Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.

Autores
Alippi, Adriana Mónica; Lopez Ana Claudia; Balatti, Pedro Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants ofVaccinium corymbossumby means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to beAgrobacteriumsince they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within thevirC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged toAgrobacteriumbv. 1 (n = 33) orAgrobacteriumbv. 2 (n = 31) only fourteen wereAgrobacterium rubi.A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded thatVacciniumplants are particularly susceptible toAgrobacteriumbv. 1,Agrobacteriumbv. 2, and also toAgrobacterium rubi.To our knowledge this is the first survey ofAgrobacteriumaffecting blueberries in Argentina.
Materia
Agronomía, reproducción y protección de plantas
Agrobacterium
CROWN GALL
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/7195

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/7195
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.Alippi, Adriana MónicaLopez Ana ClaudiaBalatti, Pedro AlbertoAgronomía, reproducción y protección de plantasAgrobacteriumCROWN GALLAgrobacterium rubiAgrobacterium tumefaciensAgrobacterium rhizogenesBiovarsVaccinium corymbossumBlueberriesCrown gall diseaseDiversityPCRThe aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina.2012-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/7195enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:42:59Zoai:digital.cic.gba.gob.ar:11746/7195Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:42:59.374CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
title Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
spellingShingle Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
Alippi, Adriana Mónica
Agronomía, reproducción y protección de plantas
Agrobacterium
CROWN GALL
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
title_short Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
title_full Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
title_fullStr Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
title_full_unstemmed Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
title_sort Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
Lopez Ana Claudia
Balatti, Pedro Alberto
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
Lopez Ana Claudia
Balatti, Pedro Alberto
author_role author
author2 Lopez Ana Claudia
Balatti, Pedro Alberto
author2_role author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Agrobacterium
CROWN GALL
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
topic Agronomía, reproducción y protección de plantas
Agrobacterium
CROWN GALL
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
dc.description.none.fl_txt_mv The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina.
description The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/7195
url https://digital.cic.gba.gob.ar/handle/11746/7195
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
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collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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