Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management

Autores
López, Silvina Marianela Yanil; Pastorino, Graciela N.; Balatti, Pedro Alberto
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión enviada
Descripción
Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.
Materia
Agronomía, reproducción y protección de plantas
Bradyrhizobium
fixation genes
Repetitive sequences
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/8264

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network_name_str CIC Digital (CICBA)
spelling Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage managementLópez, Silvina Marianela YanilPastorino, Graciela N.Balatti, Pedro AlbertoAgronomía, reproducción y protección de plantasBradyrhizobiumfixation genesRepetitive sequencesRhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.2010-06-18info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8264enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/8264Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:03.085CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
title Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
spellingShingle Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
López, Silvina Marianela Yanil
Agronomía, reproducción y protección de plantas
Bradyrhizobium
fixation genes
Repetitive sequences
title_short Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
title_full Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
title_fullStr Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
title_full_unstemmed Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
title_sort Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
dc.creator.none.fl_str_mv López, Silvina Marianela Yanil
Pastorino, Graciela N.
Balatti, Pedro Alberto
author López, Silvina Marianela Yanil
author_facet López, Silvina Marianela Yanil
Pastorino, Graciela N.
Balatti, Pedro Alberto
author_role author
author2 Pastorino, Graciela N.
Balatti, Pedro Alberto
author2_role author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Bradyrhizobium
fixation genes
Repetitive sequences
topic Agronomía, reproducción y protección de plantas
Bradyrhizobium
fixation genes
Repetitive sequences
dc.description.none.fl_txt_mv Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.
description Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-18
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