Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders

Autores
Fellner, María Dolores; Durand, Karina A; Solernou, Veronica; Bosaleh, Andrea; Balbarrey, Ziomara; García de Dávila, María T; Rodriguez, Marcelo; Irazu, Lucía; Alonio, Lidia V.; Picconi, María Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed.
Fuente
Revista Argentina de Microbiología 2016; 48(2):110-118
Materia
Carga viral
Linfoma
Reacción en Cadena en Tiempo Real de la Polimerasa
Herpesvirus Humano 4
Niño
Preescolar
Estudios Transversales
ADN Viral
Detección Precoz del Cáncer
Infecciones por Virus de Epstein-Barr
Estudios de Seguimiento
Humanos
Huésped Inmunocomprometido
Lactante
Leucocitos Mononucleares
Tejido Linfoide
Linfoma
Trastornos Linfoproliferativos
Complicaciones Posoperatorias
Estudios Retrospectivos
Viremia
Trasplante de Corazón
Trasplante de Riñón
Trasplante de Hígado
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/1864

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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/1864
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disordersCarga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplanteFellner, María DoloresDurand, Karina ASolernou, VeronicaBosaleh, AndreaBalbarrey, ZiomaraGarcía de Dávila, María TRodriguez, MarceloIrazu, LucíaAlonio, Lidia V.Picconi, María AlejandraCarga viralLinfomaReacción en Cadena en Tiempo Real de la PolimerasaHerpesvirus Humano 4NiñoPreescolarEstudios TransversalesADN ViralDetección Precoz del CáncerInfecciones por Virus de Epstein-BarrEstudios de SeguimientoHumanosHuésped InmunocomprometidoLactanteLeucocitos MononuclearesTejido LinfoideLinfomaTrastornos LinfoproliferativosComplicaciones PosoperatoriasEstudios RetrospectivosViremiaTrasplante de CorazónTrasplante de RiñónTrasplante de HígadoFil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed.Asociación Argentina de Microbiología2016-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0325-7541http://sgc.anlis.gob.ar/handle/123456789/186410.1016/j.ram.2016.02.006Revista Argentina de Microbiología 2016; 48(2):110-118reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsRevista Argentina de microbiologiaenginfo:eu-repo/semantics/openAccess2025-09-29T14:30:28Zoai:sgc.anlis.gob.ar:123456789/1864Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:28.824Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
Carga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplante
title Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
spellingShingle Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
Fellner, María Dolores
Carga viral
Linfoma
Reacción en Cadena en Tiempo Real de la Polimerasa
Herpesvirus Humano 4
Niño
Preescolar
Estudios Transversales
ADN Viral
Detección Precoz del Cáncer
Infecciones por Virus de Epstein-Barr
Estudios de Seguimiento
Humanos
Huésped Inmunocomprometido
Lactante
Leucocitos Mononucleares
Tejido Linfoide
Linfoma
Trastornos Linfoproliferativos
Complicaciones Posoperatorias
Estudios Retrospectivos
Viremia
Trasplante de Corazón
Trasplante de Riñón
Trasplante de Hígado
title_short Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
title_full Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
title_fullStr Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
title_full_unstemmed Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
title_sort Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
dc.creator.none.fl_str_mv Fellner, María Dolores
Durand, Karina A
Solernou, Veronica
Bosaleh, Andrea
Balbarrey, Ziomara
García de Dávila, María T
Rodriguez, Marcelo
Irazu, Lucía
Alonio, Lidia V.
Picconi, María Alejandra
author Fellner, María Dolores
author_facet Fellner, María Dolores
Durand, Karina A
Solernou, Veronica
Bosaleh, Andrea
Balbarrey, Ziomara
García de Dávila, María T
Rodriguez, Marcelo
Irazu, Lucía
Alonio, Lidia V.
Picconi, María Alejandra
author_role author
author2 Durand, Karina A
Solernou, Veronica
Bosaleh, Andrea
Balbarrey, Ziomara
García de Dávila, María T
Rodriguez, Marcelo
Irazu, Lucía
Alonio, Lidia V.
Picconi, María Alejandra
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Carga viral
Linfoma
Reacción en Cadena en Tiempo Real de la Polimerasa
Herpesvirus Humano 4
Niño
Preescolar
Estudios Transversales
ADN Viral
Detección Precoz del Cáncer
Infecciones por Virus de Epstein-Barr
Estudios de Seguimiento
Humanos
Huésped Inmunocomprometido
Lactante
Leucocitos Mononucleares
Tejido Linfoide
Linfoma
Trastornos Linfoproliferativos
Complicaciones Posoperatorias
Estudios Retrospectivos
Viremia
Trasplante de Corazón
Trasplante de Riñón
Trasplante de Hígado
topic Carga viral
Linfoma
Reacción en Cadena en Tiempo Real de la Polimerasa
Herpesvirus Humano 4
Niño
Preescolar
Estudios Transversales
ADN Viral
Detección Precoz del Cáncer
Infecciones por Virus de Epstein-Barr
Estudios de Seguimiento
Humanos
Huésped Inmunocomprometido
Lactante
Leucocitos Mononucleares
Tejido Linfoide
Linfoma
Trastornos Linfoproliferativos
Complicaciones Posoperatorias
Estudios Retrospectivos
Viremia
Trasplante de Corazón
Trasplante de Riñón
Trasplante de Hígado
dc.description.none.fl_txt_mv Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed.
description Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0325-7541
http://sgc.anlis.gob.ar/handle/123456789/1864
10.1016/j.ram.2016.02.006
identifier_str_mv 0325-7541
10.1016/j.ram.2016.02.006
url http://sgc.anlis.gob.ar/handle/123456789/1864
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
datasets
Revista Argentina de microbiologia
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv Revista Argentina de Microbiología 2016; 48(2):110-118
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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