Distribution of human papillomavirus (HPV) genotypes and bacterial vaginosis presence in cervical samples from Paraguayan indigenous

• Autores: Mongelos, Pamela; Mendoza, Laura Patricia; Rodriguez-Riveros, Isabel; Castro, Amalia; Gimenez, Graciela; Araujo, Patricia; Paez, Malvina; Castro, Wilberto; Basiletti, Jorge; González, Joaquín V.; Echagüe, Gloria; Diaz, Valentina; Laspina, Florentina; Ever, Santiago; Marecos, Ramón; Deluca, Gerardo; Picconi, María Alejandra
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: International Journal of Infectious Diseases 2015; 39:44-49
• Materia: Vaginosis Bacteriana; Alphapapillomavirus; Paraguay; Cuello del Útero; Chlamydia trachomatis; Adulto; Coinfección; Genotipo; Papillomavirus Humano 16; Humanos; Mycoplasma hominis; Papillomaviridae; Infecciones por Papillomavirus; Trichomonas vaginalis; Adulto Joven
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Mongelos, Pamela. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Mendoza, Laura Patricia. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Rodríguez-Riveros, Isabel. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Castro, Amalia. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Giménez, Graciela. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Araujo, Patricia. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Paez, Malvina. National University of Asuncion. Health Sciences Research Institute. Department of Public Health; Paraguay.
Fil: Castro, Wilberto. National University of Asuncion. Faculty of Medical Sciences. Cervical Pathology Department; Paraguay.
Fil: Basiletti, Jorge. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: González, Joaquín. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Echagüe, Gloria. National University of Asuncion. Department of Clinical and microbiological analysis; Paraguay.
Fil: Diaz, Valentina. National University of Asuncion. Department of Clinical and microbiological analysis; Paraguay.
Fil: Laspina, Florentina. National University of Asuncion. Department of Clinical and microbiological analysis; Paraguay.
Fil: Ever, Santiago. Ministry of Public Health. Regional Hospital of Villa Hayes; Paraguay.
Fil: Marecos, Ramón. Ministry of Public Health. Health Center of Pozo Colorado; Paraguay.
Fil: Deluca, Gerardo. Northeast National University Corrientes. Faculty of Medicine. Molecular Applications Laboratory; Argentina.
Fil: Picconi, María Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Objective: To determine the frequency of human papillomavirus (HPV) types and to assess bacterial vaginosis (BV) possible associations with cervical infections in indigenous Paraguayan women of the Department of Presidente Hayes. Methods: This study included 181 sexually active women without cervical lesions. HPV typing was performed by polymerase chain reaction with primers PGMY 09/11 followed by reverse line hybridization. BV was diagnosed by the Nugent criteria using the results from a Gram stain smear. Results: Sixteen percent of women were positive for at least one high risk HPV type (HR-HPV). The most frequent genotypes were HPV 16 (4.4%), followed by HPV 58 (3.3%), HPV 45 (3.3%), HPV 53 (2.8%) and HPV 11 (2.8%). A significant association between HR-HPV and BV was observed (p=0.01). In addition, women with BV had a higher frequency of Chlamydia trachomatis (p=0.0007), Trichomonas vaginalis (p=0.00009), Mycoplasma hominis (p=0.001). Conclusions: A large variety of HPV genotypes was detected and showed a slightly different pattern from previous studies on urban women in Paraguay, with the predominance of HR-HPV. Furthermore, the information of co-infections involved in BV could be useful for the improvement of national prevention programs, as well as for laboratory surveillance of these genital infections.