Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina
- Autores
- Cortes, Paulo; Albarracín Orio, Andrea Georgina; Regueira, Mabel; Piñas, German Eduardo; Echenique, Jose Ricardo
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains.
Fil: Cortes, Paulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Albarracín Orio, Andrea Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina - Materia
-
STREPTOCOCCUS PNEUMONIAE
H+-ATPASE
OPTOQUIN RESISTANCE
atpAC GENES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/241124
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Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from ArgentinaCortes, PauloAlbarracín Orio, Andrea GeorginaRegueira, MabelPiñas, German EduardoEchenique, Jose RicardoSTREPTOCOCCUS PNEUMONIAEH+-ATPASEOPTOQUIN RESISTANCEatpAC GENEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains.Fil: Cortes, Paulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Albarracín Orio, Andrea Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaAmerican Society for Microbiology2008-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241124Cortes, Paulo; Albarracín Orio, Andrea Georgina; Regueira, Mabel; Piñas, German Eduardo; Echenique, Jose Ricardo; Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina; American Society for Microbiology; Journal of Clinical Microbiology; 46; 6; 6-2008; 1930-19340095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.02318-07info:eu-repo/semantics/altIdentifier/doi/10.1128/jcm.02318-07info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:26Zoai:ri.conicet.gov.ar:11336/241124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:27.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
title |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
spellingShingle |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina Cortes, Paulo STREPTOCOCCUS PNEUMONIAE H+-ATPASE OPTOQUIN RESISTANCE atpAC GENES |
title_short |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
title_full |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
title_fullStr |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
title_full_unstemmed |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
title_sort |
Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina |
dc.creator.none.fl_str_mv |
Cortes, Paulo Albarracín Orio, Andrea Georgina Regueira, Mabel Piñas, German Eduardo Echenique, Jose Ricardo |
author |
Cortes, Paulo |
author_facet |
Cortes, Paulo Albarracín Orio, Andrea Georgina Regueira, Mabel Piñas, German Eduardo Echenique, Jose Ricardo |
author_role |
author |
author2 |
Albarracín Orio, Andrea Georgina Regueira, Mabel Piñas, German Eduardo Echenique, Jose Ricardo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
STREPTOCOCCUS PNEUMONIAE H+-ATPASE OPTOQUIN RESISTANCE atpAC GENES |
topic |
STREPTOCOCCUS PNEUMONIAE H+-ATPASE OPTOQUIN RESISTANCE atpAC GENES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains. Fil: Cortes, Paulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Albarracín Orio, Andrea Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina |
description |
Optochin susceptibility is a key test used for pneumococcal diagnosis, but optochin-resistant (Optr) pneumococci have been reported in the last 2 decades. In this work, we characterized eight Optr clinical strains which presented a new mutation, G47V, a predominant A49S mutation (recently reported in Brazil) and A49T. These mutations were found in the c subunit of the F0F1 ATPase encoded by the atpC gene, and W206C was found in the a subunit encoded by the atpA gene. The Optr clinical isolates were analyzed by BOX PCR, multilocus sequence typing, and serotype and antimicrobial resistance profiles, and they showed no epidemiological relationship. To characterize the Optr mutations that could emerge among clinical strains, we studied a pool of spontaneous Optr colonies obtained in vitro from the virulent D39 strain. We compared the atpAC mutations of these Optr pneumococci (with or without passage through C57BL/6 mice) with those described in the clinical isolates. This analysis revealed three new mutations, G47V and L26M in the c subunit and L184S in the a subunit. Most of the mutations identified in the laboratory-generated Optr strains were also found in clinical strains, with the exception of the L26M and L184S mutations, and we suppose that both mutations could emerge among invasive strains in the future. Considering that atpAC are essential genes, we propose that all spontaneous mutations that confer in vitro optochin resistance would not present severe physiological alterations in S. pneumoniae and may be carried by circulating pneumococcal strains. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/241124 Cortes, Paulo; Albarracín Orio, Andrea Georgina; Regueira, Mabel; Piñas, German Eduardo; Echenique, Jose Ricardo; Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina; American Society for Microbiology; Journal of Clinical Microbiology; 46; 6; 6-2008; 1930-1934 0095-1137 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/241124 |
identifier_str_mv |
Cortes, Paulo; Albarracín Orio, Andrea Georgina; Regueira, Mabel; Piñas, German Eduardo; Echenique, Jose Ricardo; Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina; American Society for Microbiology; Journal of Clinical Microbiology; 46; 6; 6-2008; 1930-1934 0095-1137 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.02318-07 info:eu-repo/semantics/altIdentifier/doi/10.1128/jcm.02318-07 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613763712417792 |
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13.070432 |