Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants

Autores
Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The response of tobacco (Nicotiana tabacum L.) wild type SR1 and catalase-deficient CAT1AS plants was evaluated after exposure to CdCl2. CAT1AS plants accumulated more Cd than SR1 plants, and this was associated with reduced growth, but higher chlorophyll content and cell viability. Despite catalase deficiency, CAT1AS plants did not accumulate more H2O2 than the wild line when exposed to Cd, probably due to the fact that CAT1AS plants counterbalanced their catalase deficiency by increasing the constitutive guaiacol peroxidase and ascorbate peroxidase activities and by reducing the basal NADPH oxidase-like enzyme activity. Both lines could activate their antioxidant system upon Cd stress, although the stress response pathways showed wide differences in the mineral and nitrogen metabolism, since the wild-type line had reduced nitrates and iron content, while CAT1AS maintained the same level of nitrates and Fe than that of non-treated plants, and responded with a significant increase in proline. The results showed that, unlike previous reports using other type of stress with the same line plants, catalase did not play a crucial role in protecting against Cd toxicity and CAT1AS plants, compared to SR1, were able to activate alternative defence mechanisms against Cd toxicity.
Fil: Iannone, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Groppa, María Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cadmium
Hydrogen Peroxide
Nicotiana Tabacum
Catalase
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17935
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17935
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plantsIannone, María FlorenciaGroppa, María DanielaBenavides, Maria PatriciaCadmiumHydrogen PeroxideNicotiana TabacumCatalaseReactive Oxygen Specieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The response of tobacco (Nicotiana tabacum L.) wild type SR1 and catalase-deficient CAT1AS plants was evaluated after exposure to CdCl2. CAT1AS plants accumulated more Cd than SR1 plants, and this was associated with reduced growth, but higher chlorophyll content and cell viability. Despite catalase deficiency, CAT1AS plants did not accumulate more H2O2 than the wild line when exposed to Cd, probably due to the fact that CAT1AS plants counterbalanced their catalase deficiency by increasing the constitutive guaiacol peroxidase and ascorbate peroxidase activities and by reducing the basal NADPH oxidase-like enzyme activity. Both lines could activate their antioxidant system upon Cd stress, although the stress response pathways showed wide differences in the mineral and nitrogen metabolism, since the wild-type line had reduced nitrates and iron content, while CAT1AS maintained the same level of nitrates and Fe than that of non-treated plants, and responded with a significant increase in proline. The results showed that, unlike previous reports using other type of stress with the same line plants, catalase did not play a crucial role in protecting against Cd toxicity and CAT1AS plants, compared to SR1, were able to activate alternative defence mechanisms against Cd toxicity.Fil: Iannone, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Groppa, María Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17935Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia; Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants; Elsevier Science; Environmental and Experimental Botany; 109; 1-2015; 201-2110098-8472enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0098847214001798info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2014.07.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:06Zoai:ri.conicet.gov.ar:11336/17935instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:07.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
title Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
spellingShingle Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
Iannone, María Florencia
Cadmium
Hydrogen Peroxide
Nicotiana Tabacum
Catalase
Reactive Oxygen Species
title_short Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
title_full Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
title_fullStr Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
title_full_unstemmed Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
title_sort Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants
dc.creator.none.fl_str_mv Iannone, María Florencia
Groppa, María Daniela
Benavides, Maria Patricia
author Iannone, María Florencia
author_facet Iannone, María Florencia
Groppa, María Daniela
Benavides, Maria Patricia
author_role author
author2 Groppa, María Daniela
Benavides, Maria Patricia
author2_role author
author
dc.subject.none.fl_str_mv Cadmium
Hydrogen Peroxide
Nicotiana Tabacum
Catalase
Reactive Oxygen Species
topic Cadmium
Hydrogen Peroxide
Nicotiana Tabacum
Catalase
Reactive Oxygen Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The response of tobacco (Nicotiana tabacum L.) wild type SR1 and catalase-deficient CAT1AS plants was evaluated after exposure to CdCl2. CAT1AS plants accumulated more Cd than SR1 plants, and this was associated with reduced growth, but higher chlorophyll content and cell viability. Despite catalase deficiency, CAT1AS plants did not accumulate more H2O2 than the wild line when exposed to Cd, probably due to the fact that CAT1AS plants counterbalanced their catalase deficiency by increasing the constitutive guaiacol peroxidase and ascorbate peroxidase activities and by reducing the basal NADPH oxidase-like enzyme activity. Both lines could activate their antioxidant system upon Cd stress, although the stress response pathways showed wide differences in the mineral and nitrogen metabolism, since the wild-type line had reduced nitrates and iron content, while CAT1AS maintained the same level of nitrates and Fe than that of non-treated plants, and responded with a significant increase in proline. The results showed that, unlike previous reports using other type of stress with the same line plants, catalase did not play a crucial role in protecting against Cd toxicity and CAT1AS plants, compared to SR1, were able to activate alternative defence mechanisms against Cd toxicity.
Fil: Iannone, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Groppa, María Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The response of tobacco (Nicotiana tabacum L.) wild type SR1 and catalase-deficient CAT1AS plants was evaluated after exposure to CdCl2. CAT1AS plants accumulated more Cd than SR1 plants, and this was associated with reduced growth, but higher chlorophyll content and cell viability. Despite catalase deficiency, CAT1AS plants did not accumulate more H2O2 than the wild line when exposed to Cd, probably due to the fact that CAT1AS plants counterbalanced their catalase deficiency by increasing the constitutive guaiacol peroxidase and ascorbate peroxidase activities and by reducing the basal NADPH oxidase-like enzyme activity. Both lines could activate their antioxidant system upon Cd stress, although the stress response pathways showed wide differences in the mineral and nitrogen metabolism, since the wild-type line had reduced nitrates and iron content, while CAT1AS maintained the same level of nitrates and Fe than that of non-treated plants, and responded with a significant increase in proline. The results showed that, unlike previous reports using other type of stress with the same line plants, catalase did not play a crucial role in protecting against Cd toxicity and CAT1AS plants, compared to SR1, were able to activate alternative defence mechanisms against Cd toxicity.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17935
Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia; Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants; Elsevier Science; Environmental and Experimental Botany; 109; 1-2015; 201-211
0098-8472
url http://hdl.handle.net/11336/17935
identifier_str_mv Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia; Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants; Elsevier Science; Environmental and Experimental Botany; 109; 1-2015; 201-211
0098-8472
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0098847214001798
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2014.07.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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