Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
- Autores
- Fellner, María Dolores; Durand, Karina A; Solernou, Veronica; Bosaleh, Andrea; Balbarrey, Ziomara; García de Dávila, María T; Rodriguez, Marcelo; Irazu, Lucía; Alonio, Lidia V.; Picconi, María Alejandra
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed. - Fuente
- Revista Argentina de Microbiología 2016; 48(2):110-118
- Materia
-
Carga viral
Linfoma
Reacción en Cadena en Tiempo Real de la Polimerasa
Herpesvirus Humano 4
Niño
Preescolar
Estudios Transversales
ADN Viral
Detección Precoz del Cáncer
Infecciones por Virus de Epstein-Barr
Estudios de Seguimiento
Humanos
Huésped Inmunocomprometido
Lactante
Leucocitos Mononucleares
Tejido Linfoide
Linfoma
Trastornos Linfoproliferativos
Complicaciones Posoperatorias
Estudios Retrospectivos
Viremia
Trasplante de Corazón
Trasplante de Riñón
Trasplante de Hígado - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/1864
Ver los metadatos del registro completo
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Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disordersCarga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplanteFellner, María DoloresDurand, Karina ASolernou, VeronicaBosaleh, AndreaBalbarrey, ZiomaraGarcía de Dávila, María TRodriguez, MarceloIrazu, LucíaAlonio, Lidia V.Picconi, María AlejandraCarga viralLinfomaReacción en Cadena en Tiempo Real de la PolimerasaHerpesvirus Humano 4NiñoPreescolarEstudios TransversalesADN ViralDetección Precoz del CáncerInfecciones por Virus de Epstein-BarrEstudios de SeguimientoHumanosHuésped InmunocomprometidoLactanteLeucocitos MononuclearesTejido LinfoideLinfomaTrastornos LinfoproliferativosComplicaciones PosoperatoriasEstudios RetrospectivosViremiaTrasplante de CorazónTrasplante de RiñónTrasplante de HígadoFil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed.Asociación Argentina de Microbiología2016-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0325-7541http://sgc.anlis.gob.ar/handle/123456789/186410.1016/j.ram.2016.02.006Revista Argentina de Microbiología 2016; 48(2):110-118reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsRevista Argentina de microbiologiaenginfo:eu-repo/semantics/openAccess2025-09-29T14:30:28Zoai:sgc.anlis.gob.ar:123456789/1864Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:28.824Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders Carga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplante |
title |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
spellingShingle |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders Fellner, María Dolores Carga viral Linfoma Reacción en Cadena en Tiempo Real de la Polimerasa Herpesvirus Humano 4 Niño Preescolar Estudios Transversales ADN Viral Detección Precoz del Cáncer Infecciones por Virus de Epstein-Barr Estudios de Seguimiento Humanos Huésped Inmunocomprometido Lactante Leucocitos Mononucleares Tejido Linfoide Linfoma Trastornos Linfoproliferativos Complicaciones Posoperatorias Estudios Retrospectivos Viremia Trasplante de Corazón Trasplante de Riñón Trasplante de Hígado |
title_short |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
title_full |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
title_fullStr |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
title_full_unstemmed |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
title_sort |
Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders |
dc.creator.none.fl_str_mv |
Fellner, María Dolores Durand, Karina A Solernou, Veronica Bosaleh, Andrea Balbarrey, Ziomara García de Dávila, María T Rodriguez, Marcelo Irazu, Lucía Alonio, Lidia V. Picconi, María Alejandra |
author |
Fellner, María Dolores |
author_facet |
Fellner, María Dolores Durand, Karina A Solernou, Veronica Bosaleh, Andrea Balbarrey, Ziomara García de Dávila, María T Rodriguez, Marcelo Irazu, Lucía Alonio, Lidia V. Picconi, María Alejandra |
author_role |
author |
author2 |
Durand, Karina A Solernou, Veronica Bosaleh, Andrea Balbarrey, Ziomara García de Dávila, María T Rodriguez, Marcelo Irazu, Lucía Alonio, Lidia V. Picconi, María Alejandra |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Carga viral Linfoma Reacción en Cadena en Tiempo Real de la Polimerasa Herpesvirus Humano 4 Niño Preescolar Estudios Transversales ADN Viral Detección Precoz del Cáncer Infecciones por Virus de Epstein-Barr Estudios de Seguimiento Humanos Huésped Inmunocomprometido Lactante Leucocitos Mononucleares Tejido Linfoide Linfoma Trastornos Linfoproliferativos Complicaciones Posoperatorias Estudios Retrospectivos Viremia Trasplante de Corazón Trasplante de Riñón Trasplante de Hígado |
topic |
Carga viral Linfoma Reacción en Cadena en Tiempo Real de la Polimerasa Herpesvirus Humano 4 Niño Preescolar Estudios Transversales ADN Viral Detección Precoz del Cáncer Infecciones por Virus de Epstein-Barr Estudios de Seguimiento Humanos Huésped Inmunocomprometido Lactante Leucocitos Mononucleares Tejido Linfoide Linfoma Trastornos Linfoproliferativos Complicaciones Posoperatorias Estudios Retrospectivos Viremia Trasplante de Corazón Trasplante de Riñón Trasplante de Hígado |
dc.description.none.fl_txt_mv |
Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed. |
description |
Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
0325-7541 http://sgc.anlis.gob.ar/handle/123456789/1864 10.1016/j.ram.2016.02.006 |
identifier_str_mv |
0325-7541 10.1016/j.ram.2016.02.006 |
url |
http://sgc.anlis.gob.ar/handle/123456789/1864 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# datasets Revista Argentina de microbiologia |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Asociación Argentina de Microbiología |
publisher.none.fl_str_mv |
Asociación Argentina de Microbiología |
dc.source.none.fl_str_mv |
Revista Argentina de Microbiología 2016; 48(2):110-118 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
reponame_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
collection |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
institution |
ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
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1844621857397932032 |
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12.559606 |