Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

Autores
Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; De Berrêdo Pinho Moreira, Marcia; De Romero, Nilda J.; Morcillo, Nora Susana; De Waard, Jacobus Henri; Gomes, Harrison Magdinier; Suffys, Philip Noel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.
Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; Brasil
Fil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; Brasil
Fil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; Paraguay
Fil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; Argentina
Fil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; Ecuador
Fil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; Brasil
Fil: Suffys, Philip Noel. Fundación Oswaldo Cruz; Brasil
Materia
GENOTYPING
LAM
MYCOBACTERIUM TUBERCULOSIS
PARAGUAY
SOUTH-AMERICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123164
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/123164
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and VenezuelaDíaz Acosta, Chyntia CarolinaRussomando, Graciela MabelCandia, NormaRitacco, Gloria VivianaVasconcellos, Sidra E. G.De Berrêdo Pinho Moreira, MarciaDe Romero, Nilda J.Morcillo, Nora SusanaDe Waard, Jacobus HenriGomes, Harrison MagdinierSuffys, Philip NoelGENOTYPINGLAMMYCOBACTERIUM TUBERCULOSISPARAGUAYSOUTH-AMERICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; BrasilFil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; BrasilFil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; ParaguayFil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; ArgentinaFil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; EcuadorFil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; BrasilFil: Suffys, Philip Noel. Fundación Oswaldo Cruz; BrasilBioMed Central2019-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123164Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-131471-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1479-6info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-019-1479-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:28Zoai:ri.conicet.gov.ar:11336/123164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:28.962CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
title Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
spellingShingle Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
Díaz Acosta, Chyntia Carolina
GENOTYPING
LAM
MYCOBACTERIUM TUBERCULOSIS
PARAGUAY
SOUTH-AMERICA
title_short Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
title_full Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
title_fullStr Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
title_full_unstemmed Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
title_sort Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
dc.creator.none.fl_str_mv Díaz Acosta, Chyntia Carolina
Russomando, Graciela Mabel
Candia, Norma
Ritacco, Gloria Viviana
Vasconcellos, Sidra E. G.
De Berrêdo Pinho Moreira, Marcia
De Romero, Nilda J.
Morcillo, Nora Susana
De Waard, Jacobus Henri
Gomes, Harrison Magdinier
Suffys, Philip Noel
author Díaz Acosta, Chyntia Carolina
author_facet Díaz Acosta, Chyntia Carolina
Russomando, Graciela Mabel
Candia, Norma
Ritacco, Gloria Viviana
Vasconcellos, Sidra E. G.
De Berrêdo Pinho Moreira, Marcia
De Romero, Nilda J.
Morcillo, Nora Susana
De Waard, Jacobus Henri
Gomes, Harrison Magdinier
Suffys, Philip Noel
author_role author
author2 Russomando, Graciela Mabel
Candia, Norma
Ritacco, Gloria Viviana
Vasconcellos, Sidra E. G.
De Berrêdo Pinho Moreira, Marcia
De Romero, Nilda J.
Morcillo, Nora Susana
De Waard, Jacobus Henri
Gomes, Harrison Magdinier
Suffys, Philip Noel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENOTYPING
LAM
MYCOBACTERIUM TUBERCULOSIS
PARAGUAY
SOUTH-AMERICA
topic GENOTYPING
LAM
MYCOBACTERIUM TUBERCULOSIS
PARAGUAY
SOUTH-AMERICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.
Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; Brasil
Fil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; Brasil
Fil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; Paraguay
Fil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; Argentina
Fil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; Ecuador
Fil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; Brasil
Fil: Suffys, Philip Noel. Fundación Oswaldo Cruz; Brasil
description Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123164
Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-13
1471-2180
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123164
identifier_str_mv Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-13
1471-2180
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-019-1479-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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