Epstein Barr virus genotypes and LMP-1 variants in HIV-infected patients

• Autores: Correa, Rita Mariel; Fellner, María Dolores; Durand, Karina; Redini, Liliana; Alonio, Virginia; Yampolsky, Claudio; Colobraro, Antonio; Sevlever, Gustavo; Teyssie, Angelica R.; Benetucci, Jorge; Picconi, María Alejandra
• Año de publicación: 2007
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of Medical Virology 2007; 79(4):401-407
• Materia: Síndrome de Inmunodeficiencia Adquirida; Adolescente; Adulto; Argentina; Secuencia de Bases; Biopsia; Southern Blotting; Encéfalo; Neoplasias del Sistema Nervioso Central; Infecciones por Virus de Epstein-Barr; Variación Genética; Infecciones por VIH; Herpesvirus Humano 4; Humanos; Leucocitos Mononucleares; Linfoma Relacionado con SIDA; Masculino; Persona de Mediana Edad; Reacción en Cadena de la Polimerasa; Eliminación de Secuencia; Especificidad de la Especie; Proteínas de la Matriz Viral; VIH
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Correa, Rita Mariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Durand, Karina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Redini, Liliana. Hospital de Enfermedades Infecciosas “Dr. F. Muñiz”; Argentina.
Fil: Alonio, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Yampolsky, Claudio. Sanatorio Güemes. Servicio de Neurocirugía; Argentina.
Fil: Colobraro, Antonio. Clínica Bazterrica. Servicio Patología; Argentina.
Fil: Sevlever, Gustavo. Instituto Fleni. Servicio Patología; Argentina.
Fil: Teyssié, Angélica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Benetucci, Jorge. Fundación Ayuda al Inmunodeficiente. Laboratorio de Retrovirus y virus asociados; Argentina.
Fil: Picconi, María Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Two Epstein Barr virus (EBV) genotypes: EBV-1 and EBV-2 have been described. A 30-bp deletion in latent membrane protein-1 gene (del-LMP-1) has been identified in various pathologies. The aim of this study was to determine EBV genotypes and 30-bp deletion frequency in HIV-infected patients from Argentina. The study was performed on 258 individuals: Cases: 144 HIV-infected patients that included: (a) 7 AIDS patients with primary central nervous system lymphoma (PCNSL), (b) 62 AIDS patients, and (c) 75 asymptomatic HIV-infected patients. Controls: 114 HIV-negative individuals. EBV genotypes and variants in LMP-1 gene were detected by polymerase chain reaction (PCR)-Southern blot on DNA extracted from peripheral blood mononuclear cells and brain biopsies. In PCNSL, the presence of EBV was confirmed by EBER RNA in situ hybridization, and DNA sequencing of 3' end LMP-l gene of PCR products was performed. In HIV-infected patients, EBV-1 was detected in 48.6%, EBV-2 in 18.8%, and co-infection with both genotypes in 32.6%. In control group, EBV-1 was present in 74.3%, EBV-2 in 12.4%, and co-infection in 13.3%. Del-LMP-1 was found in 44.4% of HIV-infected patients samples (20.7% alone and 23.7% co-infection with non-deleted form) while it was found in 25.3% (6.3% alone and 19% with co-infection) in HIV-negative individuals. In HIV-infected patients EBV-2, co-infection and 30-bp deletion are more prevalent than in control group. In all, PCNSL brain biopsies samples, del-LMP-1 always was detected with EBV-2, but more cases would have to be included to draw definitive conclusions.