Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth

Autores
Prado-Acosta, M.; Ruzal, S.M.; Allievi, M.C.; Palomino, M.M.; Rivas, C.S.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 2010;76(3):974-977
Materia
Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00992240_v76_n3_p974_PradoAcosta
Acceder
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oai_identifier_str paperaa:paper_00992240_v76_n3_p974_PradoAcosta
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growthPrado-Acosta, M.Ruzal, S.M.Allievi, M.C.Palomino, M.M.Rivas, C.S.BacilliBacillus cereusBacteriologyCytologyHydrolasesSalmonellaBacterial growthGram-positive bacteriumHydrolase activitiesLactobacillus acidophilusProton-motive forcesSalmonella entericaStaphylococcus aureusSynergistic effectAntibioticsbacteriumenzyme activitygrowth ratemembranesurface layersynergismBacillus cereusBacteria (microorganisms)Lactobacillus acidophilusNegibacteriaPosibacteriaSalmonella entericaStaphylococcus aureusWe have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcostaAppl. Environ. Microbiol. 2010;76(3):974-977reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-11-06T09:39:39Zpaperaa:paper_00992240_v76_n3_p974_PradoAcostaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-11-06 09:39:41.568Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
spellingShingle Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
Prado-Acosta, M.
Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
title_short Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_fullStr Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full_unstemmed Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_sort Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
dc.creator.none.fl_str_mv Prado-Acosta, M.
Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
author Prado-Acosta, M.
author_facet Prado-Acosta, M.
Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
author_role author
author2 Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
topic Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
dc.description.none.fl_txt_mv We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
url http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Appl. Environ. Microbiol. 2010;76(3):974-977
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.976206

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