearing foot tumors, in contrast to MC-C and B16F0 populations with tumors growing in the flank. In addition, cellular influx and local immune reaction were higher in the dorsal region of the foot. According to our results, the dermis of the flank allows excessive tumor growth due to its low reactivity. Thus, differences in innate and adaptive immune effectors between the evaluated tumor microenvironments would account for the differences in tumor malignancy. Due to its striking differences with the standard flank inoculation, the tumor implantation model herein introduced could be a valuable tool to study the metastatic potential of different cell lines and the microenvironment components affecting tumor growth.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55092
Speroni, Lucia; Bustuoabad, Victoria de los Ángeles; Gasparri, Julieta; Chiaramoni, Nadia Silvia; Taira, Maria Cristina; et al.; Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model; Landes Bioscience; Cancer Biology &Therapy; 8; 4; 12-2009; 375-379
1538-4047
1555-8576
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55092
identifier_str_mv Speroni, Lucia; Bustuoabad, Victoria de los Ángeles; Gasparri, Julieta; Chiaramoni, Nadia Silvia; Taira, Maria Cristina; et al.; Alternative site of implantation affects tumor malignancy and metastatic potential in mice: its comparison to the flank model; Landes Bioscience; Cancer Biology &Therapy; 8; 4; 12-2009; 375-379
1538-4047
1555-8576
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/cbt.8.4.7452
info:eu-repo/semantics/altIdentifier/doi//10.4161/cbt.8.4.7452
info:eu-repo/semantics/altIdentifier/pmid/19197148
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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