Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs

Autores
Brunetti, Andrés Eduardo; Merib, Josias; Carasek, Eduardo; Caramão, Elina B.; Barbará, Janaina; Zini, Claudia A.; Faivovich, Julián
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents).
Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Merib, Josias. Universidade Federal de Santa Catarina; Brasil
Fil: Carasek, Eduardo. Universidade Federal de Santa Catarina; Brasil
Fil: Caramão, Elina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Barbará, Janaina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zini, Claudia A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Faivovich, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
Chemical Defense
Glands
Skin
Smell
Solid Phase Micro Extraction
Amphibia
Anura
Hylidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46488
Acceder
id CONICETDig_fa2f16c73c99a23beb9cde008f18e021
oai_identifier_str oai:ri.conicet.gov.ar:11336/46488
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas TreefrogsBrunetti, Andrés EduardoMerib, JosiasCarasek, EduardoCaramão, Elina B.Barbará, JanainaZini, Claudia A.Faivovich, JuliánChemical DefenseGlandsSkinSmellSolid Phase Micro ExtractionAmphibiaAnuraHylidaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents).Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Merib, Josias. Universidade Federal de Santa Catarina; BrasilFil: Carasek, Eduardo. Universidade Federal de Santa Catarina; BrasilFil: Caramão, Elina B.. Universidade Federal do Rio Grande do Sul; BrasilFil: Barbará, Janaina. Universidade Federal do Rio Grande do Sul; BrasilFil: Zini, Claudia A.. Universidade Federal do Rio Grande do Sul; BrasilFil: Faivovich, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaSpringer2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46488Brunetti, Andrés Eduardo; Merib, Josias; Carasek, Eduardo; Caramão, Elina B.; Barbará, Janaina; et al.; Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs; Springer; Journal of Chemical Ecology; 41; 4; 4-2015; 360-3720098-03311573-1561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10886-015-0564-zinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10886-015-0564-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:26Zoai:ri.conicet.gov.ar:11336/46488instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:26.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
title Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
spellingShingle Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
Brunetti, Andrés Eduardo
Chemical Defense
Glands
Skin
Smell
Solid Phase Micro Extraction
Amphibia
Anura
Hylidae
title_short Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
title_full Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
title_fullStr Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
title_full_unstemmed Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
title_sort Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
dc.creator.none.fl_str_mv Brunetti, Andrés Eduardo
Merib, Josias
Carasek, Eduardo
Caramão, Elina B.
Barbará, Janaina
Zini, Claudia A.
Faivovich, Julián
author Brunetti, Andrés Eduardo
author_facet Brunetti, Andrés Eduardo
Merib, Josias
Carasek, Eduardo
Caramão, Elina B.
Barbará, Janaina
Zini, Claudia A.
Faivovich, Julián
author_role author
author2 Merib, Josias
Carasek, Eduardo
Caramão, Elina B.
Barbará, Janaina
Zini, Claudia A.
Faivovich, Julián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chemical Defense
Glands
Skin
Smell
Solid Phase Micro Extraction
Amphibia
Anura
Hylidae
topic Chemical Defense
Glands
Skin
Smell
Solid Phase Micro Extraction
Amphibia
Anura
Hylidae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents).
Fil: Brunetti, Andrés Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Merib, Josias. Universidade Federal de Santa Catarina; Brasil
Fil: Carasek, Eduardo. Universidade Federal de Santa Catarina; Brasil
Fil: Caramão, Elina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Barbará, Janaina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zini, Claudia A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Faivovich, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents).
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46488
Brunetti, Andrés Eduardo; Merib, Josias; Carasek, Eduardo; Caramão, Elina B.; Barbará, Janaina; et al.; Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs; Springer; Journal of Chemical Ecology; 41; 4; 4-2015; 360-372
0098-0331
1573-1561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46488
identifier_str_mv Brunetti, Andrés Eduardo; Merib, Josias; Carasek, Eduardo; Caramão, Elina B.; Barbará, Janaina; et al.; Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs; Springer; Journal of Chemical Ecology; 41; 4; 4-2015; 360-372
0098-0331
1573-1561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10886-015-0564-z
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10886-015-0564-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981355903778816
score 12.48226

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