Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis

Autores
Hernandez-Neuta, Iván; Varela, Andres; Martin, Anandi; von Groll, Andrea; Jureen, Pontus; López, Beatriz; Imperiale, Belen; Skenders, Girts; Ritacco, Viviana; Hoffner, Sven; Morcillo, Nora; Palomino, Juan Carlos; Del Portillo, Patricia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.
Fil: Varela, Andres. Corporacion CorpoGen; Colombia.
Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina.
Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia.
Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina.
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia.
on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources.
Fuente
Journal of Clinical Microbiology 2010; 48(12):4386-4391.
Materia
Rifampin
Sondas de Oligonucleótidos
Mycobacterium tuberculosis
Tuberculosis Resistente a Múltiples Medicamentos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/307

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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/307
network_acronym_str SGCANLIS
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosisHernandez-Neuta, IvánVarela, AndresMartin, Anandivon Groll, AndreaJureen, PontusLópez, BeatrizImperiale, BelenSkenders, GirtsRitacco, VivianaHoffner, SvenMorcillo, NoraPalomino, Juan CarlosDel Portillo, PatriciaRifampinSondas de OligonucleótidosMycobacterium tuberculosisTuberculosis Resistente a Múltiples MedicamentosFil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.Fil: Varela, Andres. Corporacion CorpoGen; Colombia.Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina.Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia.Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia.Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina.Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia.on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources.American Society for Microbiology2010-12info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-660Xhttp://sgc.anlis.gob.ar/handle/123456789/307http://jcm.asm.org/content/48/12/4386.full.pdf+htmlJournal of Clinical Microbiology 2010; 48(12):4386-4391.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of clinical microbiologyenginfo:eu-repo/semantics/openAccess2025-09-04T11:15:39Zoai:sgc.anlis.gob.ar:123456789/307Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:15:39.735Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
title Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
spellingShingle Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
Hernandez-Neuta, Iván
Rifampin
Sondas de Oligonucleótidos
Mycobacterium tuberculosis
Tuberculosis Resistente a Múltiples Medicamentos
title_short Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
title_full Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
title_fullStr Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
title_full_unstemmed Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
title_sort Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Hernandez-Neuta, Iván
Varela, Andres
Martin, Anandi
von Groll, Andrea
Jureen, Pontus
López, Beatriz
Imperiale, Belen
Skenders, Girts
Ritacco, Viviana
Hoffner, Sven
Morcillo, Nora
Palomino, Juan Carlos
Del Portillo, Patricia
author Hernandez-Neuta, Iván
author_facet Hernandez-Neuta, Iván
Varela, Andres
Martin, Anandi
von Groll, Andrea
Jureen, Pontus
López, Beatriz
Imperiale, Belen
Skenders, Girts
Ritacco, Viviana
Hoffner, Sven
Morcillo, Nora
Palomino, Juan Carlos
Del Portillo, Patricia
author_role author
author2 Varela, Andres
Martin, Anandi
von Groll, Andrea
Jureen, Pontus
López, Beatriz
Imperiale, Belen
Skenders, Girts
Ritacco, Viviana
Hoffner, Sven
Morcillo, Nora
Palomino, Juan Carlos
Del Portillo, Patricia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rifampin
Sondas de Oligonucleótidos
Mycobacterium tuberculosis
Tuberculosis Resistente a Múltiples Medicamentos
topic Rifampin
Sondas de Oligonucleótidos
Mycobacterium tuberculosis
Tuberculosis Resistente a Múltiples Medicamentos
dc.description.none.fl_txt_mv Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.
Fil: Varela, Andres. Corporacion CorpoGen; Colombia.
Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina.
Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia.
Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina.
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia.
on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources.
description Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-660X
http://sgc.anlis.gob.ar/handle/123456789/307
http://jcm.asm.org/content/48/12/4386.full.pdf+html
identifier_str_mv 1098-660X
url http://sgc.anlis.gob.ar/handle/123456789/307
http://jcm.asm.org/content/48/12/4386.full.pdf+html
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of clinical microbiology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Journal of Clinical Microbiology 2010; 48(12):4386-4391.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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