Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
- Autores
- Lauricella, M.A.; Castañera, M.B.; Gürtler, R.E.; Segura, E.L.
- Año de publicación
- 1998
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.
Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Mem. Inst. Oswaldo Cruz 1998;93(4):501-507
- Materia
-
Chronic infection
Dogs
Serology
Surveillance
Trypanosoma cruzi
Canis familiaris
Trypanosoma
Trypanosoma cruzi
protozoon antibody
animal
animal disease
Argentina
article
blood
Chagas disease
dog
dog disease
enzyme linked immunosorbent assay
hemagglutination test
immunology
sensitivity and specificity
standard
Trypanosoma cruzi
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Dog Diseases
Dogs
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Sensitivity and Specificity
Trypanosoma cruzi - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00740276_v93_n4_p501_Lauricella
Ver los metadatos del registro completo
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paperaa:paper_00740276_v93_n4_p501_Lauricella |
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network_name_str |
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spelling |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected DogsLauricella, M.A.Castañera, M.B.Gürtler, R.E.Segura, E.L.Chronic infectionDogsSerologySurveillanceTrypanosoma cruziCanis familiarisTrypanosomaTrypanosoma cruziprotozoon antibodyanimalanimal diseaseArgentinaarticlebloodChagas diseasedogdog diseaseenzyme linked immunosorbent assayhemagglutination testimmunologysensitivity and specificitystandardTrypanosoma cruziAnimalsAntibodies, ProtozoanArgentinaChagas DiseaseDog DiseasesDogsEnzyme-Linked Immunosorbent AssayHemagglutination TestsSensitivity and SpecificityTrypanosoma cruziThis study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_LauricellaMem. Inst. Oswaldo Cruz 1998;93(4):501-507reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:57Zpaperaa:paper_00740276_v93_n4_p501_LauricellaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.069Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
title |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
spellingShingle |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs Lauricella, M.A. Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi |
title_short |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
title_full |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
title_fullStr |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
title_full_unstemmed |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
title_sort |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
dc.creator.none.fl_str_mv |
Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. |
author |
Lauricella, M.A. |
author_facet |
Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. |
author_role |
author |
author2 |
Castañera, M.B. Gürtler, R.E. Segura, E.L. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi |
topic |
Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi |
dc.description.none.fl_txt_mv |
This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations. Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella |
url |
http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Mem. Inst. Oswaldo Cruz 1998;93(4):501-507 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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1844618736313565184 |
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13.070432 |