Symbiotic skin bacteria as a source for sex-specific scents in frogs

Autores
Brunetti, Andrés Eduardo; Lucio Lyra, Mariana; Melo, Weilan G. P.; Andrade, Laura Elena; Palacios Rodríguez, Pablo; Prado, Bárbara M.; Baptista Haddad, Célio Fernando; Tallarico Pupo, Monica; Peporine Lopes, Norberto
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Amphibians are known to possess a wide variety of compounds stored in their skin glands. While significant progress has been made in understanding the chemical diversity and biological relevance of alkaloids, amines, steroids, and peptides, most aspects of the odorous secretions are completely unknown. In this study, we examined sexual variations in the volatile profile from the skin of the tree frog Boana prasina and combined culture and culture-independent methods to investigate if microorganisms might be a source of these compounds. We found that sesquiterpenes, thioethers, and methoxypyrazines are major contributors to the observed sex differences. We also observed that each sex has a distinct profile of methoxypyrazines, and that the chemical origin of these compounds can be traced to a Pseudomonas sp. strain isolated from the frog´s skin. This symbiotic bacterium was present in almost all individuals examined from different sites and was maintained in captive conditions, supporting its significance as the source of methoxypyrazines in these frogs. Our results highlight the potential relevance of bacteria as a source of chemical signals in amphibians and contribute to increasing our understanding of the role that symbiotic associations have in animals.
Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidade de Sao Paulo; Brasil
Fil: Lucio Lyra, Mariana. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Melo, Weilan G. P.. Universidade de Sao Paulo; Brasil
Fil: Andrade, Laura Elena. Universidade de Sao Paulo; Brasil
Fil: Palacios Rodríguez, Pablo. Universidad de los Andes; Colombia
Fil: Prado, Bárbara M.. Universidade de Sao Paulo; Brasil
Fil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Tallarico Pupo, Monica. Universidade de Sao Paulo; Brasil
Fil: Peporine Lopes, Norberto. Universidade de Sao Paulo; Brasil
Materia
AMPHIBIA
ANURA
BACTERIAL COMMUNITY DIVERSITY
CHEMICAL ECOLOGY
SMELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109611
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109611
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Symbiotic skin bacteria as a source for sex-specific scents in frogsBrunetti, Andrés EduardoLucio Lyra, MarianaMelo, Weilan G. P.Andrade, Laura ElenaPalacios Rodríguez, PabloPrado, Bárbara M.Baptista Haddad, Célio FernandoTallarico Pupo, MonicaPeporine Lopes, NorbertoAMPHIBIAANURABACTERIAL COMMUNITY DIVERSITYCHEMICAL ECOLOGYSMELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Amphibians are known to possess a wide variety of compounds stored in their skin glands. While significant progress has been made in understanding the chemical diversity and biological relevance of alkaloids, amines, steroids, and peptides, most aspects of the odorous secretions are completely unknown. In this study, we examined sexual variations in the volatile profile from the skin of the tree frog Boana prasina and combined culture and culture-independent methods to investigate if microorganisms might be a source of these compounds. We found that sesquiterpenes, thioethers, and methoxypyrazines are major contributors to the observed sex differences. We also observed that each sex has a distinct profile of methoxypyrazines, and that the chemical origin of these compounds can be traced to a Pseudomonas sp. strain isolated from the frog´s skin. This symbiotic bacterium was present in almost all individuals examined from different sites and was maintained in captive conditions, supporting its significance as the source of methoxypyrazines in these frogs. Our results highlight the potential relevance of bacteria as a source of chemical signals in amphibians and contribute to increasing our understanding of the role that symbiotic associations have in animals.Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidade de Sao Paulo; BrasilFil: Lucio Lyra, Mariana. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Melo, Weilan G. P.. Universidade de Sao Paulo; BrasilFil: Andrade, Laura Elena. Universidade de Sao Paulo; BrasilFil: Palacios Rodríguez, Pablo. Universidad de los Andes; ColombiaFil: Prado, Bárbara M.. Universidade de Sao Paulo; BrasilFil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Tallarico Pupo, Monica. Universidade de Sao Paulo; BrasilFil: Peporine Lopes, Norberto. Universidade de Sao Paulo; BrasilNational Academy of Sciences2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109611Brunetti, Andrés Eduardo; Lucio Lyra, Mariana; Melo, Weilan G. P.; Andrade, Laura Elena; Palacios Rodríguez, Pablo; et al.; Symbiotic skin bacteria as a source for sex-specific scents in frogs; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 116; 6; 2-2019; 2124-21290027-84241091-6490CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/116/6/2124.shortinfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1806834116info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:59Zoai:ri.conicet.gov.ar:11336/109611instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:59.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Symbiotic skin bacteria as a source for sex-specific scents in frogs
title Symbiotic skin bacteria as a source for sex-specific scents in frogs
spellingShingle Symbiotic skin bacteria as a source for sex-specific scents in frogs
Brunetti, Andrés Eduardo
AMPHIBIA
ANURA
BACTERIAL COMMUNITY DIVERSITY
CHEMICAL ECOLOGY
SMELLS
title_short Symbiotic skin bacteria as a source for sex-specific scents in frogs
title_full Symbiotic skin bacteria as a source for sex-specific scents in frogs
title_fullStr Symbiotic skin bacteria as a source for sex-specific scents in frogs
title_full_unstemmed Symbiotic skin bacteria as a source for sex-specific scents in frogs
title_sort Symbiotic skin bacteria as a source for sex-specific scents in frogs
dc.creator.none.fl_str_mv Brunetti, Andrés Eduardo
Lucio Lyra, Mariana
Melo, Weilan G. P.
Andrade, Laura Elena
Palacios Rodríguez, Pablo
Prado, Bárbara M.
Baptista Haddad, Célio Fernando
Tallarico Pupo, Monica
Peporine Lopes, Norberto
author Brunetti, Andrés Eduardo
author_facet Brunetti, Andrés Eduardo
Lucio Lyra, Mariana
Melo, Weilan G. P.
Andrade, Laura Elena
Palacios Rodríguez, Pablo
Prado, Bárbara M.
Baptista Haddad, Célio Fernando
Tallarico Pupo, Monica
Peporine Lopes, Norberto
author_role author
author2 Lucio Lyra, Mariana
Melo, Weilan G. P.
Andrade, Laura Elena
Palacios Rodríguez, Pablo
Prado, Bárbara M.
Baptista Haddad, Célio Fernando
Tallarico Pupo, Monica
Peporine Lopes, Norberto
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AMPHIBIA
ANURA
BACTERIAL COMMUNITY DIVERSITY
CHEMICAL ECOLOGY
SMELLS
topic AMPHIBIA
ANURA
BACTERIAL COMMUNITY DIVERSITY
CHEMICAL ECOLOGY
SMELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Amphibians are known to possess a wide variety of compounds stored in their skin glands. While significant progress has been made in understanding the chemical diversity and biological relevance of alkaloids, amines, steroids, and peptides, most aspects of the odorous secretions are completely unknown. In this study, we examined sexual variations in the volatile profile from the skin of the tree frog Boana prasina and combined culture and culture-independent methods to investigate if microorganisms might be a source of these compounds. We found that sesquiterpenes, thioethers, and methoxypyrazines are major contributors to the observed sex differences. We also observed that each sex has a distinct profile of methoxypyrazines, and that the chemical origin of these compounds can be traced to a Pseudomonas sp. strain isolated from the frog´s skin. This symbiotic bacterium was present in almost all individuals examined from different sites and was maintained in captive conditions, supporting its significance as the source of methoxypyrazines in these frogs. Our results highlight the potential relevance of bacteria as a source of chemical signals in amphibians and contribute to increasing our understanding of the role that symbiotic associations have in animals.
Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidade de Sao Paulo; Brasil
Fil: Lucio Lyra, Mariana. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Melo, Weilan G. P.. Universidade de Sao Paulo; Brasil
Fil: Andrade, Laura Elena. Universidade de Sao Paulo; Brasil
Fil: Palacios Rodríguez, Pablo. Universidad de los Andes; Colombia
Fil: Prado, Bárbara M.. Universidade de Sao Paulo; Brasil
Fil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Tallarico Pupo, Monica. Universidade de Sao Paulo; Brasil
Fil: Peporine Lopes, Norberto. Universidade de Sao Paulo; Brasil
description Amphibians are known to possess a wide variety of compounds stored in their skin glands. While significant progress has been made in understanding the chemical diversity and biological relevance of alkaloids, amines, steroids, and peptides, most aspects of the odorous secretions are completely unknown. In this study, we examined sexual variations in the volatile profile from the skin of the tree frog Boana prasina and combined culture and culture-independent methods to investigate if microorganisms might be a source of these compounds. We found that sesquiterpenes, thioethers, and methoxypyrazines are major contributors to the observed sex differences. We also observed that each sex has a distinct profile of methoxypyrazines, and that the chemical origin of these compounds can be traced to a Pseudomonas sp. strain isolated from the frog´s skin. This symbiotic bacterium was present in almost all individuals examined from different sites and was maintained in captive conditions, supporting its significance as the source of methoxypyrazines in these frogs. Our results highlight the potential relevance of bacteria as a source of chemical signals in amphibians and contribute to increasing our understanding of the role that symbiotic associations have in animals.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109611
Brunetti, Andrés Eduardo; Lucio Lyra, Mariana; Melo, Weilan G. P.; Andrade, Laura Elena; Palacios Rodríguez, Pablo; et al.; Symbiotic skin bacteria as a source for sex-specific scents in frogs; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 116; 6; 2-2019; 2124-2129
0027-8424
1091-6490
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109611
identifier_str_mv Brunetti, Andrés Eduardo; Lucio Lyra, Mariana; Melo, Weilan G. P.; Andrade, Laura Elena; Palacios Rodríguez, Pablo; et al.; Symbiotic skin bacteria as a source for sex-specific scents in frogs; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 116; 6; 2-2019; 2124-2129
0027-8424
1091-6490
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/116/6/2124.short
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1806834116
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269554544214016
score 13.13397

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