Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management
- Autores
- López, Silvina Marianela Yanil; Pastorino, Graciela N.; Balatti, Pedro Alberto
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión enviada
- Descripción
- Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.
- Materia
-
Agronomía, reproducción y protección de plantas
Bradyrhizobium
fixation genes
Repetitive sequences - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/8264
Ver los metadatos del registro completo
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Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage managementLópez, Silvina Marianela YanilPastorino, Graciela N.Balatti, Pedro AlbertoAgronomía, reproducción y protección de plantasBradyrhizobiumfixation genesRepetitive sequencesRhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions.2010-06-18info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8264enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/8264Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:03.085CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
title |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
spellingShingle |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management López, Silvina Marianela Yanil Agronomía, reproducción y protección de plantas Bradyrhizobium fixation genes Repetitive sequences |
title_short |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
title_full |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
title_fullStr |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
title_full_unstemmed |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
title_sort |
Structural and regulatory characteristic of Bradyrhizobium japonicum and Bradyrhizobium elkanii isolates recovered from soils cultured with soybean in a zero tillage management |
dc.creator.none.fl_str_mv |
López, Silvina Marianela Yanil Pastorino, Graciela N. Balatti, Pedro Alberto |
author |
López, Silvina Marianela Yanil |
author_facet |
López, Silvina Marianela Yanil Pastorino, Graciela N. Balatti, Pedro Alberto |
author_role |
author |
author2 |
Pastorino, Graciela N. Balatti, Pedro Alberto |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas Bradyrhizobium fixation genes Repetitive sequences |
topic |
Agronomía, reproducción y protección de plantas Bradyrhizobium fixation genes Repetitive sequences |
dc.description.none.fl_txt_mv |
Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions. |
description |
Rhizobia nodulating soybeans were isolated from soils cultivated under zero tillage management for approximately 10 years. The isolates (250) were found to be siblings of strains of B.japonicum and B. elkanii used in commercial inoculants. A subset of 12 of them were selected based upon either their high or low nitrogen fixing activity, compared to control strains E109, Semia 5080, Semia 5079, Semia 587 and Semia 5019, which are the strains used in commercial inoculants in Argentina, Brasil and Uruguay. The similarity of the isolates was assessed by means of BOX, REP-PCR and RSα sequences. Our hypothesis is that differences in the strains ability to fix nitrogen might be due to structural alterations in the two genomic sites containing most of the nitrogen fixing genes or to differential expression of nitrogen fixation genes. We are currently analyzing the structural features of the DNA fragments containing the nitrogen fixation genes. Our preliminary assays consisted in the amplification of a 1169 bp fragment containing nifD that was restricted with EcoRV. We found three different restriction patterns. Also we amplified a 3.5 kb fragment containing RSα, RSβ and nifD that is sequences where mutations might occur at different rates. We are currently setting up amplification reactions aimed at amplifying in four different reactions the nitrogen fixation genes in order to evaluate if the strains lack similarity at this regions. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-18 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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conferenceObject |
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submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/8264 |
url |
https://digital.cic.gba.gob.ar/handle/11746/8264 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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application/pdf application/pdf |
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CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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marisa.degiusti@sedici.unlp.edu.ar |
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