Biosynthesis of fatty acids and triacylglycerols by 2,6,10,14-tetramethyl pentadecane-grown cells of Nocardia globerula 432

• Autores: Alvarez, H.M.; Souto, M.F.; Viale, A.; Pucci, O.H.
• Año de publicación: 2001
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: FEMS Microbiol. Lett. 2001;200(2):195-200
• Materia: Fatty acid; Nocardia globerula; Pristane; Triacylglycerol; 2,6,10,14 tetramethyl pentadecane; 4,8,12 trimethyl tridecanoic acid; acrylic acid; alkane derivative; decanoic acid derivative; fatty acid derivative; propionyl coenzyme A; triacylglycerol; unclassified drug; Actinomyces; article; cell culture; controlled study; fatty acid oxidation; fatty acid synthesis; nocardia globerula; nonhuman; priority journal; Acetates; Acrylates; Carcinogens; Drug Interactions; Fatty Acids; Nocardia; Terpenes; Triglycerides
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Nocardia globerula strain 432 was able to synthesize triacylglycerols (TAG) during cultivation on 2,6,10,14-tetramethyl pentadecane (pristane) under nitrogen-limiting conditions. Within these cells, 4,8,12-trimethyl tridecanoic acid was the major fatty acid detected. Fatty acids with an odd number of carbon atoms and minor amounts of even-numbered fatty acids were also observed. Experiments carried out with acrylic acid, an inhibitor of β-oxidation, suggested that odd-numbered fatty acids such as C15:0, C17:0 and 10-methyl C17:0 were synthesized de novo using propionyl-CoA, the β-oxidation product, as precursor. Although N. globerula 432 incorporated mainly straight chain fatty acids into TAG, the branched fatty acid 4,8,12-trimethyl tridecanoic acid also appeared, to some extent, in the acylglycerols. The importance of TAG biosynthesis by pristane-grown cells of N. globerula strain 432 is discussed. © 2001 Federation of European Microbiological Societies.