A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients
- Autores
- Fellner, María Dolores; Durand, Karina; Correa, Mariel; Bes, David; Alonio, Lidia V.; Teyssie, Angelica R.; Picconi, María Alejandra
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Fellner, María Dolores. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Fil: Durand, Karina. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Fil: Correa, Mariel. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Fil: Bes, David. Hospital Nacional de Pediatría "Prof. Juan P. Garrahan"; Buenos Aires, Argentina.
Fil: Alonio, Lidia V. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Fil: Teyssié, Angélica R. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Fil: Picconi, María Alejandra. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.
Background: High Epstein-Barr virus load has been related to an increased risk of Posttransplant Lymphoproliferative Disorders (PTLD) in transplant recipients. Objectives: Development of a method to quantitate EBV DNA levels in peripheral blood mononuclear cells (PBMC) and evaluate its usefulness in transplant patients. Study design: We designed a semiquantitative nested PCR based on a limiting dilution analysis to detect high viral loads in PBMC. This method was applied to 25 healthy carriers, and 85 solid organ transplant recipients as follows: (A) 53 asymptomatic patients; (B) 24 symptomatic patients; (C) eight patients with PTLD. Results: In healthy carriers the reciprocal of the limiting dilution (RLD) ranged between non-detected (ND) and 1, the median RLD was ND, which is equivalent to a viral load of <1 copy per 10(5) PBMC. In the transplant population the medians RLD (range) were: (A) asymptomatic group: ND (ND-64), median equivalent to a viral load of <1 copy per 10(5) PBMC; (B) symptomatic group: 4 (ND-256), median equivalent to a range of viral load of 4-64 copies per 10(5) PBMC. (C) PTLD group: 256 (16-16384), median equivalent to a range of viral load of 256-4096 copies per 10(5) PBMC. Statistically significant differences were found between all groups: A+B vs. C (P<0.0001); A vs. B (P<0.0001); A vs. C (P<0.0001), B vs. C (P<0.0001). We also observed a good correlation between viral loads and clinical findings in four follow-up patients. Considering the RLD=256 as a cutoff point to detect transplant patients with PTLD, resulted in sensitivity 75%, specificity 96.7%, positive predictive value 60%, negative predictive value 98.3%. Conclusion: This SQ-PCR method enables us to differentiate between transplant patients with and without PTLD; therefore, it could be applied as a marker for early detection of this pathology. - Materia
-
Trastornos Linfoproliferativos
Valor Predictivo de las Pruebas
Trasplante de Hígado
Carga Viral - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/1734
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patientsFellner, María DoloresDurand, KarinaCorrea, MarielBes, DavidAlonio, Lidia V.Teyssie, Angelica R.Picconi, María AlejandraTrastornos LinfoproliferativosValor Predictivo de las PruebasTrasplante de HígadoCarga ViralFil: Fellner, María Dolores. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Fil: Durand, Karina. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Fil: Correa, Mariel. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Fil: Bes, David. Hospital Nacional de Pediatría "Prof. Juan P. Garrahan"; Buenos Aires, Argentina.Fil: Alonio, Lidia V. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Fil: Teyssié, Angélica R. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Fil: Picconi, María Alejandra. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina.Background: High Epstein-Barr virus load has been related to an increased risk of Posttransplant Lymphoproliferative Disorders (PTLD) in transplant recipients. Objectives: Development of a method to quantitate EBV DNA levels in peripheral blood mononuclear cells (PBMC) and evaluate its usefulness in transplant patients. Study design: We designed a semiquantitative nested PCR based on a limiting dilution analysis to detect high viral loads in PBMC. This method was applied to 25 healthy carriers, and 85 solid organ transplant recipients as follows: (A) 53 asymptomatic patients; (B) 24 symptomatic patients; (C) eight patients with PTLD. Results: In healthy carriers the reciprocal of the limiting dilution (RLD) ranged between non-detected (ND) and 1, the median RLD was ND, which is equivalent to a viral load of <1 copy per 10(5) PBMC. In the transplant population the medians RLD (range) were: (A) asymptomatic group: ND (ND-64), median equivalent to a viral load of <1 copy per 10(5) PBMC; (B) symptomatic group: 4 (ND-256), median equivalent to a range of viral load of 4-64 copies per 10(5) PBMC. (C) PTLD group: 256 (16-16384), median equivalent to a range of viral load of 256-4096 copies per 10(5) PBMC. Statistically significant differences were found between all groups: A+B vs. C (P<0.0001); A vs. B (P<0.0001); A vs. C (P<0.0001), B vs. C (P<0.0001). We also observed a good correlation between viral loads and clinical findings in four follow-up patients. Considering the RLD=256 as a cutoff point to detect transplant patients with PTLD, resulted in sensitivity 75%, specificity 96.7%, positive predictive value 60%, negative predictive value 98.3%. Conclusion: This SQ-PCR method enables us to differentiate between transplant patients with and without PTLD; therefore, it could be applied as a marker for early detection of this pathology.2003-12info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1386-6532http://sgc.anlis.gob.ar/handle/123456789/173410.1016/s1386-6532(03)00077-5Journal of clinical virology : the official publication of the Pan American Society for Clinical Virologyenginfo:eu-repo/semantics/openAccessreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS2025-09-29T14:30:25Zoai:sgc.anlis.gob.ar:123456789/1734Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:25.913Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
title |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
spellingShingle |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients Fellner, María Dolores Trastornos Linfoproliferativos Valor Predictivo de las Pruebas Trasplante de Hígado Carga Viral |
title_short |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
title_full |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
title_fullStr |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
title_full_unstemmed |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
title_sort |
A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients |
dc.creator.none.fl_str_mv |
Fellner, María Dolores Durand, Karina Correa, Mariel Bes, David Alonio, Lidia V. Teyssie, Angelica R. Picconi, María Alejandra |
author |
Fellner, María Dolores |
author_facet |
Fellner, María Dolores Durand, Karina Correa, Mariel Bes, David Alonio, Lidia V. Teyssie, Angelica R. Picconi, María Alejandra |
author_role |
author |
author2 |
Durand, Karina Correa, Mariel Bes, David Alonio, Lidia V. Teyssie, Angelica R. Picconi, María Alejandra |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Trastornos Linfoproliferativos Valor Predictivo de las Pruebas Trasplante de Hígado Carga Viral |
topic |
Trastornos Linfoproliferativos Valor Predictivo de las Pruebas Trasplante de Hígado Carga Viral |
dc.description.none.fl_txt_mv |
Fil: Fellner, María Dolores. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Fil: Durand, Karina. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Fil: Correa, Mariel. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Fil: Bes, David. Hospital Nacional de Pediatría "Prof. Juan P. Garrahan"; Buenos Aires, Argentina. Fil: Alonio, Lidia V. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Fil: Teyssié, Angélica R. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Fil: Picconi, María Alejandra. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. Background: High Epstein-Barr virus load has been related to an increased risk of Posttransplant Lymphoproliferative Disorders (PTLD) in transplant recipients. Objectives: Development of a method to quantitate EBV DNA levels in peripheral blood mononuclear cells (PBMC) and evaluate its usefulness in transplant patients. Study design: We designed a semiquantitative nested PCR based on a limiting dilution analysis to detect high viral loads in PBMC. This method was applied to 25 healthy carriers, and 85 solid organ transplant recipients as follows: (A) 53 asymptomatic patients; (B) 24 symptomatic patients; (C) eight patients with PTLD. Results: In healthy carriers the reciprocal of the limiting dilution (RLD) ranged between non-detected (ND) and 1, the median RLD was ND, which is equivalent to a viral load of <1 copy per 10(5) PBMC. In the transplant population the medians RLD (range) were: (A) asymptomatic group: ND (ND-64), median equivalent to a viral load of <1 copy per 10(5) PBMC; (B) symptomatic group: 4 (ND-256), median equivalent to a range of viral load of 4-64 copies per 10(5) PBMC. (C) PTLD group: 256 (16-16384), median equivalent to a range of viral load of 256-4096 copies per 10(5) PBMC. Statistically significant differences were found between all groups: A+B vs. C (P<0.0001); A vs. B (P<0.0001); A vs. C (P<0.0001), B vs. C (P<0.0001). We also observed a good correlation between viral loads and clinical findings in four follow-up patients. Considering the RLD=256 as a cutoff point to detect transplant patients with PTLD, resulted in sensitivity 75%, specificity 96.7%, positive predictive value 60%, negative predictive value 98.3%. Conclusion: This SQ-PCR method enables us to differentiate between transplant patients with and without PTLD; therefore, it could be applied as a marker for early detection of this pathology. |
description |
Fil: Fellner, María Dolores. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1386-6532 http://sgc.anlis.gob.ar/handle/123456789/1734 10.1016/s1386-6532(03)00077-5 |
identifier_str_mv |
1386-6532 10.1016/s1386-6532(03)00077-5 |
url |
http://sgc.anlis.gob.ar/handle/123456789/1734 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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ANLIS |
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ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
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