Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina

Autores
Cortes, Paulo R.; Albarracín Orio, Andrea G.; Regueira, Mabel; Piñas, Germán E.; Echenique, José
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Cortes, Paulo R. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Albarracín Orio, Andrea G.Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Regueira, Mabel. ANLIS Dr.C.G.Malbrán.Instituto Nacional de Enfermedades Infecciosas. Servicio de Bacteriología Clínica; Argentina.
Fil: Piñas, Germán E. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Echenique, José. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains.
Fuente
Journal of Clinical Microbiology, 2008, 46(6), 1930–1934.
Materia
Streptococcus pneumoniae
Serotipificación
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/270

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spelling Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from ArgentinaCortes, Paulo R.Albarracín Orio, Andrea G.Regueira, MabelPiñas, Germán E.Echenique, JoséStreptococcus pneumoniaeSerotipificaciónArgentinaFil: Cortes, Paulo R. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.Fil: Albarracín Orio, Andrea G.Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.Fil: Regueira, Mabel. ANLIS Dr.C.G.Malbrán.Instituto Nacional de Enfermedades Infecciosas. Servicio de Bacteriología Clínica; Argentina.Fil: Piñas, Germán E. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.Fil: Echenique, José. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains.2008info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-660Xhttp://sgc.anlis.gob.ar/handle/123456789/270http://jcm.asm.org/content/46/6/1930.full.pdf+htmlJournal of Clinical Microbiology, 2008, 46(6), 1930–1934.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of clinical microbiologyARGnoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:29:55Zoai:sgc.anlis.gob.ar:Publications/123456789/270Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:29:55.455Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
title Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
spellingShingle Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
Cortes, Paulo R.
Streptococcus pneumoniae
Serotipificación
Argentina
title_short Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
title_full Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
title_fullStr Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
title_full_unstemmed Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
title_sort Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
dc.creator.none.fl_str_mv Cortes, Paulo R.
Albarracín Orio, Andrea G.
Regueira, Mabel
Piñas, Germán E.
Echenique, José
author Cortes, Paulo R.
author_facet Cortes, Paulo R.
Albarracín Orio, Andrea G.
Regueira, Mabel
Piñas, Germán E.
Echenique, José
author_role author
author2 Albarracín Orio, Andrea G.
Regueira, Mabel
Piñas, Germán E.
Echenique, José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Streptococcus pneumoniae
Serotipificación
Argentina
topic Streptococcus pneumoniae
Serotipificación
Argentina
dc.description.none.fl_txt_mv Fil: Cortes, Paulo R. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Albarracín Orio, Andrea G.Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Regueira, Mabel. ANLIS Dr.C.G.Malbrán.Instituto Nacional de Enfermedades Infecciosas. Servicio de Bacteriología Clínica; Argentina.
Fil: Piñas, Germán E. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Fil: Echenique, José. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains.
description Fil: Cortes, Paulo R. Universidad Nacional de Córdoba. Departamento de Bioquímica Clínica; Argentina.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-660X
http://sgc.anlis.gob.ar/handle/123456789/270
http://jcm.asm.org/content/46/6/1930.full.pdf+html
identifier_str_mv 1098-660X
url http://sgc.anlis.gob.ar/handle/123456789/270
http://jcm.asm.org/content/46/6/1930.full.pdf+html
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of clinical microbiology
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv ARG
dc.source.none.fl_str_mv Journal of Clinical Microbiology, 2008, 46(6), 1930–1934.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
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instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
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repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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