Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti

Autores
Ugalde, R.A.; Coira, J.A.; Brill, W.J.
Año de publicación
1986
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.
Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. BACTERIOL. 1986;168(1):270-275
Materia
galactose
galacturonic acid
polysaccharide
uridine diphosphate galactose
uridine diphosphate glucuronic acid
article
nonhuman
priority journal
Sinorhizobium meliloti
Medicago sativa
Rhizobium
Scophthalmus rhombus
Sinorhizobium meliloti
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00219193_v168_n1_p270_Ugalde
Acceder
id BDUBAFCEN_71f6f7de3a6a9f9716bb1fc42da0c5a5
oai_identifier_str paperaa:paper_00219193_v168_n1_p270_Ugalde
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium melilotiUgalde, R.A.Coira, J.A.Brill, W.J.galactosegalacturonic acidpolysaccharideuridine diphosphate galactoseuridine diphosphate glucuronic acidarticlenonhumanpriority journalSinorhizobium melilotiMedicago sativaRhizobiumScophthalmus rhombusSinorhizobium melilotiPrevious work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1986info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_UgaldeJ. BACTERIOL. 1986;168(1):270-275reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-23T11:18:15Zpaperaa:paper_00219193_v168_n1_p270_UgaldeInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-23 11:18:16.934Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
title Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
spellingShingle Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
Ugalde, R.A.
galactose
galacturonic acid
polysaccharide
uridine diphosphate galactose
uridine diphosphate glucuronic acid
article
nonhuman
priority journal
Sinorhizobium meliloti
Medicago sativa
Rhizobium
Scophthalmus rhombus
Sinorhizobium meliloti
title_short Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
title_full Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
title_fullStr Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
title_full_unstemmed Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
title_sort Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
dc.creator.none.fl_str_mv Ugalde, R.A.
Coira, J.A.
Brill, W.J.
author Ugalde, R.A.
author_facet Ugalde, R.A.
Coira, J.A.
Brill, W.J.
author_role author
author2 Coira, J.A.
Brill, W.J.
author2_role author
author
dc.subject.none.fl_str_mv galactose
galacturonic acid
polysaccharide
uridine diphosphate galactose
uridine diphosphate glucuronic acid
article
nonhuman
priority journal
Sinorhizobium meliloti
Medicago sativa
Rhizobium
Scophthalmus rhombus
Sinorhizobium meliloti
topic galactose
galacturonic acid
polysaccharide
uridine diphosphate galactose
uridine diphosphate glucuronic acid
article
nonhuman
priority journal
Sinorhizobium meliloti
Medicago sativa
Rhizobium
Scophthalmus rhombus
Sinorhizobium meliloti
dc.description.none.fl_txt_mv Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.
Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.
publishDate 1986
dc.date.none.fl_str_mv 1986
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde
url http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. BACTERIOL. 1986;168(1):270-275
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1846784876180668416
score 12.982451

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