Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion

Autores
Erickson, Bruce; Selvan, Senthamil Paramadayalan; Ko, Kerry W. S.; Kelly, Karen; Quiroga, Ariel Dario; Li, Lena; Nelson, Randy; King Jones, Kirst; Jacobs, René L.; Lehner, Richard
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.
Fil: Erickson, Bruce. University of Alberta; Canadá
Fil: Selvan, Senthamil Paramadayalan. University of Alberta; Canadá
Fil: Ko, Kerry W. S.. University of Alberta; Canadá
Fil: Kelly, Karen. University of Alberta; Canadá
Fil: Quiroga, Ariel Dario. University of Alberta; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Li, Lena. University of Alberta; Canadá
Fil: Nelson, Randy. University of Alberta; Canadá
Fil: King Jones, Kirst. University of Alberta; Canadá
Fil: Jacobs, René L.. University of Alberta; Canadá
Fil: Lehner, Richard. University of Alberta; Canadá
Materia
Hepatic Lipase
Triacylglycerol
Vldl
Apob
Fatty Acid Oxidation
Endoplasmic Reticulum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6036
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6036
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretionErickson, BruceSelvan, Senthamil ParamadayalanKo, Kerry W. S.Kelly, KarenQuiroga, Ariel DarioLi, LenaNelson, RandyKing Jones, KirstJacobs, René L.Lehner, RichardHepatic LipaseTriacylglycerolVldlApobFatty Acid OxidationEndoplasmic Reticulumhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.Fil: Erickson, Bruce. University of Alberta; CanadáFil: Selvan, Senthamil Paramadayalan. University of Alberta; CanadáFil: Ko, Kerry W. S.. University of Alberta; CanadáFil: Kelly, Karen. University of Alberta; CanadáFil: Quiroga, Ariel Dario. University of Alberta; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Li, Lena. University of Alberta; CanadáFil: Nelson, Randy. University of Alberta; CanadáFil: King Jones, Kirst. University of Alberta; CanadáFil: Jacobs, René L.. University of Alberta; CanadáFil: Lehner, Richard. University of Alberta; CanadáElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6036Erickson, Bruce; Selvan, Senthamil Paramadayalan; Ko, Kerry W. S.; Kelly, Karen; Quiroga, Ariel Dario; et al.; Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion; Elsevier; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1831; 6; 6-2013; 1113-11231388-1981enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138819811300036Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2013.01.017info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:51Zoai:ri.conicet.gov.ar:11336/6036instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:51.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
title Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
spellingShingle Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
Erickson, Bruce
Hepatic Lipase
Triacylglycerol
Vldl
Apob
Fatty Acid Oxidation
Endoplasmic Reticulum
title_short Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
title_full Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
title_fullStr Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
title_full_unstemmed Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
title_sort Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
dc.creator.none.fl_str_mv Erickson, Bruce
Selvan, Senthamil Paramadayalan
Ko, Kerry W. S.
Kelly, Karen
Quiroga, Ariel Dario
Li, Lena
Nelson, Randy
King Jones, Kirst
Jacobs, René L.
Lehner, Richard
author Erickson, Bruce
author_facet Erickson, Bruce
Selvan, Senthamil Paramadayalan
Ko, Kerry W. S.
Kelly, Karen
Quiroga, Ariel Dario
Li, Lena
Nelson, Randy
King Jones, Kirst
Jacobs, René L.
Lehner, Richard
author_role author
author2 Selvan, Senthamil Paramadayalan
Ko, Kerry W. S.
Kelly, Karen
Quiroga, Ariel Dario
Li, Lena
Nelson, Randy
King Jones, Kirst
Jacobs, René L.
Lehner, Richard
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hepatic Lipase
Triacylglycerol
Vldl
Apob
Fatty Acid Oxidation
Endoplasmic Reticulum
topic Hepatic Lipase
Triacylglycerol
Vldl
Apob
Fatty Acid Oxidation
Endoplasmic Reticulum
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.
Fil: Erickson, Bruce. University of Alberta; Canadá
Fil: Selvan, Senthamil Paramadayalan. University of Alberta; Canadá
Fil: Ko, Kerry W. S.. University of Alberta; Canadá
Fil: Kelly, Karen. University of Alberta; Canadá
Fil: Quiroga, Ariel Dario. University of Alberta; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Li, Lena. University of Alberta; Canadá
Fil: Nelson, Randy. University of Alberta; Canadá
Fil: King Jones, Kirst. University of Alberta; Canadá
Fil: Jacobs, René L.. University of Alberta; Canadá
Fil: Lehner, Richard. University of Alberta; Canadá
description Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6036
Erickson, Bruce; Selvan, Senthamil Paramadayalan; Ko, Kerry W. S.; Kelly, Karen; Quiroga, Ariel Dario; et al.; Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion; Elsevier; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1831; 6; 6-2013; 1113-1123
1388-1981
url http://hdl.handle.net/11336/6036
identifier_str_mv Erickson, Bruce; Selvan, Senthamil Paramadayalan; Ko, Kerry W. S.; Kelly, Karen; Quiroga, Ariel Dario; et al.; Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion; Elsevier; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1831; 6; 6-2013; 1113-1123
1388-1981
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138819811300036X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2013.01.017
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269977655115776
score 13.13397

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