Interference in dengue virus adsorption and uncoating by carrageenans

Autores
Talarico, L.B.; Damonte, E.B.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.
Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Virology 2007;363(2):473-485
Materia
Adsorption
Carrageenan
Dengue virus
Flavivirus
Heparan sulfate
Internalization
Uncoating
Virus entry
antivirus agent
carrageenan
coat protein
heparan sulfate
animal cell
antigen expression
antiviral activity
article
controlled study
dengue
Dengue virus
dose response
dose time effect relation
drug inhibition
drug structure
endosome
Flavivirus
host cell
human
human cell
internalization
macromolecule
nonhuman
priority journal
protein synthesis
reverse transcription polymerase chain reaction
virion
virogenesis
virus adsorption
virus inhibition
virus nucleocapsid
Animals
Carrageenan
Cell Line
Dengue
Dengue Virus
Dose-Response Relationship, Drug
Humans
Virus Replication
Dengue virus
Dengue virus type 2
Flavivirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00426822_v363_n2_p473_Talarico

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oai_identifier_str paperaa:paper_00426822_v363_n2_p473_Talarico
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Interference in dengue virus adsorption and uncoating by carrageenansTalarico, L.B.Damonte, E.B.AdsorptionCarrageenanDengue virusFlavivirusHeparan sulfateInternalizationUncoatingVirus entryantivirus agentcarrageenancoat proteinheparan sulfateanimal cellantigen expressionantiviral activityarticlecontrolled studydengueDengue virusdose responsedose time effect relationdrug inhibitiondrug structureendosomeFlavivirushost cellhumanhuman cellinternalizationmacromoleculenonhumanpriority journalprotein synthesisreverse transcription polymerase chain reactionvirionvirogenesisvirus adsorptionvirus inhibitionvirus nucleocapsidAnimalsCarrageenanCell LineDengueDengue VirusDose-Response Relationship, DrugHumansVirus ReplicationDengue virusDengue virus type 2FlavivirusThis study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_TalaricoVirology 2007;363(2):473-485reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:13Zpaperaa:paper_00426822_v363_n2_p473_TalaricoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:14.886Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Interference in dengue virus adsorption and uncoating by carrageenans
title Interference in dengue virus adsorption and uncoating by carrageenans
spellingShingle Interference in dengue virus adsorption and uncoating by carrageenans
Talarico, L.B.
Adsorption
Carrageenan
Dengue virus
Flavivirus
Heparan sulfate
Internalization
Uncoating
Virus entry
antivirus agent
carrageenan
coat protein
heparan sulfate
animal cell
antigen expression
antiviral activity
article
controlled study
dengue
Dengue virus
dose response
dose time effect relation
drug inhibition
drug structure
endosome
Flavivirus
host cell
human
human cell
internalization
macromolecule
nonhuman
priority journal
protein synthesis
reverse transcription polymerase chain reaction
virion
virogenesis
virus adsorption
virus inhibition
virus nucleocapsid
Animals
Carrageenan
Cell Line
Dengue
Dengue Virus
Dose-Response Relationship, Drug
Humans
Virus Replication
Dengue virus
Dengue virus type 2
Flavivirus
title_short Interference in dengue virus adsorption and uncoating by carrageenans
title_full Interference in dengue virus adsorption and uncoating by carrageenans
title_fullStr Interference in dengue virus adsorption and uncoating by carrageenans
title_full_unstemmed Interference in dengue virus adsorption and uncoating by carrageenans
title_sort Interference in dengue virus adsorption and uncoating by carrageenans
dc.creator.none.fl_str_mv Talarico, L.B.
Damonte, E.B.
author Talarico, L.B.
author_facet Talarico, L.B.
Damonte, E.B.
author_role author
author2 Damonte, E.B.
author2_role author
dc.subject.none.fl_str_mv Adsorption
Carrageenan
Dengue virus
Flavivirus
Heparan sulfate
Internalization
Uncoating
Virus entry
antivirus agent
carrageenan
coat protein
heparan sulfate
animal cell
antigen expression
antiviral activity
article
controlled study
dengue
Dengue virus
dose response
dose time effect relation
drug inhibition
drug structure
endosome
Flavivirus
host cell
human
human cell
internalization
macromolecule
nonhuman
priority journal
protein synthesis
reverse transcription polymerase chain reaction
virion
virogenesis
virus adsorption
virus inhibition
virus nucleocapsid
Animals
Carrageenan
Cell Line
Dengue
Dengue Virus
Dose-Response Relationship, Drug
Humans
Virus Replication
Dengue virus
Dengue virus type 2
Flavivirus
topic Adsorption
Carrageenan
Dengue virus
Flavivirus
Heparan sulfate
Internalization
Uncoating
Virus entry
antivirus agent
carrageenan
coat protein
heparan sulfate
animal cell
antigen expression
antiviral activity
article
controlled study
dengue
Dengue virus
dose response
dose time effect relation
drug inhibition
drug structure
endosome
Flavivirus
host cell
human
human cell
internalization
macromolecule
nonhuman
priority journal
protein synthesis
reverse transcription polymerase chain reaction
virion
virogenesis
virus adsorption
virus inhibition
virus nucleocapsid
Animals
Carrageenan
Cell Line
Dengue
Dengue Virus
Dose-Response Relationship, Drug
Humans
Virus Replication
Dengue virus
Dengue virus type 2
Flavivirus
dc.description.none.fl_txt_mv This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.
Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description This study demonstrated that the λ- and ι-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (DENV-2) and 3 (DENV-3) multiplication in Vero and HepG2 cells, with values of effective concentration 50% from 0.14 to 4.1 μg/ml. This activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.001-1. The inhibitory action of the λ-carrageenan, an heparan sulfate (HS)-imitative compound, was exerted by a dual interference with virus adsorption and internalization of nucleocapsid into the cytoplasm. Although virus particles may enter the cell when compound was added after DENV-2 adsorption, as shown by intracellular uptake of radiolabeled DENV-2 particles and quantitative RT-PCR, infectious center and virion uncoating assays have shown that carrageenan-treated virions cannot be released from the endosomes. Viral protein synthesis, the first step of macromolecular synthesis after DENV entry to the host cell, was not affected by the carrageenan. Furthermore, no inhibition of virus multiplication was detected when the entry process was bypassed through DENV-2 RNA transfection into the cell. The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy. © 2007 Elsevier Inc. All rights reserved.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_Talarico
url http://hdl.handle.net/20.500.12110/paper_00426822_v363_n2_p473_Talarico
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virology 2007;363(2):473-485
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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