Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
- Autores
- Carnevale, Silvana; Malandrini, Jorge Bruno; Pantano, María Laura; Sawicki, Mirna; Kamenetzky, Laura; Soria, Claudia Cecilia; Velásquez, Jorge Néstor
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.
Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections. - Fuente
- Acta Parasitológica 2020
- Materia
-
Ensayo de Inmunoadsorción Enzimática
Fascioliasis
Reacción en Cadena de la Polimerasa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- none
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/1759
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Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic AreaCarnevale, SilvanaMalandrini, Jorge BrunoPantano, María LauraSawicki, MirnaKamenetzky, LauraSoria, Claudia CeciliaVelásquez, Jorge NéstorEnsayo de Inmunoadsorción EnzimáticaFascioliasisReacción en Cadena de la PolimerasaFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.De GruyterInstitute of Parasitology2020-10-28info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://sgc.anlis.gob.ar/handle/123456789/175910.1007/s11686-020-00302-2Acta Parasitológica 2020reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsActa parasitologicanoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:25Zoai:sgc.anlis.gob.ar:123456789/1759Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:26.025Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
title |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
spellingShingle |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area Carnevale, Silvana Ensayo de Inmunoadsorción Enzimática Fascioliasis Reacción en Cadena de la Polimerasa |
title_short |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
title_full |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
title_fullStr |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
title_full_unstemmed |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
title_sort |
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area |
dc.creator.none.fl_str_mv |
Carnevale, Silvana Malandrini, Jorge Bruno Pantano, María Laura Sawicki, Mirna Kamenetzky, Laura Soria, Claudia Cecilia Velásquez, Jorge Néstor |
author |
Carnevale, Silvana |
author_facet |
Carnevale, Silvana Malandrini, Jorge Bruno Pantano, María Laura Sawicki, Mirna Kamenetzky, Laura Soria, Claudia Cecilia Velásquez, Jorge Néstor |
author_role |
author |
author2 |
Malandrini, Jorge Bruno Pantano, María Laura Sawicki, Mirna Kamenetzky, Laura Soria, Claudia Cecilia Velásquez, Jorge Néstor |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ensayo de Inmunoadsorción Enzimática Fascioliasis Reacción en Cadena de la Polimerasa |
topic |
Ensayo de Inmunoadsorción Enzimática Fascioliasis Reacción en Cadena de la Polimerasa |
dc.description.none.fl_txt_mv |
Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina. Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina. Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina. Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections. |
description |
Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-28 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sgc.anlis.gob.ar/handle/123456789/1759 10.1007/s11686-020-00302-2 |
url |
http://sgc.anlis.gob.ar/handle/123456789/1759 |
identifier_str_mv |
10.1007/s11686-020-00302-2 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# datasets Acta parasitologica |
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none info:eu-repo/semantics/openAccess |
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none |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
De Gruyter Institute of Parasitology |
publisher.none.fl_str_mv |
De Gruyter Institute of Parasitology |
dc.source.none.fl_str_mv |
Acta Parasitológica 2020 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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biblioteca@anlis.gov.ar |
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