Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs

Autores
Lauricella, M.A.; Castañera, M.B.; Gürtler, R.E.; Segura, E.L.
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.
Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Mem. Inst. Oswaldo Cruz 1998;93(4):501-507
Materia
Chronic infection
Dogs
Serology
Surveillance
Trypanosoma cruzi
Canis familiaris
Trypanosoma
Trypanosoma cruzi
protozoon antibody
animal
animal disease
Argentina
article
blood
Chagas disease
dog
dog disease
enzyme linked immunosorbent assay
hemagglutination test
immunology
sensitivity and specificity
standard
Trypanosoma cruzi
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Dog Diseases
Dogs
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Sensitivity and Specificity
Trypanosoma cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00740276_v93_n4_p501_Lauricella

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oai_identifier_str paperaa:paper_00740276_v93_n4_p501_Lauricella
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected DogsLauricella, M.A.Castañera, M.B.Gürtler, R.E.Segura, E.L.Chronic infectionDogsSerologySurveillanceTrypanosoma cruziCanis familiarisTrypanosomaTrypanosoma cruziprotozoon antibodyanimalanimal diseaseArgentinaarticlebloodChagas diseasedogdog diseaseenzyme linked immunosorbent assayhemagglutination testimmunologysensitivity and specificitystandardTrypanosoma cruziAnimalsAntibodies, ProtozoanArgentinaChagas DiseaseDog DiseasesDogsEnzyme-Linked Immunosorbent AssayHemagglutination TestsSensitivity and SpecificityTrypanosoma cruziThis study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_LauricellaMem. Inst. Oswaldo Cruz 1998;93(4):501-507reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:57Zpaperaa:paper_00740276_v93_n4_p501_LauricellaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.069Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
title Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
spellingShingle Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
Lauricella, M.A.
Chronic infection
Dogs
Serology
Surveillance
Trypanosoma cruzi
Canis familiaris
Trypanosoma
Trypanosoma cruzi
protozoon antibody
animal
animal disease
Argentina
article
blood
Chagas disease
dog
dog disease
enzyme linked immunosorbent assay
hemagglutination test
immunology
sensitivity and specificity
standard
Trypanosoma cruzi
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Dog Diseases
Dogs
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Sensitivity and Specificity
Trypanosoma cruzi
title_short Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
title_full Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
title_fullStr Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
title_full_unstemmed Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
title_sort Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
dc.creator.none.fl_str_mv Lauricella, M.A.
Castañera, M.B.
Gürtler, R.E.
Segura, E.L.
author Lauricella, M.A.
author_facet Lauricella, M.A.
Castañera, M.B.
Gürtler, R.E.
Segura, E.L.
author_role author
author2 Castañera, M.B.
Gürtler, R.E.
Segura, E.L.
author2_role author
author
author
dc.subject.none.fl_str_mv Chronic infection
Dogs
Serology
Surveillance
Trypanosoma cruzi
Canis familiaris
Trypanosoma
Trypanosoma cruzi
protozoon antibody
animal
animal disease
Argentina
article
blood
Chagas disease
dog
dog disease
enzyme linked immunosorbent assay
hemagglutination test
immunology
sensitivity and specificity
standard
Trypanosoma cruzi
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Dog Diseases
Dogs
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Sensitivity and Specificity
Trypanosoma cruzi
topic Chronic infection
Dogs
Serology
Surveillance
Trypanosoma cruzi
Canis familiaris
Trypanosoma
Trypanosoma cruzi
protozoon antibody
animal
animal disease
Argentina
article
blood
Chagas disease
dog
dog disease
enzyme linked immunosorbent assay
hemagglutination test
immunology
sensitivity and specificity
standard
Trypanosoma cruzi
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Dog Diseases
Dogs
Enzyme-Linked Immunosorbent Assay
Hemagglutination Tests
Sensitivity and Specificity
Trypanosoma cruzi
dc.description.none.fl_txt_mv This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.
Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella
url http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Mem. Inst. Oswaldo Cruz 1998;93(4):501-507
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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