Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
- Autores
- Alippi, Adriana Mónica; Lopez Ana Claudia; Balatti, Pedro Alberto
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants ofVaccinium corymbossumby means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to beAgrobacteriumsince they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within thevirC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged toAgrobacteriumbv. 1 (n = 33) orAgrobacteriumbv. 2 (n = 31) only fourteen wereAgrobacterium rubi.A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded thatVacciniumplants are particularly susceptible toAgrobacteriumbv. 1,Agrobacteriumbv. 2, and also toAgrobacterium rubi.To our knowledge this is the first survey ofAgrobacteriumaffecting blueberries in Argentina.
- Materia
-
Agronomía, reproducción y protección de plantas
Agrobacterium
CROWN GALL
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/7195
Ver los metadatos del registro completo
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CICBA_3749d136d96e0bf73092df698a35fd74 |
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oai:digital.cic.gba.gob.ar:11746/7195 |
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network_name_str |
CIC Digital (CICBA) |
spelling |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.Alippi, Adriana MónicaLopez Ana ClaudiaBalatti, Pedro AlbertoAgronomía, reproducción y protección de plantasAgrobacteriumCROWN GALLAgrobacterium rubiAgrobacterium tumefaciensAgrobacterium rhizogenesBiovarsVaccinium corymbossumBlueberriesCrown gall diseaseDiversityPCRThe aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina.2012-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/7195enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:42:59Zoai:digital.cic.gba.gob.ar:11746/7195Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:42:59.374CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
title |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
spellingShingle |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. Alippi, Adriana Mónica Agronomía, reproducción y protección de plantas Agrobacterium CROWN GALL Agrobacterium rubi Agrobacterium tumefaciens Agrobacterium rhizogenes Biovars Vaccinium corymbossum Blueberries Crown gall disease Diversity PCR |
title_short |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
title_full |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
title_fullStr |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
title_full_unstemmed |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
title_sort |
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. |
dc.creator.none.fl_str_mv |
Alippi, Adriana Mónica Lopez Ana Claudia Balatti, Pedro Alberto |
author |
Alippi, Adriana Mónica |
author_facet |
Alippi, Adriana Mónica Lopez Ana Claudia Balatti, Pedro Alberto |
author_role |
author |
author2 |
Lopez Ana Claudia Balatti, Pedro Alberto |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas Agrobacterium CROWN GALL Agrobacterium rubi Agrobacterium tumefaciens Agrobacterium rhizogenes Biovars Vaccinium corymbossum Blueberries Crown gall disease Diversity PCR |
topic |
Agronomía, reproducción y protección de plantas Agrobacterium CROWN GALL Agrobacterium rubi Agrobacterium tumefaciens Agrobacterium rhizogenes Biovars Vaccinium corymbossum Blueberries Crown gall disease Diversity PCR |
dc.description.none.fl_txt_mv |
The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina. |
description |
The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/7195 |
url |
https://digital.cic.gba.gob.ar/handle/11746/7195 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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1842340399627108352 |
score |
12.623145 |