A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms

Autores
de Roodt, Adolfo R.; Dolab, Jorge A.; Dokmetjian, Christian; Litwin, Silvana; Segré, Liliana; Vidal, Juan C.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Segré, Liliana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
Fil: Vidal, Juan C. Museo Argentino de Ciencias Naturales Bernardino Rivadavia. Area lología; Argentina.
The hemorrhagic activity of Bothrops (B.) alternatus, B. ammodytoides, B. jararaca, B. jararacussu, B. moojeni and B. neuwiedii venoms from specimens captured in Argentina was assayed after i.d. injection to mice. The hemorrhagic haloes produced by each venom had different color intensities, although no significant differences were observed by measurement of the average diameters or the weight of the excised hemorrhagic haloes. Conversely, important differences were found by measuring the amount of hemoglobin extracted from excised hemorrhagic haloes of similar size produced by different venoms. The relationship between the amount of hemoglobin extracted and the weight of the excised hemorrhagic haloes was linear, with a slope (hemoglobin released per gram of hemorrhagic halo) characteristic for each venom, and proportional to the potency. On this basis, the activity of B. alternatus, B. ammodytoides and B. jararaca is similar, about 1.5 times higher than that of B. jararacussu and B. moojeni venoms and threefold higher than that of B. neuwiedii venom. Thus, measurement of the of hemoglobin released provides additional information in comparative studies, and may be used to assess the antihemorrhagic potency of antivenoms.
Materia
Venenos de Serpiente
Hemorragia
Antivenenos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/1697
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/1697
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling A comparison of different methods to assess the hemorrhagic activity of Bothrops venomsde Roodt, Adolfo R.Dolab, Jorge A.Dokmetjian, ChristianLitwin, SilvanaSegré, LilianaVidal, Juan C.Venenos de SerpienteHemorragiaAntivenenosFil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; ArgentinaFil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Segré, Liliana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; ArgentinaFil: Vidal, Juan C. Museo Argentino de Ciencias Naturales Bernardino Rivadavia. Area lología; Argentina.The hemorrhagic activity of Bothrops (B.) alternatus, B. ammodytoides, B. jararaca, B. jararacussu, B. moojeni and B. neuwiedii venoms from specimens captured in Argentina was assayed after i.d. injection to mice. The hemorrhagic haloes produced by each venom had different color intensities, although no significant differences were observed by measurement of the average diameters or the weight of the excised hemorrhagic haloes. Conversely, important differences were found by measuring the amount of hemoglobin extracted from excised hemorrhagic haloes of similar size produced by different venoms. The relationship between the amount of hemoglobin extracted and the weight of the excised hemorrhagic haloes was linear, with a slope (hemoglobin released per gram of hemorrhagic halo) characteristic for each venom, and proportional to the potency. On this basis, the activity of B. alternatus, B. ammodytoides and B. jararaca is similar, about 1.5 times higher than that of B. jararacussu and B. moojeni venoms and threefold higher than that of B. neuwiedii venom. Thus, measurement of the of hemoglobin released provides additional information in comparative studies, and may be used to assess the antihemorrhagic potency of antivenoms.2000-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0041-0101http://sgc.anlis.gob.ar/handle/123456789/1697https://doi.org/10.1016/S0041-0101(99)00205-6Toxicon : official journal of the International Society on Toxinologynoneinfo:eu-repo/semantics/openAccessengreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS2025-09-04T11:17:20Zoai:sgc.anlis.gob.ar:123456789/1697Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:17:21.118Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
title A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
spellingShingle A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
de Roodt, Adolfo R.
Venenos de Serpiente
Hemorragia
Antivenenos
title_short A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
title_full A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
title_fullStr A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
title_full_unstemmed A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
title_sort A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
dc.creator.none.fl_str_mv de Roodt, Adolfo R.
Dolab, Jorge A.
Dokmetjian, Christian
Litwin, Silvana
Segré, Liliana
Vidal, Juan C.
author de Roodt, Adolfo R.
author_facet de Roodt, Adolfo R.
Dolab, Jorge A.
Dokmetjian, Christian
Litwin, Silvana
Segré, Liliana
Vidal, Juan C.
author_role author
author2 Dolab, Jorge A.
Dokmetjian, Christian
Litwin, Silvana
Segré, Liliana
Vidal, Juan C.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Venenos de Serpiente
Hemorragia
Antivenenos
topic Venenos de Serpiente
Hemorragia
Antivenenos
dc.description.none.fl_txt_mv Fil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Segré, Liliana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
Fil: Vidal, Juan C. Museo Argentino de Ciencias Naturales Bernardino Rivadavia. Area lología; Argentina.
The hemorrhagic activity of Bothrops (B.) alternatus, B. ammodytoides, B. jararaca, B. jararacussu, B. moojeni and B. neuwiedii venoms from specimens captured in Argentina was assayed after i.d. injection to mice. The hemorrhagic haloes produced by each venom had different color intensities, although no significant differences were observed by measurement of the average diameters or the weight of the excised hemorrhagic haloes. Conversely, important differences were found by measuring the amount of hemoglobin extracted from excised hemorrhagic haloes of similar size produced by different venoms. The relationship between the amount of hemoglobin extracted and the weight of the excised hemorrhagic haloes was linear, with a slope (hemoglobin released per gram of hemorrhagic halo) characteristic for each venom, and proportional to the potency. On this basis, the activity of B. alternatus, B. ammodytoides and B. jararaca is similar, about 1.5 times higher than that of B. jararacussu and B. moojeni venoms and threefold higher than that of B. neuwiedii venom. Thus, measurement of the of hemoglobin released provides additional information in comparative studies, and may be used to assess the antihemorrhagic potency of antivenoms.
description Fil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
publishDate 2000
dc.date.none.fl_str_mv 2000-06
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0041-0101
http://sgc.anlis.gob.ar/handle/123456789/1697
https://doi.org/10.1016/S0041-0101(99)00205-6
identifier_str_mv 0041-0101
url http://sgc.anlis.gob.ar/handle/123456789/1697
https://doi.org/10.1016/S0041-0101(99)00205-6
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon : official journal of the International Society on Toxinology
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.623145

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