Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina

Autores
Alippi, Adriana Mónica; López, Ana Claudia; Balatti, Pedro Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
Centro de Investigaciones en Fitopatología
Materia
Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131705
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in ArgentinaAlippi, Adriana MónicaLópez, Ana ClaudiaBalatti, Pedro AlbertoBotánicaAgrobacterium rubiAgrobacterium tumefaciensAgrobacterium rhizogenesBiovarsVaccinium corymbossumBlueberriesCrown gall diseaseDiversityPCRThe aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.Centro de Investigaciones en Fitopatología2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf415-430http://sedici.unlp.edu.ar/handle/10915/131705enginfo:eu-repo/semantics/altIdentifier/issn/0929-1873info:eu-repo/semantics/altIdentifier/issn/1573-8469info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-012-0001-Xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:39Zoai:sedici.unlp.edu.ar:10915/131705Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:39.245SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
spellingShingle Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
Alippi, Adriana Mónica
Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
title_short Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_full Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_fullStr Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_full_unstemmed Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_sort Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
López, Ana Claudia
Balatti, Pedro Alberto
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
López, Ana Claudia
Balatti, Pedro Alberto
author_role author
author2 López, Ana Claudia
Balatti, Pedro Alberto
author2_role author
author
dc.subject.none.fl_str_mv Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
topic Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
dc.description.none.fl_txt_mv The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
Centro de Investigaciones en Fitopatología
description The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131705
url http://sedici.unlp.edu.ar/handle/10915/131705
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0929-1873
info:eu-repo/semantics/altIdentifier/issn/1573-8469
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-012-0001-X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
415-430
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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