Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021

Autores
Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Garcia de Lema, Mirta
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has been reported that Ensifer meliloti presents a high proportion of monounsaturated fatty acids and has a putative desaturase gene designated as PhFAD12 (National Centre for Biotechnology Information), encoding a putative Δ12 desaturase-like protein. In this work, we report the desaturation capacity and characterisation of this gene encoding the putative fatty acid desaturase of E. meliloti 1021. This gene was also isolated from the rhizobial strain and overexpressed in Escherichia coli. Compared to a control, the expression of this gene in the transformed strain decreased the levels of palmitic and stearic acids, enhanced palmitoleic and cis-vaccenic levels, and allowed for the detection of oleic acid. E. coli overexpressing the putative desaturase gene was capable of desaturating palmitic and stearic acids to monounsaturated fatty acids, similarly to the rhizobial strain. Our studies show that AAK64726 encodes a Δ9 desaturase instead of a Δ12 desaturase as previously indicated. This work describes evidence for the presence of a desaturase-mediated mechanism in monounsaturated fatty acid synthesis in E. meliloti 1021, which is modified by high growth temperature. This mechanism supplements the anaerobic mechanism for unsaturated fatty acid synthesis.
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Dardanelli, Marta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Garcia de Lema, Mirta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Materia
Fatty Acid Composition
Unsaturated Fatty Acids
Aerobic Mechanism
Desaturase Gene
Δ9 Fatty Acid Desaturase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34072
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021Paulucci, Natalia SoledadDardanelli, Marta SusanaGarcia de Lema, MirtaFatty Acid CompositionUnsaturated Fatty AcidsAerobic MechanismDesaturase GeneΔ9 Fatty Acid Desaturasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has been reported that Ensifer meliloti presents a high proportion of monounsaturated fatty acids and has a putative desaturase gene designated as PhFAD12 (National Centre for Biotechnology Information), encoding a putative Δ12 desaturase-like protein. In this work, we report the desaturation capacity and characterisation of this gene encoding the putative fatty acid desaturase of E. meliloti 1021. This gene was also isolated from the rhizobial strain and overexpressed in Escherichia coli. Compared to a control, the expression of this gene in the transformed strain decreased the levels of palmitic and stearic acids, enhanced palmitoleic and cis-vaccenic levels, and allowed for the detection of oleic acid. E. coli overexpressing the putative desaturase gene was capable of desaturating palmitic and stearic acids to monounsaturated fatty acids, similarly to the rhizobial strain. Our studies show that AAK64726 encodes a Δ9 desaturase instead of a Δ12 desaturase as previously indicated. This work describes evidence for the presence of a desaturase-mediated mechanism in monounsaturated fatty acid synthesis in E. meliloti 1021, which is modified by high growth temperature. This mechanism supplements the anaerobic mechanism for unsaturated fatty acid synthesis.Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Dardanelli, Marta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Garcia de Lema, Mirta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier Gmbh2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34072Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Garcia de Lema, Mirta; Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021; Elsevier Gmbh; Microbiological Research; 169; 5-6; 5-2014; 463-4680944-5013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2013.08.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944501313001286info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:57Zoai:ri.conicet.gov.ar:11336/34072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:57.756CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
title Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
spellingShingle Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
Paulucci, Natalia Soledad
Fatty Acid Composition
Unsaturated Fatty Acids
Aerobic Mechanism
Desaturase Gene
Δ9 Fatty Acid Desaturase
title_short Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
title_full Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
title_fullStr Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
title_full_unstemmed Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
title_sort Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021
dc.creator.none.fl_str_mv Paulucci, Natalia Soledad
Dardanelli, Marta Susana
Garcia de Lema, Mirta
author Paulucci, Natalia Soledad
author_facet Paulucci, Natalia Soledad
Dardanelli, Marta Susana
Garcia de Lema, Mirta
author_role author
author2 Dardanelli, Marta Susana
Garcia de Lema, Mirta
author2_role author
author
dc.subject.none.fl_str_mv Fatty Acid Composition
Unsaturated Fatty Acids
Aerobic Mechanism
Desaturase Gene
Δ9 Fatty Acid Desaturase
topic Fatty Acid Composition
Unsaturated Fatty Acids
Aerobic Mechanism
Desaturase Gene
Δ9 Fatty Acid Desaturase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It has been reported that Ensifer meliloti presents a high proportion of monounsaturated fatty acids and has a putative desaturase gene designated as PhFAD12 (National Centre for Biotechnology Information), encoding a putative Δ12 desaturase-like protein. In this work, we report the desaturation capacity and characterisation of this gene encoding the putative fatty acid desaturase of E. meliloti 1021. This gene was also isolated from the rhizobial strain and overexpressed in Escherichia coli. Compared to a control, the expression of this gene in the transformed strain decreased the levels of palmitic and stearic acids, enhanced palmitoleic and cis-vaccenic levels, and allowed for the detection of oleic acid. E. coli overexpressing the putative desaturase gene was capable of desaturating palmitic and stearic acids to monounsaturated fatty acids, similarly to the rhizobial strain. Our studies show that AAK64726 encodes a Δ9 desaturase instead of a Δ12 desaturase as previously indicated. This work describes evidence for the presence of a desaturase-mediated mechanism in monounsaturated fatty acid synthesis in E. meliloti 1021, which is modified by high growth temperature. This mechanism supplements the anaerobic mechanism for unsaturated fatty acid synthesis.
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Dardanelli, Marta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Garcia de Lema, Mirta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
description It has been reported that Ensifer meliloti presents a high proportion of monounsaturated fatty acids and has a putative desaturase gene designated as PhFAD12 (National Centre for Biotechnology Information), encoding a putative Δ12 desaturase-like protein. In this work, we report the desaturation capacity and characterisation of this gene encoding the putative fatty acid desaturase of E. meliloti 1021. This gene was also isolated from the rhizobial strain and overexpressed in Escherichia coli. Compared to a control, the expression of this gene in the transformed strain decreased the levels of palmitic and stearic acids, enhanced palmitoleic and cis-vaccenic levels, and allowed for the detection of oleic acid. E. coli overexpressing the putative desaturase gene was capable of desaturating palmitic and stearic acids to monounsaturated fatty acids, similarly to the rhizobial strain. Our studies show that AAK64726 encodes a Δ9 desaturase instead of a Δ12 desaturase as previously indicated. This work describes evidence for the presence of a desaturase-mediated mechanism in monounsaturated fatty acid synthesis in E. meliloti 1021, which is modified by high growth temperature. This mechanism supplements the anaerobic mechanism for unsaturated fatty acid synthesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34072
Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Garcia de Lema, Mirta; Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021; Elsevier Gmbh; Microbiological Research; 169; 5-6; 5-2014; 463-468
0944-5013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34072
identifier_str_mv Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Garcia de Lema, Mirta; Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021; Elsevier Gmbh; Microbiological Research; 169; 5-6; 5-2014; 463-468
0944-5013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2013.08.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944501313001286
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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