Hypothyroid phenotype is contributed by mitochondrial complex I inactivation due to translocated neuronal nitric-oxide synthase

• Autores: Franco, M.C.; Antico Arciuch, V.G.; Peralta, J.G.; Galli, S.; Levisman, D.; López, L.M.; Romorini, L.; Poderoso, J.J.; Carreras, M.C.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. Biol. Chem. 2006;281(8):4779-4786
• Materia: Complexation; Hormones; Metabolism; Neurology; Oxides; Proteins; RNA; Hypothyroid; Mitochondria; Mitochondrial complex I; Neuronal nitric-oxide synthase; Nitric acid; 3,3',5' triiodothyronine; cyclin D1; liver enzyme; messenger RNA; mitochondrial complex 1; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; n(g) nitroarginine methyl ester; neuronal nitric oxide synthase; neuronal nitric oxide synthase alpha; nitric oxide; oxidoreductase; peroxynitrite; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); thiamazole; thyrotropin; tyrosine; unclassified drug; cyclin D1; heat shock protein 90; isoprotein; messenger RNA; mitogen activated protein kinase p38; n(g) nitroarginine methyl ester; neuronal nitric oxide synthase; nitric oxide synthase; oxidizing agent; oxygen; peroxynitrous acid; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); thyroid hormone; animal cell; animal experiment; animal tissue; article; basal metabolic rate; cell communication; cellular distribution; controlled study; enzyme activity; enzyme inactivation; enzyme localization; hypothyroidism; liothyronine blood level; male; mitochondrial respiration; nitration; nonhuman; oxygen consumption; phenotype; priority journal; protein expression; protein transport; rat; signal transduction; animal; cell fractionation; chemical model; chemistry; cytosol; electron; enzymology; genetic transcription; hypothyroidism; immunoblotting; immunoelectron microscopy; immunoprecipitation; liver; liver mitochondrion; metabolism; mitochondrion; pathology; polyacrylamide gel electrophoresis; reverse transcription polymerase chain reaction; Wistar rat; Animals; Cyclin D1; Cytosol; Electron Transport Complex I; Electrons; Electrophoresis, Polyacrylamide Gel; HSP90 Heat-Shock Proteins; Hypothyroidism; Immunoblotting; Immunoprecipitation; Liver; Male; MAP Kinase Signaling System; Microscopy, Immunoelectron; Mitochondria; Mitochondria, Liver; Models, Chemical; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase; Nitric Oxide Synthase Type I; Oxidants; Oxygen; p38 Mitogen-Activated Protein Kinases; Peroxynitrous Acid; Phenotype; Protein Isoforms; Protein Transport; Rats; Rats, Wistar; Reactive Oxygen Species; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Signal Transduction; Subcellular Fractions; Thyroid Hormones; Transcription, Genetic
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Although transcriptional effects of thyroid hormones have substantial influence on oxidative metabolism, how thyroid sets basal metabolic rate remains obscure. Compartmental localization of nitric-oxide synthases is important for nitric oxide signaling. We therefore examined liver neuronal nitric-oxide synthase-α (nNOS) subcellular distribution as a putative mechanism for thyroid effects on rat metabolic rate. At low 3,3′,5-triiodo-L-thyronine levels, nNOS mRNA increased by 3-fold, protein expression by one-fold, and nNOS was selectively translocated to mitochondria without changes in other isoforms. In contrast, under thyroid hormone administration, mRNA level did not change and nNOS remained predominantly localized in cytosol. In hypothyroidism, nNOS translocation resulted in enhanced mitochondrial nitric-oxide synthase activity with low O2 uptake. In this context, NO utilization increased active O2 species and peroxynitrite yields and tyrosine nitration of complex I proteins that reduced complex activity. Hypothyroidism was also associated to high phospho-p38 mitogen-activated protein kinase and decreased phospho-extracellular signal-regulated kinase 1/2 and cyclin D1 levels. Similarly to thyroid hormones, but without changing thyroid status, nitric-oxide synthase inhibitor Nω-nitro-L-arginine methyl ester increased basal metabolic rate, prevented mitochondrial nitration and complex I derangement, and turned mitogen-activated protein kinase signaling and cyclin D1 expression back to control pattern. We surmise that nNOS spatial confinement in mitochondria is a significant downstream effector of thyroid hormone and hypothyroid phenotype. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Fil:Franco, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Antico Arciuch, V.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Galli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.