Nitrated fatty acids: synthesis and measurement
- Autores
- Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gelhaus, Stacy L.. University of Pittsburgh; Estados Unidos
Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos - Materia
-
Nitrated Fatty Acid
Mass Spectrometry
Free Radical
Nitration - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/25012
Ver los metadatos del registro completo
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spelling |
Nitrated fatty acids: synthesis and measurementWoodcock, Steven R.Bonacci, Gustavo RobertoGelhaus, Stacy L.Schopfer, Francisco J.Nitrated Fatty AcidMass SpectrometryFree RadicalNitrationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.Fil: Woodcock, Steven R.. University of Pittsburgh; Estados UnidosFil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gelhaus, Stacy L.. University of Pittsburgh; Estados UnidosFil: Schopfer, Francisco J.. University of Pittsburgh; Estados UnidosElsevier Inc2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25012Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-260891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.11.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584912018242info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:59Zoai:ri.conicet.gov.ar:11336/25012instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:59.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Nitrated fatty acids: synthesis and measurement |
title |
Nitrated fatty acids: synthesis and measurement |
spellingShingle |
Nitrated fatty acids: synthesis and measurement Woodcock, Steven R. Nitrated Fatty Acid Mass Spectrometry Free Radical Nitration |
title_short |
Nitrated fatty acids: synthesis and measurement |
title_full |
Nitrated fatty acids: synthesis and measurement |
title_fullStr |
Nitrated fatty acids: synthesis and measurement |
title_full_unstemmed |
Nitrated fatty acids: synthesis and measurement |
title_sort |
Nitrated fatty acids: synthesis and measurement |
dc.creator.none.fl_str_mv |
Woodcock, Steven R. Bonacci, Gustavo Roberto Gelhaus, Stacy L. Schopfer, Francisco J. |
author |
Woodcock, Steven R. |
author_facet |
Woodcock, Steven R. Bonacci, Gustavo Roberto Gelhaus, Stacy L. Schopfer, Francisco J. |
author_role |
author |
author2 |
Bonacci, Gustavo Roberto Gelhaus, Stacy L. Schopfer, Francisco J. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Nitrated Fatty Acid Mass Spectrometry Free Radical Nitration |
topic |
Nitrated Fatty Acid Mass Spectrometry Free Radical Nitration |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed. Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos Fil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gelhaus, Stacy L.. University of Pittsburgh; Estados Unidos Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos |
description |
Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/25012 Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-26 0891-5849 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/25012 |
identifier_str_mv |
Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-26 0891-5849 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.11.015 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584912018242 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Inc |
publisher.none.fl_str_mv |
Elsevier Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614512735420416 |
score |
13.070432 |