First insight into Mycobacterium tuberculosis genetic diversity in Paraguay

Autores
Candia, Norma; López, Beatriz; Zozio, Thierry; Carrivale, Marcela; Diaz, Chyntia; Russomando, Graciela; de Romero, Nilda J.; Jara, Juan C.; Barrera, Lucía; Rastogi, Nalin; Ritacco, Viviana
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results: A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpoIDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpoIDB4. Conclusion: Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe.
Fil: Candia, Norma. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Zozio, Thierry. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.
Fil: Carrivale, Marcela.ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Diaz, Chyntia. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: Russomando, Graciela. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: de Romero, Nilda J. Laboratorio Central de Salud Pública; Paraguay.
Fil: Jara, Juan C. Programa Nacional de Control de la Tuberculosis; Paraguay.
Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Rastogi, Nalin. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fuente
BMC Microbiology, 2007, 7(75).
Materia
Mycobacterium tuberculosis
Tuberculosis
Paraguay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/414
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/414
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling First insight into Mycobacterium tuberculosis genetic diversity in ParaguayCandia, NormaLópez, BeatrizZozio, ThierryCarrivale, MarcelaDiaz, ChyntiaRussomando, Gracielade Romero, Nilda J.Jara, Juan C.Barrera, LucíaRastogi, NalinRitacco, VivianaMycobacterium tuberculosisTuberculosisParaguayBackground: We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results: A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpoIDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpoIDB4. Conclusion: Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe.Fil: Candia, Norma. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.Fil: Zozio, Thierry. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.Fil: Carrivale, Marcela.ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.Fil: Diaz, Chyntia. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.Fil: Russomando, Graciela. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.Fil: de Romero, Nilda J. Laboratorio Central de Salud Pública; Paraguay.Fil: Jara, Juan C. Programa Nacional de Control de la Tuberculosis; Paraguay.Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.Fil: Rastogi, Nalin. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.2007info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1471-2180http://www.biomedcentral.com/content/pdf/1471-2180-7-75.pdfhttp://sgc.anlis.gob.ar/handle/123456789/414BMC Microbiology, 2007, 7(75).reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISPARenginfo:eu-repo/semantics/openAccess2025-09-04T11:15:51Zoai:sgc.anlis.gob.ar:Publications/123456789/414Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:15:51.441Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
title First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
spellingShingle First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
Candia, Norma
Mycobacterium tuberculosis
Tuberculosis
Paraguay
title_short First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
title_full First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
title_fullStr First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
title_full_unstemmed First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
title_sort First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
dc.creator.none.fl_str_mv Candia, Norma
López, Beatriz
Zozio, Thierry
Carrivale, Marcela
Diaz, Chyntia
Russomando, Graciela
de Romero, Nilda J.
Jara, Juan C.
Barrera, Lucía
Rastogi, Nalin
Ritacco, Viviana
author Candia, Norma
author_facet Candia, Norma
López, Beatriz
Zozio, Thierry
Carrivale, Marcela
Diaz, Chyntia
Russomando, Graciela
de Romero, Nilda J.
Jara, Juan C.
Barrera, Lucía
Rastogi, Nalin
Ritacco, Viviana
author_role author
author2 López, Beatriz
Zozio, Thierry
Carrivale, Marcela
Diaz, Chyntia
Russomando, Graciela
de Romero, Nilda J.
Jara, Juan C.
Barrera, Lucía
Rastogi, Nalin
Ritacco, Viviana
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium tuberculosis
Tuberculosis
Paraguay
topic Mycobacterium tuberculosis
Tuberculosis
Paraguay
dc.description.none.fl_txt_mv Background: We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results: A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpoIDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpoIDB4. Conclusion: Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe.
Fil: Candia, Norma. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Zozio, Thierry. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.
Fil: Carrivale, Marcela.ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Diaz, Chyntia. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: Russomando, Graciela. Universidad Nacional de Asunción. Departamento de Biología Molecular; Paraguay.
Fil: de Romero, Nilda J. Laboratorio Central de Salud Pública; Paraguay.
Fil: Jara, Juan C. Programa Nacional de Control de la Tuberculosis; Paraguay.
Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
Fil: Rastogi, Nalin. Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobacteries; Francia.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Biología Molecular; Argentina.
description Background: We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results: A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpoIDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpoIDB4. Conclusion: Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1471-2180
http://www.biomedcentral.com/content/pdf/1471-2180-7-75.pdf
http://sgc.anlis.gob.ar/handle/123456789/414
identifier_str_mv 1471-2180
url http://www.biomedcentral.com/content/pdf/1471-2180-7-75.pdf
http://sgc.anlis.gob.ar/handle/123456789/414
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv PAR
dc.source.none.fl_str_mv BMC Microbiology, 2007, 7(75).
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
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instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
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repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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