Interference in dengue virus adsorption and uncoating by carrageenans
- Autores
- Talarico, L.B.; Damonte, E.B.
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.
Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Virology 2007;363(2):473-485
- Materia
-
Adsorption
Carrageenan
Dengue virus
Flavivirus
Heparan sulfate
Internalization
Uncoating
Virus entry
antivirus agent
carrageenan
coat protein
heparan sulfate
animal cell
antigen expression
antiviral activity
article
controlled study
dengue
Dengue virus
dose response
dose time effect relation
drug inhibition
drug structure
endosome
Flavivirus
host cell
human
human cell
internalization
macromolecule
nonhuman
priority journal
protein synthesis
reverse transcription polymerase chain reaction
virion
virogenesis
virus adsorption
virus inhibition
virus nucleocapsid
Animals
Carrageenan
Cell Line
Dengue
Dengue Virus
Dose-Response Relationship, Drug
Humans
Virus Replication
Dengue virus
Dengue virus type 2
Flavivirus - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00426822_v363_n2_p473_Talarico
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Interference in dengue virus adsorption and uncoating by carrageenansTalarico, L.B.Damonte, E.B.AdsorptionCarrageenanDengue virusFlavivirusHeparan sulfateInternalizationUncoatingVirus entryantivirus agentcarrageenancoat proteinheparan sulfateanimal cellantigen expressionantiviral activityarticlecontrolled studydengueDengue virusdose responsedose time effect relationdrug inhibitiondrug structureendosomeFlavivirushost cellhumanhuman cellinternalizationmacromoleculenonhumanpriority journalprotein synthesisreverse transcription polymerase chain reactionvirionvirogenesisvirus adsorptionvirus inhibitionvirus nucleocapsidAnimalsCarrageenanCell LineDengueDengue VirusDose-Response Relationship, DrugHumansVirus ReplicationDengue virusDengue virus type 2FlavivirusThis study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_TalaricoVirology 2007;363(2):473-485reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:13Zpaperaa:paper_00426822_v363_n2_p473_TalaricoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:14.886Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Interference in dengue virus adsorption and uncoating by carrageenans |
title |
Interference in dengue virus adsorption and uncoating by carrageenans |
spellingShingle |
Interference in dengue virus adsorption and uncoating by carrageenans Talarico, L.B. Adsorption Carrageenan Dengue virus Flavivirus Heparan sulfate Internalization Uncoating Virus entry antivirus agent carrageenan coat protein heparan sulfate animal cell antigen expression antiviral activity article controlled study dengue Dengue virus dose response dose time effect relation drug inhibition drug structure endosome Flavivirus host cell human human cell internalization macromolecule nonhuman priority journal protein synthesis reverse transcription polymerase chain reaction virion virogenesis virus adsorption virus inhibition virus nucleocapsid Animals Carrageenan Cell Line Dengue Dengue Virus Dose-Response Relationship, Drug Humans Virus Replication Dengue virus Dengue virus type 2 Flavivirus |
title_short |
Interference in dengue virus adsorption and uncoating by carrageenans |
title_full |
Interference in dengue virus adsorption and uncoating by carrageenans |
title_fullStr |
Interference in dengue virus adsorption and uncoating by carrageenans |
title_full_unstemmed |
Interference in dengue virus adsorption and uncoating by carrageenans |
title_sort |
Interference in dengue virus adsorption and uncoating by carrageenans |
dc.creator.none.fl_str_mv |
Talarico, L.B. Damonte, E.B. |
author |
Talarico, L.B. |
author_facet |
Talarico, L.B. Damonte, E.B. |
author_role |
author |
author2 |
Damonte, E.B. |
author2_role |
author |
dc.subject.none.fl_str_mv |
Adsorption Carrageenan Dengue virus Flavivirus Heparan sulfate Internalization Uncoating Virus entry antivirus agent carrageenan coat protein heparan sulfate animal cell antigen expression antiviral activity article controlled study dengue Dengue virus dose response dose time effect relation drug inhibition drug structure endosome Flavivirus host cell human human cell internalization macromolecule nonhuman priority journal protein synthesis reverse transcription polymerase chain reaction virion virogenesis virus adsorption virus inhibition virus nucleocapsid Animals Carrageenan Cell Line Dengue Dengue Virus Dose-Response Relationship, Drug Humans Virus Replication Dengue virus Dengue virus type 2 Flavivirus |
topic |
Adsorption Carrageenan Dengue virus Flavivirus Heparan sulfate Internalization Uncoating Virus entry antivirus agent carrageenan coat protein heparan sulfate animal cell antigen expression antiviral activity article controlled study dengue Dengue virus dose response dose time effect relation drug inhibition drug structure endosome Flavivirus host cell human human cell internalization macromolecule nonhuman priority journal protein synthesis reverse transcription polymerase chain reaction virion virogenesis virus adsorption virus inhibition virus nucleocapsid Animals Carrageenan Cell Line Dengue Dengue Virus Dose-Response Relationship, Drug Humans Virus Replication Dengue virus Dengue virus type 2 Flavivirus |
dc.description.none.fl_txt_mv |
This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved. Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_Talarico |
url |
http://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_Talarico |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Virology 2007;363(2):473-485 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
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Biblioteca Digital (UBA-FCEN) |
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Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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