The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Autores
Santos, M.R.M.; Cano, M.I.; Schijman, A.; Lorenzi, H.; Vázquez, M.; Levin, M.J.; Ramirez, J.L.; Brandão, A.; Degrave, W.M.; Da Silveira, J.F.
Año de publicación
1997
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
Fil:Schijman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Lorenzi, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vázquez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Mem. Inst. Oswaldo Cruz 1997;92(6):821-828
Materia
Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00740276_v92_n6_p821_Santos
Acceder
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oai_identifier_str paperaa:paper_00740276_v92_n6_p821_Santos
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL BrenerSantos, M.R.M.Cano, M.I.Schijman, A.Lorenzi, H.Vázquez, M.Levin, M.J.Ramirez, J.L.Brandão, A.Degrave, W.M.Da Silveira, J.F.Chromosome mappingClone CL BrenerGenetic mapGenome projectMolecular karyotypeTrypanosoma cruziTrypanosomaTrypanosoma cruzianimalchromosome mapcloninggeneticsgenomekaryotypingreviewTrypanosoma cruziAnimalsChromosome MappingCloning, OrganismGenome, ProtozoanKaryotypingTrypanosoma cruziBy using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.Fil:Schijman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Lorenzi, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Vázquez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_SantosMem. Inst. Oswaldo Cruz 1997;92(6):821-828reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-11T10:21:44Zpaperaa:paper_00740276_v92_n6_p821_SantosInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-11 10:21:45.438Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
spellingShingle The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
Santos, M.R.M.
Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
title_short The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_fullStr The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full_unstemmed The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_sort The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
dc.creator.none.fl_str_mv Santos, M.R.M.
Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
author Santos, M.R.M.
author_facet Santos, M.R.M.
Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
author_role author
author2 Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
topic Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
dc.description.none.fl_txt_mv By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
Fil:Schijman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Lorenzi, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vázquez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
publishDate 1997
dc.date.none.fl_str_mv 1997
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_Santos
url http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_Santos
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Mem. Inst. Oswaldo Cruz 1997;92(6):821-828
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.993085

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