UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography

Autores
Bultel, L.; Landoni, M.; Grand, E.; Couto, A.S.; Kovensky, J.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.
Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Am. Soc. Mass Spectrom. 2010;21(1):178-190
Materia
Controlled degradation
Cyano-4-hydroxycinnamic acids
Dihydroxybenzoic acids
Harmane
High-performance anion-exchange chromatography
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix assisted laser desorption/ionization
New tools
Nitrous acid
Structural determination
Sulfate groups
Chromatographic analysis
Chromatography
Degradation
Desorption
Inorganic acids
Mass spectrometry
Oligosaccharides
Polysaccharides
Structural analysis
Ultraviolet lasers
Negative ions
alpha cyano 4 hydroxycinnamic acid
benzoic acid derivative
beta carboline
dihydroxybenzoic acid
heparin
nitrous acid
oligosaccharide
sulfate
unclassified drug
anion exchange chromatography
article
carbohydrate analysis
chemical structure
matrix assisted laser desorption ionization time of flight mass spectrometry
purification
structure analysis
ultraviolet spectroscopy
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Heparin
Molecular Sequence Data
Molecular Structure
Nitrous Acid
Oligosaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Time Factors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_10440305_v21_n1_p178_Bultel
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oai_identifier_str paperaa:paper_10440305_v21_n1_p178_Bultel
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange ChromatographyBultel, L.Landoni, M.Grand, E.Couto, A.S.Kovensky, J.Controlled degradationCyano-4-hydroxycinnamic acidsDihydroxybenzoic acidsHarmaneHigh-performance anion-exchange chromatographyMALDI-TOFMALDI-TOF mass spectrometryMatrix assisted laser desorption/ionizationNew toolsNitrous acidStructural determinationSulfate groupsChromatographic analysisChromatographyDegradationDesorptionInorganic acidsMass spectrometryOligosaccharidesPolysaccharidesStructural analysisUltraviolet lasersNegative ionsalpha cyano 4 hydroxycinnamic acidbenzoic acid derivativebeta carbolinedihydroxybenzoic acidheparinnitrous acidoligosaccharidesulfateunclassified druganion exchange chromatographyarticlecarbohydrate analysischemical structurematrix assisted laser desorption ionization time of flight mass spectrometrypurificationstructure analysisultraviolet spectroscopyCarbohydrate SequenceChromatography, High Pressure LiquidChromatography, Ion ExchangeHeparinMolecular Sequence DataMolecular StructureNitrous AcidOligosaccharidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletTime FactorsNitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_BultelJ. Am. Soc. Mass Spectrom. 2010;21(1):178-190reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:03Zpaperaa:paper_10440305_v21_n1_p178_BultelInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:04.386Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
title UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
spellingShingle UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
Bultel, L.
Controlled degradation
Cyano-4-hydroxycinnamic acids
Dihydroxybenzoic acids
Harmane
High-performance anion-exchange chromatography
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix assisted laser desorption/ionization
New tools
Nitrous acid
Structural determination
Sulfate groups
Chromatographic analysis
Chromatography
Degradation
Desorption
Inorganic acids
Mass spectrometry
Oligosaccharides
Polysaccharides
Structural analysis
Ultraviolet lasers
Negative ions
alpha cyano 4 hydroxycinnamic acid
benzoic acid derivative
beta carboline
dihydroxybenzoic acid
heparin
nitrous acid
oligosaccharide
sulfate
unclassified drug
anion exchange chromatography
article
carbohydrate analysis
chemical structure
matrix assisted laser desorption ionization time of flight mass spectrometry
purification
structure analysis
ultraviolet spectroscopy
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Heparin
Molecular Sequence Data
Molecular Structure
Nitrous Acid
Oligosaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Time Factors
title_short UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
title_full UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
title_fullStr UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
title_full_unstemmed UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
title_sort UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
dc.creator.none.fl_str_mv Bultel, L.
Landoni, M.
Grand, E.
Couto, A.S.
Kovensky, J.
author Bultel, L.
author_facet Bultel, L.
Landoni, M.
Grand, E.
Couto, A.S.
Kovensky, J.
author_role author
author2 Landoni, M.
Grand, E.
Couto, A.S.
Kovensky, J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Controlled degradation
Cyano-4-hydroxycinnamic acids
Dihydroxybenzoic acids
Harmane
High-performance anion-exchange chromatography
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix assisted laser desorption/ionization
New tools
Nitrous acid
Structural determination
Sulfate groups
Chromatographic analysis
Chromatography
Degradation
Desorption
Inorganic acids
Mass spectrometry
Oligosaccharides
Polysaccharides
Structural analysis
Ultraviolet lasers
Negative ions
alpha cyano 4 hydroxycinnamic acid
benzoic acid derivative
beta carboline
dihydroxybenzoic acid
heparin
nitrous acid
oligosaccharide
sulfate
unclassified drug
anion exchange chromatography
article
carbohydrate analysis
chemical structure
matrix assisted laser desorption ionization time of flight mass spectrometry
purification
structure analysis
ultraviolet spectroscopy
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Heparin
Molecular Sequence Data
Molecular Structure
Nitrous Acid
Oligosaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Time Factors
topic Controlled degradation
Cyano-4-hydroxycinnamic acids
Dihydroxybenzoic acids
Harmane
High-performance anion-exchange chromatography
MALDI-TOF
MALDI-TOF mass spectrometry
Matrix assisted laser desorption/ionization
New tools
Nitrous acid
Structural determination
Sulfate groups
Chromatographic analysis
Chromatography
Degradation
Desorption
Inorganic acids
Mass spectrometry
Oligosaccharides
Polysaccharides
Structural analysis
Ultraviolet lasers
Negative ions
alpha cyano 4 hydroxycinnamic acid
benzoic acid derivative
beta carboline
dihydroxybenzoic acid
heparin
nitrous acid
oligosaccharide
sulfate
unclassified drug
anion exchange chromatography
article
carbohydrate analysis
chemical structure
matrix assisted laser desorption ionization time of flight mass spectrometry
purification
structure analysis
ultraviolet spectroscopy
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Heparin
Molecular Sequence Data
Molecular Structure
Nitrous Acid
Oligosaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Time Factors
dc.description.none.fl_txt_mv Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.
Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_Bultel
url http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_Bultel
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Am. Soc. Mass Spectrom. 2010;21(1):178-190
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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