Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area

Autores
Carnevale, Silvana; Malandrini, Jorge Bruno; Pantano, María Laura; Sawicki, Mirna; Kamenetzky, Laura; Soria, Claudia Cecilia; Velásquez, Jorge Néstor
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.
Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.
Fuente
Acta Parasitológica 2020
Materia
Ensayo de Inmunoadsorción Enzimática
Fascioliasis
Reacción en Cadena de la Polimerasa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1759

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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic AreaCarnevale, SilvanaMalandrini, Jorge BrunoPantano, María LauraSawicki, MirnaKamenetzky, LauraSoria, Claudia CeciliaVelásquez, Jorge NéstorEnsayo de Inmunoadsorción EnzimáticaFascioliasisReacción en Cadena de la PolimerasaFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.De GruyterInstitute of Parasitology2020-10-28info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://sgc.anlis.gob.ar/handle/123456789/175910.1007/s11686-020-00302-2Acta Parasitológica 2020reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsActa parasitologicanoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:25Zoai:sgc.anlis.gob.ar:Publications/123456789/1759Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:26.022Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
title Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
spellingShingle Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
Carnevale, Silvana
Ensayo de Inmunoadsorción Enzimática
Fascioliasis
Reacción en Cadena de la Polimerasa
title_short Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
title_full Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
title_fullStr Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
title_full_unstemmed Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
title_sort Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
dc.creator.none.fl_str_mv Carnevale, Silvana
Malandrini, Jorge Bruno
Pantano, María Laura
Sawicki, Mirna
Kamenetzky, Laura
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author Carnevale, Silvana
author_facet Carnevale, Silvana
Malandrini, Jorge Bruno
Pantano, María Laura
Sawicki, Mirna
Kamenetzky, Laura
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author_role author
author2 Malandrini, Jorge Bruno
Pantano, María Laura
Sawicki, Mirna
Kamenetzky, Laura
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ensayo de Inmunoadsorción Enzimática
Fascioliasis
Reacción en Cadena de la Polimerasa
topic Ensayo de Inmunoadsorción Enzimática
Fascioliasis
Reacción en Cadena de la Polimerasa
dc.description.none.fl_txt_mv Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.
Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.
Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.
description Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-28
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sgc.anlis.gob.ar/handle/123456789/1759
10.1007/s11686-020-00302-2
url http://sgc.anlis.gob.ar/handle/123456789/1759
identifier_str_mv 10.1007/s11686-020-00302-2
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
datasets
Acta parasitologica
dc.rights.none.fl_str_mv none
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rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv De Gruyter
Institute of Parasitology
publisher.none.fl_str_mv De Gruyter
Institute of Parasitology
dc.source.none.fl_str_mv Acta Parasitológica 2020
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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