Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry...

Autores
Fontana, Ariel Ramón; Camargo, Alejandra Beatriz; Altamirano, Jorgelina Cecilia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE)is proposed for the first time for extracting and preconcentrating organophosphates pesticides (OPPs) from honey samples prior to gas chromatography-mass spectrometry (GC-MS) analysis.The extraction/preconcentration technique is supported on the micellar organized medium based on non-ionic surfactant. To enable coupling the proposed technique with GC, it was required to back extract the analytes into hexane. Several variables including, surfactant type and concentration, equilibration temperature and time, matrix modifiers, pH and buffers nature were studied and optimized over the relative response of the analytes. The best working conditions were as follows: an aliquot of 10 mL 50 g L-1 honey blend solution was conditioned by adding 100 µL 0.1 mol L-1 hydrochloric acid (pH 2) and finally extracted with 100 µL Triton X-114 100 g L-1 at 85 °C for 5 min by using CME technique. Under optimal experimental conditions, the enrichment factor (EF) was 167 and limits of detection (LODs), calculated as three times the signal-to-noise ratio (S/N=3), ranged between 0.03 ng g-1 and 0.47 ng g-1. The method precision was evaluated over five replicates at 1 ng g-1 with RSDs lower than 9.5%.The calibration graphs were linear within the concentration range of 0.3-1000 ng g-1 for Chlorpirifos; and 1-1000 ng g-1 for Fenitrothion, Parathion and Methidathion; respectively. The coefficients of correlation were higher than 0.9992.Validation of the methodology was performed by standard addition method at two concentration levels (2 and 20 ng g-1). The recoveries were higher than 90%, indicating satisfactory robustness of the methodology, which could be successfully applied for determination of OPPs in honey samples of different Argentinean regions. Two of the analyzed samples showed levels of methidathion ranged between 1.2 to 2.3 ng g-1.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Camargo, Alejandra Beatriz. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Enológicas y Agroalimentarias. Laboratorio de Residuos Tóxicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina
Materia
COACERVATIVE MICROEXTRACTION
ULTRASOUND-ASSISTED BACK-EXTRACTION
ORGANOPHOSPHATES PESTICIDES
HONEY ANALYSIS
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106679
Acceder
id CONICETDig_dc482ea6e9a53a612566d406dba9ab3a
oai_identifier_str oai:ri.conicet.gov.ar:11336/106679
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometryFontana, Ariel RamónCamargo, Alejandra BeatrizAltamirano, Jorgelina CeciliaCOACERVATIVE MICROEXTRACTIONULTRASOUND-ASSISTED BACK-EXTRACTIONORGANOPHOSPHATES PESTICIDESHONEY ANALYSISGAS CHROMATOGRAPHY-MASS SPECTROMETRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE)is proposed for the first time for extracting and preconcentrating organophosphates pesticides (OPPs) from honey samples prior to gas chromatography-mass spectrometry (GC-MS) analysis.The extraction/preconcentration technique is supported on the micellar organized medium based on non-ionic surfactant. To enable coupling the proposed technique with GC, it was required to back extract the analytes into hexane. Several variables including, surfactant type and concentration, equilibration temperature and time, matrix modifiers, pH and buffers nature were studied and optimized over the relative response of the analytes. The best working conditions were as follows: an aliquot of 10 mL 50 g L-1 honey blend solution was conditioned by adding 100 µL 0.1 mol L-1 hydrochloric acid (pH 2) and finally extracted with 100 µL Triton X-114 100 g L-1 at 85 °C for 5 min by using CME technique. Under optimal experimental conditions, the enrichment factor (EF) was 167 and limits of detection (LODs), calculated as three times the signal-to-noise ratio (S/N=3), ranged between 0.03 ng g-1 and 0.47 ng g-1. The method precision was evaluated over five replicates at 1 ng g-1 with RSDs lower than 9.5%.The calibration graphs were linear within the concentration range of 0.3-1000 ng g-1 for Chlorpirifos; and 1-1000 ng g-1 for Fenitrothion, Parathion and Methidathion; respectively. The coefficients of correlation were higher than 0.9992.Validation of the methodology was performed by standard addition method at two concentration levels (2 and 20 ng g-1). The recoveries were higher than 90%, indicating satisfactory robustness of the methodology, which could be successfully applied for determination of OPPs in honey samples of different Argentinean regions. Two of the analyzed samples showed levels of methidathion ranged between 1.2 to 2.3 ng g-1.Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Camargo, Alejandra Beatriz. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Enológicas y Agroalimentarias. Laboratorio de Residuos Tóxicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; ArgentinaElsevier Science2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106679Fontana, Ariel Ramón; Camargo, Alejandra Beatriz; Altamirano, Jorgelina Cecilia; Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry; Elsevier Science; Journal of Chromatography - A; 1217; 41; 10-2010; 6334-63410021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021967310010733info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2010.08.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:14Zoai:ri.conicet.gov.ar:11336/106679instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:14.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
title Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
spellingShingle Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
Fontana, Ariel Ramón
COACERVATIVE MICROEXTRACTION
ULTRASOUND-ASSISTED BACK-EXTRACTION
ORGANOPHOSPHATES PESTICIDES
HONEY ANALYSIS
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
title_short Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
title_full Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
title_fullStr Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
title_full_unstemmed Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
title_sort Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry
dc.creator.none.fl_str_mv Fontana, Ariel Ramón
Camargo, Alejandra Beatriz
Altamirano, Jorgelina Cecilia
author Fontana, Ariel Ramón
author_facet Fontana, Ariel Ramón
Camargo, Alejandra Beatriz
Altamirano, Jorgelina Cecilia
author_role author
author2 Camargo, Alejandra Beatriz
Altamirano, Jorgelina Cecilia
author2_role author
author
dc.subject.none.fl_str_mv COACERVATIVE MICROEXTRACTION
ULTRASOUND-ASSISTED BACK-EXTRACTION
ORGANOPHOSPHATES PESTICIDES
HONEY ANALYSIS
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
topic COACERVATIVE MICROEXTRACTION
ULTRASOUND-ASSISTED BACK-EXTRACTION
ORGANOPHOSPHATES PESTICIDES
HONEY ANALYSIS
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE)is proposed for the first time for extracting and preconcentrating organophosphates pesticides (OPPs) from honey samples prior to gas chromatography-mass spectrometry (GC-MS) analysis.The extraction/preconcentration technique is supported on the micellar organized medium based on non-ionic surfactant. To enable coupling the proposed technique with GC, it was required to back extract the analytes into hexane. Several variables including, surfactant type and concentration, equilibration temperature and time, matrix modifiers, pH and buffers nature were studied and optimized over the relative response of the analytes. The best working conditions were as follows: an aliquot of 10 mL 50 g L-1 honey blend solution was conditioned by adding 100 µL 0.1 mol L-1 hydrochloric acid (pH 2) and finally extracted with 100 µL Triton X-114 100 g L-1 at 85 °C for 5 min by using CME technique. Under optimal experimental conditions, the enrichment factor (EF) was 167 and limits of detection (LODs), calculated as three times the signal-to-noise ratio (S/N=3), ranged between 0.03 ng g-1 and 0.47 ng g-1. The method precision was evaluated over five replicates at 1 ng g-1 with RSDs lower than 9.5%.The calibration graphs were linear within the concentration range of 0.3-1000 ng g-1 for Chlorpirifos; and 1-1000 ng g-1 for Fenitrothion, Parathion and Methidathion; respectively. The coefficients of correlation were higher than 0.9992.Validation of the methodology was performed by standard addition method at two concentration levels (2 and 20 ng g-1). The recoveries were higher than 90%, indicating satisfactory robustness of the methodology, which could be successfully applied for determination of OPPs in honey samples of different Argentinean regions. Two of the analyzed samples showed levels of methidathion ranged between 1.2 to 2.3 ng g-1.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Camargo, Alejandra Beatriz. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Enológicas y Agroalimentarias. Laboratorio de Residuos Tóxicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina
description Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE)is proposed for the first time for extracting and preconcentrating organophosphates pesticides (OPPs) from honey samples prior to gas chromatography-mass spectrometry (GC-MS) analysis.The extraction/preconcentration technique is supported on the micellar organized medium based on non-ionic surfactant. To enable coupling the proposed technique with GC, it was required to back extract the analytes into hexane. Several variables including, surfactant type and concentration, equilibration temperature and time, matrix modifiers, pH and buffers nature were studied and optimized over the relative response of the analytes. The best working conditions were as follows: an aliquot of 10 mL 50 g L-1 honey blend solution was conditioned by adding 100 µL 0.1 mol L-1 hydrochloric acid (pH 2) and finally extracted with 100 µL Triton X-114 100 g L-1 at 85 °C for 5 min by using CME technique. Under optimal experimental conditions, the enrichment factor (EF) was 167 and limits of detection (LODs), calculated as three times the signal-to-noise ratio (S/N=3), ranged between 0.03 ng g-1 and 0.47 ng g-1. The method precision was evaluated over five replicates at 1 ng g-1 with RSDs lower than 9.5%.The calibration graphs were linear within the concentration range of 0.3-1000 ng g-1 for Chlorpirifos; and 1-1000 ng g-1 for Fenitrothion, Parathion and Methidathion; respectively. The coefficients of correlation were higher than 0.9992.Validation of the methodology was performed by standard addition method at two concentration levels (2 and 20 ng g-1). The recoveries were higher than 90%, indicating satisfactory robustness of the methodology, which could be successfully applied for determination of OPPs in honey samples of different Argentinean regions. Two of the analyzed samples showed levels of methidathion ranged between 1.2 to 2.3 ng g-1.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106679
Fontana, Ariel Ramón; Camargo, Alejandra Beatriz; Altamirano, Jorgelina Cecilia; Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry; Elsevier Science; Journal of Chromatography - A; 1217; 41; 10-2010; 6334-6341
0021-9673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106679
identifier_str_mv Fontana, Ariel Ramón; Camargo, Alejandra Beatriz; Altamirano, Jorgelina Cecilia; Coacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography–mass spectrometry; Elsevier Science; Journal of Chromatography - A; 1217; 41; 10-2010; 6334-6341
0021-9673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021967310010733
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2010.08.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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