Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation

Autores
Bongiovani, Natalia; Popovich, Cecilia; Martínez, Ana María; Constenla, Diana; Leonardi, Patricia I.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
The large-scale culture of microalgae has become a focus of interest for the biorefinery industry, combining the production of biofuels with that of other value-added bioproducts and thus increasing the profitability of the process. In order to evaluate its potential as biodiesel feedstock under a biorefinery approach, the biomass, pigment, nutrient, total lipid and lipid fraction kinetics of the Argentinian Nannochloropsis oceanica CCALA 978 strain were analysed. The species was first cultivated under optimal conditions (f/2 medium) and then transferred to nutritional stress conditions (N-deprivation or P-deprivation media). Under complete f/2 medium, N. oceanica presented maximum growth rate (μ) and chlorophyll-a values and minimum values for lipid and carotenoid content. The high nutrient consumption rate (N or P) in this exponential phase was associated with increasing cell density. Under both nitrate and phosphate deprivation, μ was drastically reduced but biomass production reached its highest values, indicating a phase of metabolite accumulation. Maximum values of total lipids (ca. 39% dw) and carotenoids (1100 × 10−3 ± 15 μg mL−1) were obtained under nitrate deprivation. In addition, total lipid composition was characterized by 92% neutral lipids, 0.7% phospholipids, a high percentage of C16:1 (ca. 37%of total fatty acids) and minimum values of PUFA (5.9% of total fatty acids). Biodiesel properties inferred from the fatty acid methyl ester profile of neutral lipids meet the standards established by EN 14214 and ASTMD 6751-08. Although N and P deficiency both produced an increase in neutral lipids in the studied strain, under a biorefinery approach, nitrate deprivation is considered to be a better method of stress induction than phosphate deprivation for the purpose of co-producing neutral lipids for biodiesel and value-added pigments.
Materia
Ciencias Biológicas
Ciencias Sociales
marine microalga biomass
Nutrient kinetics
Lipid fractions
Pigments
Biodiesel feedstock
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/10803

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network_name_str CIC Digital (CICBA)
spelling Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate DeprivationBongiovani, NataliaPopovich, CeciliaMartínez, Ana MaríaConstenla, DianaLeonardi, Patricia I.Ciencias BiológicasCiencias Socialesmarine microalga biomassNutrient kineticsLipid fractionsPigmentsBiodiesel feedstockThe large-scale culture of microalgae has become a focus of interest for the biorefinery industry, combining the production of biofuels with that of other value-added bioproducts and thus increasing the profitability of the process. In order to evaluate its potential as biodiesel feedstock under a biorefinery approach, the biomass, pigment, nutrient, total lipid and lipid fraction kinetics of the Argentinian Nannochloropsis oceanica CCALA 978 strain were analysed. The species was first cultivated under optimal conditions (f/2 medium) and then transferred to nutritional stress conditions (N-deprivation or P-deprivation media). Under complete f/2 medium, N. oceanica presented maximum growth rate (μ) and chlorophyll-a values and minimum values for lipid and carotenoid content. The high nutrient consumption rate (N or P) in this exponential phase was associated with increasing cell density. Under both nitrate and phosphate deprivation, μ was drastically reduced but biomass production reached its highest values, indicating a phase of metabolite accumulation. Maximum values of total lipids (ca. 39% dw) and carotenoids (1100 × 10−3 ± 15 μg mL−1) were obtained under nitrate deprivation. In addition, total lipid composition was characterized by 92% neutral lipids, 0.7% phospholipids, a high percentage of C16:1 (ca. 37%of total fatty acids) and minimum values of PUFA (5.9% of total fatty acids). Biodiesel properties inferred from the fatty acid methyl ester profile of neutral lipids meet the standards established by EN 14214 and ASTMD 6751-08. Although N and P deficiency both produced an increase in neutral lipids in the studied strain, under a biorefinery approach, nitrate deprivation is considered to be a better method of stress induction than phosphate deprivation for the purpose of co-producing neutral lipids for biodiesel and value-added pigments.2019-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10803enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12155-019-10045-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:01Zoai:digital.cic.gba.gob.ar:11746/10803Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:01.532CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
title Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
spellingShingle Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
Bongiovani, Natalia
Ciencias Biológicas
Ciencias Sociales
marine microalga biomass
Nutrient kinetics
Lipid fractions
Pigments
Biodiesel feedstock
title_short Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
title_full Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
title_fullStr Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
title_full_unstemmed Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
title_sort Biorefinery Approach from Nannochloropsis oceanica CCALA 978: Neutral Lipid and Carotenoid Co-Production Under Nitrate or Phosphate Deprivation
dc.creator.none.fl_str_mv Bongiovani, Natalia
Popovich, Cecilia
Martínez, Ana María
Constenla, Diana
Leonardi, Patricia I.
author Bongiovani, Natalia
author_facet Bongiovani, Natalia
Popovich, Cecilia
Martínez, Ana María
Constenla, Diana
Leonardi, Patricia I.
author_role author
author2 Popovich, Cecilia
Martínez, Ana María
Constenla, Diana
Leonardi, Patricia I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Biológicas
Ciencias Sociales
marine microalga biomass
Nutrient kinetics
Lipid fractions
Pigments
Biodiesel feedstock
topic Ciencias Biológicas
Ciencias Sociales
marine microalga biomass
Nutrient kinetics
Lipid fractions
Pigments
Biodiesel feedstock
dc.description.none.fl_txt_mv The large-scale culture of microalgae has become a focus of interest for the biorefinery industry, combining the production of biofuels with that of other value-added bioproducts and thus increasing the profitability of the process. In order to evaluate its potential as biodiesel feedstock under a biorefinery approach, the biomass, pigment, nutrient, total lipid and lipid fraction kinetics of the Argentinian Nannochloropsis oceanica CCALA 978 strain were analysed. The species was first cultivated under optimal conditions (f/2 medium) and then transferred to nutritional stress conditions (N-deprivation or P-deprivation media). Under complete f/2 medium, N. oceanica presented maximum growth rate (μ) and chlorophyll-a values and minimum values for lipid and carotenoid content. The high nutrient consumption rate (N or P) in this exponential phase was associated with increasing cell density. Under both nitrate and phosphate deprivation, μ was drastically reduced but biomass production reached its highest values, indicating a phase of metabolite accumulation. Maximum values of total lipids (ca. 39% dw) and carotenoids (1100 × 10−3 ± 15 μg mL−1) were obtained under nitrate deprivation. In addition, total lipid composition was characterized by 92% neutral lipids, 0.7% phospholipids, a high percentage of C16:1 (ca. 37%of total fatty acids) and minimum values of PUFA (5.9% of total fatty acids). Biodiesel properties inferred from the fatty acid methyl ester profile of neutral lipids meet the standards established by EN 14214 and ASTMD 6751-08. Although N and P deficiency both produced an increase in neutral lipids in the studied strain, under a biorefinery approach, nitrate deprivation is considered to be a better method of stress induction than phosphate deprivation for the purpose of co-producing neutral lipids for biodiesel and value-added pigments.
description The large-scale culture of microalgae has become a focus of interest for the biorefinery industry, combining the production of biofuels with that of other value-added bioproducts and thus increasing the profitability of the process. In order to evaluate its potential as biodiesel feedstock under a biorefinery approach, the biomass, pigment, nutrient, total lipid and lipid fraction kinetics of the Argentinian Nannochloropsis oceanica CCALA 978 strain were analysed. The species was first cultivated under optimal conditions (f/2 medium) and then transferred to nutritional stress conditions (N-deprivation or P-deprivation media). Under complete f/2 medium, N. oceanica presented maximum growth rate (μ) and chlorophyll-a values and minimum values for lipid and carotenoid content. The high nutrient consumption rate (N or P) in this exponential phase was associated with increasing cell density. Under both nitrate and phosphate deprivation, μ was drastically reduced but biomass production reached its highest values, indicating a phase of metabolite accumulation. Maximum values of total lipids (ca. 39% dw) and carotenoids (1100 × 10−3 ± 15 μg mL−1) were obtained under nitrate deprivation. In addition, total lipid composition was characterized by 92% neutral lipids, 0.7% phospholipids, a high percentage of C16:1 (ca. 37%of total fatty acids) and minimum values of PUFA (5.9% of total fatty acids). Biodiesel properties inferred from the fatty acid methyl ester profile of neutral lipids meet the standards established by EN 14214 and ASTMD 6751-08. Although N and P deficiency both produced an increase in neutral lipids in the studied strain, under a biorefinery approach, nitrate deprivation is considered to be a better method of stress induction than phosphate deprivation for the purpose of co-producing neutral lipids for biodiesel and value-added pigments.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
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info:ar-repo/semantics/articulo
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status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/10803
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12155-019-10045-2
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eu_rights_str_mv openAccess
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institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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