Characterisation of a cyclophilin isoform in Trypanosoma cruzi

Autores
Bua, Jacqueline; Åslund, Lena; Pereyra, N; García, Gabriela Andrea; Bontempi, Esteban; Ruiz, Andrés Mariano
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Aslund, Lena. Rudbeck Laboratory. Department of Genetics and Pathology; Suecia.
Fil: Pereyra, Natalia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Bontempi, Esteban J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
The immunosuppressive drug cyclosporin A (CsA) has shown antiparasitic activity against several protozoans and helminths, when complexed to proteins called cyclophilins (CyPs). In this paper, the molecular characterisation of one member of the CyP family in Trypanosoma cruzi is reported. TcCyP19 gene proved to be highly conserved compared to CyPs from other organisms and was highly homologous to a Trypanosoma brucei brucei CyPA. This gene was expressed in Escherichia coli and the purified recombinant protein exhibited a peptidyl prolyl cis-trans isomerase activity that was inhibited by CsA (IC(50) = 18.4 + /-0.8 nM). The TcCyP19 gene was located on two chromosomal bands in T. cruzi CL Brener clone.
Materia
Enfermedad de Chagas
Ciclosporina
Escherichia coli
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/1507
Acceder
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Characterisation of a cyclophilin isoform in Trypanosoma cruziBua, JacquelineÅslund, LenaPereyra, NGarcía, Gabriela AndreaBontempi, EstebanRuiz, Andrés MarianoEnfermedad de ChagasCiclosporinaEscherichia coliFil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.Fil: Aslund, Lena. Rudbeck Laboratory. Department of Genetics and Pathology; Suecia.Fil: Pereyra, Natalia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.Fil: Bontempi, Esteban J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.The immunosuppressive drug cyclosporin A (CsA) has shown antiparasitic activity against several protozoans and helminths, when complexed to proteins called cyclophilins (CyPs). In this paper, the molecular characterisation of one member of the CyP family in Trypanosoma cruzi is reported. TcCyP19 gene proved to be highly conserved compared to CyPs from other organisms and was highly homologous to a Trypanosoma brucei brucei CyPA. This gene was expressed in Escherichia coli and the purified recombinant protein exhibited a peptidyl prolyl cis-trans isomerase activity that was inhibited by CsA (IC(50) = 18.4 + /-0.8 nM). The TcCyP19 gene was located on two chromosomal bands in T. cruzi CL Brener clone.2001-06-12info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0378-1097http://sgc.anlis.gob.ar/handle/123456789/1507https://academic.oup.com/femsle/article/200/1/43/55016310.1111/j.1574-6968.2001.tb10690.xFEMS microbiology lettersnoneinfo:eu-repo/semantics/openAccessengreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS2025-09-29T14:30:20Zoai:sgc.anlis.gob.ar:123456789/1507Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:20.891Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Characterisation of a cyclophilin isoform in Trypanosoma cruzi
title Characterisation of a cyclophilin isoform in Trypanosoma cruzi
spellingShingle Characterisation of a cyclophilin isoform in Trypanosoma cruzi
Bua, Jacqueline
Enfermedad de Chagas
Ciclosporina
Escherichia coli
title_short Characterisation of a cyclophilin isoform in Trypanosoma cruzi
title_full Characterisation of a cyclophilin isoform in Trypanosoma cruzi
title_fullStr Characterisation of a cyclophilin isoform in Trypanosoma cruzi
title_full_unstemmed Characterisation of a cyclophilin isoform in Trypanosoma cruzi
title_sort Characterisation of a cyclophilin isoform in Trypanosoma cruzi
dc.creator.none.fl_str_mv Bua, Jacqueline
Åslund, Lena
Pereyra, N
García, Gabriela Andrea
Bontempi, Esteban
Ruiz, Andrés Mariano
author Bua, Jacqueline
author_facet Bua, Jacqueline
Åslund, Lena
Pereyra, N
García, Gabriela Andrea
Bontempi, Esteban
Ruiz, Andrés Mariano
author_role author
author2 Åslund, Lena
Pereyra, N
García, Gabriela Andrea
Bontempi, Esteban
Ruiz, Andrés Mariano
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedad de Chagas
Ciclosporina
Escherichia coli
topic Enfermedad de Chagas
Ciclosporina
Escherichia coli
dc.description.none.fl_txt_mv Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Aslund, Lena. Rudbeck Laboratory. Department of Genetics and Pathology; Suecia.
Fil: Pereyra, Natalia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Bontempi, Esteban J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
The immunosuppressive drug cyclosporin A (CsA) has shown antiparasitic activity against several protozoans and helminths, when complexed to proteins called cyclophilins (CyPs). In this paper, the molecular characterisation of one member of the CyP family in Trypanosoma cruzi is reported. TcCyP19 gene proved to be highly conserved compared to CyPs from other organisms and was highly homologous to a Trypanosoma brucei brucei CyPA. This gene was expressed in Escherichia coli and the purified recombinant protein exhibited a peptidyl prolyl cis-trans isomerase activity that was inhibited by CsA (IC(50) = 18.4 + /-0.8 nM). The TcCyP19 gene was located on two chromosomal bands in T. cruzi CL Brener clone.
description Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
publishDate 2001
dc.date.none.fl_str_mv 2001-06-12
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0378-1097
http://sgc.anlis.gob.ar/handle/123456789/1507
https://academic.oup.com/femsle/article/200/1/43/550163
10.1111/j.1574-6968.2001.tb10690.x
identifier_str_mv 0378-1097
10.1111/j.1574-6968.2001.tb10690.x
url http://sgc.anlis.gob.ar/handle/123456789/1507
https://academic.oup.com/femsle/article/200/1/43/550163
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEMS microbiology letters
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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