Nitrated fatty acids: synthesis and measurement

Autores
Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gelhaus, Stacy L.. University of Pittsburgh; Estados Unidos
Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos
Materia
Nitrated Fatty Acid
Mass Spectrometry
Free Radical
Nitration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25012
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25012
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitrated fatty acids: synthesis and measurementWoodcock, Steven R.Bonacci, Gustavo RobertoGelhaus, Stacy L.Schopfer, Francisco J.Nitrated Fatty AcidMass SpectrometryFree RadicalNitrationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.Fil: Woodcock, Steven R.. University of Pittsburgh; Estados UnidosFil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gelhaus, Stacy L.. University of Pittsburgh; Estados UnidosFil: Schopfer, Francisco J.. University of Pittsburgh; Estados UnidosElsevier Inc2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25012Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-260891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.11.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584912018242info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:21Zoai:ri.conicet.gov.ar:11336/25012instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:22.084CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitrated fatty acids: synthesis and measurement
title Nitrated fatty acids: synthesis and measurement
spellingShingle Nitrated fatty acids: synthesis and measurement
Woodcock, Steven R.
Nitrated Fatty Acid
Mass Spectrometry
Free Radical
Nitration
title_short Nitrated fatty acids: synthesis and measurement
title_full Nitrated fatty acids: synthesis and measurement
title_fullStr Nitrated fatty acids: synthesis and measurement
title_full_unstemmed Nitrated fatty acids: synthesis and measurement
title_sort Nitrated fatty acids: synthesis and measurement
dc.creator.none.fl_str_mv Woodcock, Steven R.
Bonacci, Gustavo Roberto
Gelhaus, Stacy L.
Schopfer, Francisco J.
author Woodcock, Steven R.
author_facet Woodcock, Steven R.
Bonacci, Gustavo Roberto
Gelhaus, Stacy L.
Schopfer, Francisco J.
author_role author
author2 Bonacci, Gustavo Roberto
Gelhaus, Stacy L.
Schopfer, Francisco J.
author2_role author
author
author
dc.subject.none.fl_str_mv Nitrated Fatty Acid
Mass Spectrometry
Free Radical
Nitration
topic Nitrated Fatty Acid
Mass Spectrometry
Free Radical
Nitration
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
Fil: Woodcock, Steven R.. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gelhaus, Stacy L.. University of Pittsburgh; Estados Unidos
Fil: Schopfer, Francisco J.. University of Pittsburgh; Estados Unidos
description Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25012
Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-26
0891-5849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25012
identifier_str_mv Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-26
0891-5849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.11.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584912018242
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782650401947648
score 12.982451

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