Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus

Autores
Acosta, E.G.; Castilla, V.; Damonte, E.B.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1) strain HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of the slow recycling pathway for DENV-2 productive infection. © 2012 Acosta et al.
Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
PLoS ONE 2012;7(9)
Materia
ammonium chloride
chlorpromazine
clathrin
Rab protein
Rab7 protein
virus RNA
animal cell
article
cell migration
cellular distribution
controlled study
cytoplasm
dengue
Dengue virus 1
Dengue virus 2
drug efficacy
endocytosis
endosome
enzyme inhibition
enzyme regulation
nonhuman
protein expression
treatment response
Vero cell
virus entry
virus nucleocapsid
Animals
Cercopithecus aethiops
Dengue Virus
Endocytosis
Fluorescent Antibody Technique
Membrane Fusion
rab GTP-Binding Proteins
Vero Cells
Virus Replication
Dengue virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_19326203_v7_n9_p_Acosta
Acceder
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oai_identifier_str paperaa:paper_19326203_v7_n9_p_Acosta
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Differential Requirements in Endocytic Trafficking for Penetration of Dengue VirusAcosta, E.G.Castilla, V.Damonte, E.B.ammonium chloridechlorpromazineclathrinRab proteinRab7 proteinvirus RNAanimal cellarticlecell migrationcellular distributioncontrolled studycytoplasmdengueDengue virus 1Dengue virus 2drug efficacyendocytosisendosomeenzyme inhibitionenzyme regulationnonhumanprotein expressiontreatment responseVero cellvirus entryvirus nucleocapsidAnimalsCercopithecus aethiopsDengue VirusEndocytosisFluorescent Antibody TechniqueMembrane Fusionrab GTP-Binding ProteinsVero CellsVirus ReplicationDengue virusThe entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1) strain HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of the slow recycling pathway for DENV-2 productive infection. © 2012 Acosta et al.Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_19326203_v7_n9_p_AcostaPLoS ONE 2012;7(9)reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_19326203_v7_n9_p_AcostaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.038Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
title Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
spellingShingle Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
Acosta, E.G.
ammonium chloride
chlorpromazine
clathrin
Rab protein
Rab7 protein
virus RNA
animal cell
article
cell migration
cellular distribution
controlled study
cytoplasm
dengue
Dengue virus 1
Dengue virus 2
drug efficacy
endocytosis
endosome
enzyme inhibition
enzyme regulation
nonhuman
protein expression
treatment response
Vero cell
virus entry
virus nucleocapsid
Animals
Cercopithecus aethiops
Dengue Virus
Endocytosis
Fluorescent Antibody Technique
Membrane Fusion
rab GTP-Binding Proteins
Vero Cells
Virus Replication
Dengue virus
title_short Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
title_full Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
title_fullStr Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
title_full_unstemmed Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
title_sort Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus
dc.creator.none.fl_str_mv Acosta, E.G.
Castilla, V.
Damonte, E.B.
author Acosta, E.G.
author_facet Acosta, E.G.
Castilla, V.
Damonte, E.B.
author_role author
author2 Castilla, V.
Damonte, E.B.
author2_role author
author
dc.subject.none.fl_str_mv ammonium chloride
chlorpromazine
clathrin
Rab protein
Rab7 protein
virus RNA
animal cell
article
cell migration
cellular distribution
controlled study
cytoplasm
dengue
Dengue virus 1
Dengue virus 2
drug efficacy
endocytosis
endosome
enzyme inhibition
enzyme regulation
nonhuman
protein expression
treatment response
Vero cell
virus entry
virus nucleocapsid
Animals
Cercopithecus aethiops
Dengue Virus
Endocytosis
Fluorescent Antibody Technique
Membrane Fusion
rab GTP-Binding Proteins
Vero Cells
Virus Replication
Dengue virus
topic ammonium chloride
chlorpromazine
clathrin
Rab protein
Rab7 protein
virus RNA
animal cell
article
cell migration
cellular distribution
controlled study
cytoplasm
dengue
Dengue virus 1
Dengue virus 2
drug efficacy
endocytosis
endosome
enzyme inhibition
enzyme regulation
nonhuman
protein expression
treatment response
Vero cell
virus entry
virus nucleocapsid
Animals
Cercopithecus aethiops
Dengue Virus
Endocytosis
Fluorescent Antibody Technique
Membrane Fusion
rab GTP-Binding Proteins
Vero Cells
Virus Replication
Dengue virus
dc.description.none.fl_txt_mv The entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1) strain HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of the slow recycling pathway for DENV-2 productive infection. © 2012 Acosta et al.
Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1) strain HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of the slow recycling pathway for DENV-2 productive infection. © 2012 Acosta et al.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_19326203_v7_n9_p_Acosta
url http://hdl.handle.net/20.500.12110/paper_19326203_v7_n9_p_Acosta
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 2012;7(9)
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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