Selection of early-occurring mutations dictates hormone-independent progression in mouse mammary tumor lines

• Autores: Gattelli, A.; Zimberlin, M.N.; Meiss, R.P.; Castilla, L.H.; Kordon, E.C.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. Virol. 2006;80(22):11409-11415
• Materia: animal tissue; article; breast cancer; controlled study; female; gene insertion; gene mutation; gene overexpression; int7 gene; mouse; Mouse mammary tumor oncovirus; nonhuman; nucleotide sequence; pregnancy; priority journal; reverse transcription polymerase chain reaction; rspo3 gene; tumor cell line; tumor growth; virus gene; Animals; Disease Models, Animal; Female; Mammary Neoplasms, Experimental; Mammary Tumor Virus, Mouse; Mice; Mutation; Recombination, Genetic; Retroviridae Infections; Selection (Genetics); Tumor Virus Infections; Mouse mammary tumor virus
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Mice harboring three mouse mammary tumor virus (MMTV) variants develop pregnancy-dependent (PD) tumors that progress to pregnancy-independent (PI) behavior through successive passages. Herein, we identified 10 predominant insertions in PI transplants from 8 independent tumor lines. These mutations were also detected in small cell populations in the early PD passages. In addition, we identified a new viral insertion upstream of the gene Rspo3, which is overexpressed in three of the eight independent tumor lines and codes for a protein very similar to the recently described protein encoded by Int7. This study suggests that during progression towards hormone independence, clonal expansion of cells with specific mutations might be more relevant than the occurrence of new MMTV insertions. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.