Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis
- Autores
- Hernandez-Neuta, Iván; Varela, Andres; Martin, Anandi; von Groll, Andrea; Jureen, Pontus; López, Beatriz; Imperiale, Belen; Skenders, Girts; Ritacco, Viviana; Hoffner, Sven; Morcillo, Nora; Palomino, Juan Carlos; Del Portillo, Patricia
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.
Fil: Varela, Andres. Corporacion CorpoGen; Colombia.
Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina.
Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia.
Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina.
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.
Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia.
on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources. - Fuente
- Journal of Clinical Microbiology 2010; 48(12):4386-4391.
- Materia
-
Rifampin
Sondas de Oligonucleótidos
Mycobacterium tuberculosis
Tuberculosis Resistente a Múltiples Medicamentos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/307
Ver los metadatos del registro completo
id |
SGCANLIS_34b9f3cfde8b221a9538003b9426a4da |
---|---|
oai_identifier_str |
oai:sgc.anlis.gob.ar:123456789/307 |
network_acronym_str |
SGCANLIS |
repository_id_str |
a |
network_name_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
spelling |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosisHernandez-Neuta, IvánVarela, AndresMartin, Anandivon Groll, AndreaJureen, PontusLópez, BeatrizImperiale, BelenSkenders, GirtsRitacco, VivianaHoffner, SvenMorcillo, NoraPalomino, Juan CarlosDel Portillo, PatriciaRifampinSondas de OligonucleótidosMycobacterium tuberculosisTuberculosis Resistente a Múltiples MedicamentosFil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia.Fil: Varela, Andres. Corporacion CorpoGen; Colombia.Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina.Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia.Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia.Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina.Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica.Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia.on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources.American Society for Microbiology2010-12info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-660Xhttp://sgc.anlis.gob.ar/handle/123456789/307http://jcm.asm.org/content/48/12/4386.full.pdf+htmlJournal of Clinical Microbiology 2010; 48(12):4386-4391.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of clinical microbiologyenginfo:eu-repo/semantics/openAccess2025-09-04T11:15:39Zoai:sgc.anlis.gob.ar:123456789/307Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:15:39.735Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
title |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
spellingShingle |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis Hernandez-Neuta, Iván Rifampin Sondas de Oligonucleótidos Mycobacterium tuberculosis Tuberculosis Resistente a Múltiples Medicamentos |
title_short |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
title_full |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
title_fullStr |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
title_full_unstemmed |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
title_sort |
Rifampin-Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis |
dc.creator.none.fl_str_mv |
Hernandez-Neuta, Iván Varela, Andres Martin, Anandi von Groll, Andrea Jureen, Pontus López, Beatriz Imperiale, Belen Skenders, Girts Ritacco, Viviana Hoffner, Sven Morcillo, Nora Palomino, Juan Carlos Del Portillo, Patricia |
author |
Hernandez-Neuta, Iván |
author_facet |
Hernandez-Neuta, Iván Varela, Andres Martin, Anandi von Groll, Andrea Jureen, Pontus López, Beatriz Imperiale, Belen Skenders, Girts Ritacco, Viviana Hoffner, Sven Morcillo, Nora Palomino, Juan Carlos Del Portillo, Patricia |
author_role |
author |
author2 |
Varela, Andres Martin, Anandi von Groll, Andrea Jureen, Pontus López, Beatriz Imperiale, Belen Skenders, Girts Ritacco, Viviana Hoffner, Sven Morcillo, Nora Palomino, Juan Carlos Del Portillo, Patricia |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Rifampin Sondas de Oligonucleótidos Mycobacterium tuberculosis Tuberculosis Resistente a Múltiples Medicamentos |
topic |
Rifampin Sondas de Oligonucleótidos Mycobacterium tuberculosis Tuberculosis Resistente a Múltiples Medicamentos |
dc.description.none.fl_txt_mv |
Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia. Fil: Varela, Andres. Corporacion CorpoGen; Colombia. Fil: Martin, Anandi. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica. Fil: von Groll, Andrea. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica. Fil: Jureen, Pontus. Swedish Institute for Infectious Disease Control; Suecia. Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Imperiale, Belen. Hospital Dr. Cetrángolo; Argentina. Fil: Skenders, Girts. State Agency of Tuberculosis and Lung Diseases; Letonia. Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia. Fil: Morcillo, Nora. Hospital Dr. Cetrángolo; Argentina. Fil: Palomino, Juan Carlos. Institute of Tropical Medicine. Mycobacteriology Unit; Bélgica. Fil: Del Portillo, Patricia. Corporacion CorpoGen; Colombia. on of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrug-resistant tuberculosis but that cannot afford expensive commercial tests because of limited resources. |
description |
Fil: Hernandez-Neuta, Iván. Corporacion CorpoGen; Colombia. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1098-660X http://sgc.anlis.gob.ar/handle/123456789/307 http://jcm.asm.org/content/48/12/4386.full.pdf+html |
identifier_str_mv |
1098-660X |
url |
http://sgc.anlis.gob.ar/handle/123456789/307 http://jcm.asm.org/content/48/12/4386.full.pdf+html |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of clinical microbiology |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
Journal of Clinical Microbiology 2010; 48(12):4386-4391. reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
reponame_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
collection |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
institution |
ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
_version_ |
1842344418556772352 |
score |
12.623145 |