Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela
- Autores
- Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; De Berrêdo Pinho Moreira, Marcia; De Romero, Nilda J.; Morcillo, Nora Susana; De Waard, Jacobus Henri; Gomes, Harrison Magdinier; Suffys, Philip Noel
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.
Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay
Fil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; Brasil
Fil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; Brasil
Fil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; Paraguay
Fil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; Argentina
Fil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; Ecuador
Fil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; Brasil
Fil: Suffys, Philip Noel. Fundación Oswaldo Cruz; Brasil - Materia
-
GENOTYPING
LAM
MYCOBACTERIUM TUBERCULOSIS
PARAGUAY
SOUTH-AMERICA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/123164
Ver los metadatos del registro completo
id |
CONICETDig_6a94afc87c1bd98cac8d0af1a47546cf |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/123164 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and VenezuelaDíaz Acosta, Chyntia CarolinaRussomando, Graciela MabelCandia, NormaRitacco, Gloria VivianaVasconcellos, Sidra E. G.De Berrêdo Pinho Moreira, MarciaDe Romero, Nilda J.Morcillo, Nora SusanaDe Waard, Jacobus HenriGomes, Harrison MagdinierSuffys, Philip NoelGENOTYPINGLAMMYCOBACTERIUM TUBERCULOSISPARAGUAYSOUTH-AMERICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; ParaguayFil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; BrasilFil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; BrasilFil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; ParaguayFil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; ArgentinaFil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; EcuadorFil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; BrasilFil: Suffys, Philip Noel. Fundación Oswaldo Cruz; BrasilBioMed Central2019-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123164Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-131471-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1479-6info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-019-1479-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:28Zoai:ri.conicet.gov.ar:11336/123164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:28.962CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
title |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
spellingShingle |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela Díaz Acosta, Chyntia Carolina GENOTYPING LAM MYCOBACTERIUM TUBERCULOSIS PARAGUAY SOUTH-AMERICA |
title_short |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
title_full |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
title_fullStr |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
title_full_unstemmed |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
title_sort |
Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela |
dc.creator.none.fl_str_mv |
Díaz Acosta, Chyntia Carolina Russomando, Graciela Mabel Candia, Norma Ritacco, Gloria Viviana Vasconcellos, Sidra E. G. De Berrêdo Pinho Moreira, Marcia De Romero, Nilda J. Morcillo, Nora Susana De Waard, Jacobus Henri Gomes, Harrison Magdinier Suffys, Philip Noel |
author |
Díaz Acosta, Chyntia Carolina |
author_facet |
Díaz Acosta, Chyntia Carolina Russomando, Graciela Mabel Candia, Norma Ritacco, Gloria Viviana Vasconcellos, Sidra E. G. De Berrêdo Pinho Moreira, Marcia De Romero, Nilda J. Morcillo, Nora Susana De Waard, Jacobus Henri Gomes, Harrison Magdinier Suffys, Philip Noel |
author_role |
author |
author2 |
Russomando, Graciela Mabel Candia, Norma Ritacco, Gloria Viviana Vasconcellos, Sidra E. G. De Berrêdo Pinho Moreira, Marcia De Romero, Nilda J. Morcillo, Nora Susana De Waard, Jacobus Henri Gomes, Harrison Magdinier Suffys, Philip Noel |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
GENOTYPING LAM MYCOBACTERIUM TUBERCULOSIS PARAGUAY SOUTH-AMERICA |
topic |
GENOTYPING LAM MYCOBACTERIUM TUBERCULOSIS PARAGUAY SOUTH-AMERICA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries. Fil: Díaz Acosta, Chyntia Carolina. Fundación Oswaldo Cruz; Brasil. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay Fil: Candia, Norma. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay Fil: Ritacco, Gloria Viviana. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vasconcellos, Sidra E. G.. Fundación Oswaldo Cruz; Brasil Fil: De Berrêdo Pinho Moreira, Marcia. Fundación Oswaldo Cruz; Brasil Fil: De Romero, Nilda J.. Laboratorio Central de Salud Pública; Paraguay Fil: Morcillo, Nora Susana. Instituto Nacional de Enfermedades Respiratorias Emilio Coni; Argentina Fil: De Waard, Jacobus Henri. Instituto de Biomedicina. Laboratorio de Tuberculosis; Venezuela. Universidad de Las Américas.; Ecuador Fil: Gomes, Harrison Magdinier. Fundación Oswaldo Cruz; Brasil Fil: Suffys, Philip Noel. Fundación Oswaldo Cruz; Brasil |
description |
Background: The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results: Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions: Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-13 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/123164 Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-13 1471-2180 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/123164 |
identifier_str_mv |
Díaz Acosta, Chyntia Carolina; Russomando, Graciela Mabel; Candia, Norma; Ritacco, Gloria Viviana; Vasconcellos, Sidra E. G.; et al.; Exploring the "latin American Mediterranean" family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela; BioMed Central; BMC Microbiology; 19; 1; 13-6-2019; 1-13 1471-2180 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1479-6 info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-019-1479-6 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846083089207394304 |
score |
13.22299 |